These Product FAQs pertain to RUO products. These products are for Research Use Only. Not for use in diagnostic procedures. If using an IVD product, please refer to your specific product information.
What is the doubling time of Drosophila S2 cells?
When grown at the optimal temperature of 27-28ºC, Drosophila S2 cells grow very quickly, with an approximate 14-16 hr doubling time. The cells can also be grown at 22-25ºC with doubling time of 24hrs. Humidity is an issue, so a shallow pan of water can be left in the incubator, but the incubator does not need to be turned on.
Growth medium is also a factor in doubling time - the doubling times listed here are for Sf900 II medium.
The cells can reach maximum densities of 1 to 2.5 x 10E7 cells/ml in shake flask cultures.
Answer Id: E3312
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