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Specific ROS Detection%2C DAF-FM

Citations & References

Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.

  • Authors: Kim YC, Kim BG, Lee JH,
  • Journal: PLoS One (2012) 7:e35399-e35399
  • PubMed ID: 22623951

Product FAQ

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

Answer

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

Answer Id: E6451

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Manual / Product Insert

CellROX Oxidative Stress Reagents

Version: 6 May 2012

Citations & References

Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation.

  • Authors: Franco R, Panayiotidis MI, Cidlowski JA,
  • Journal: J Biol Chem (2007) 282:30452-30465
  • PubMed ID: 17724027

Citations & References

Role of ROS and MAPK in TPA-induced ICAM-1 expression in the myeloid ML-1 cell line.

  • Authors: Traore K, Sharma RB, Burek CL, Trush MA
  • Journal: J Cell Biochem 2007; (100):4 1010-1021
  • PubMed ID: 17131377

Citations & References

Amyloid beta-peptide impairs ion-motive ATPase activities: evidence for a role in loss of neuronal Ca2+ homeostasis and cell death.

  • Authors: Mark RJ, Hensley K, Butterfield DA, Mattson MP
  • Journal: J Neurosci (1995) 15:6239-6249
  • PubMed ID: 7666206

Citations & References

High lateral mobility of endogenous and transfected alkaline phosphatase: a phosphatidylinositol-anchored membrane protein.

  • Authors: Noda M, Yoon K, Rodan GA, Koppel DE
  • Journal: J Cell Biol (1987) 105:1671-1677
  • PubMed ID: 2889741
Catalog #

Citations & References

Activation of endothelial TRPV4 channels mediates flow-induced dilation in human coronary arterioles: role of Ca2+ entry and mitochondrial ROS signaling.

  • Authors: Bubolz AH, Mendoza SA, Zheng X, Zinkevich NS, Li R, Gutterman DD, Zhang DX,
  • Journal: Am J Physiol Heart Circ Physiol (2012) 302:H634-H642
  • PubMed ID: 22140047

Citations & References

Distinct mechanisms for aerenchyma formation in leaf sheaths of rice genotypes displaying a quiescence or escape strategy for flooding tolerance.

  • Authors: Parlanti S, Kudahettige NP, Lombardi L, Mensuali-Sodi A, Alpi A, Perata P, Pucciariello C,
  • Journal: Ann Bot (2011) 107:1335-1343
  • PubMed ID: 21489969

Citations & References

Resistance to cerebral ischemic injury in UCP2 knockout mice: evidence for a role of UCP2 as a regulator of mitochondrial glutathione levels.

  • Authors: de Bilbao F, Arsenijevic D, Vallet P, Hjelle OP, Ottersen OP, Bouras C, Raffin Y, Abou K, Langhans W, Collins S, Plamondon J, Alves-Guerra MC, Haguenauer A, Garcia I, Richard D, Ricquier D, Giannakopoulos P
  • Journal: J Neurochem (2004) 89:1283-1292
  • PubMed ID: 15147521
Catalog #

Citations & References

Persistent activation of ERK contributes to glutamate-induced oxidative toxicity in a neuronal cell line and primary cortical neuron cultures.

  • Authors: Stanciu M, Wang Y, Kentor R, Burke N, Watkins S, Kress G, Reynolds I, Klann E, Angiolieri MR, Johnson JW, DeFranco DB
  • Journal: J Biol Chem (2000) 275:12200-12206
  • PubMed ID: 10766856

Citations & References

SOD2 contributes to anti-oxidative capacity in rabbit corneal endothelial cells.

  • Authors: Liu C, Ogando D, Bonanno JA,
  • Journal: Mol Vis (2011) 17:2473-2481
  • PubMed ID: 21976958