Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.

Citations & References

  • Authors: Kim YC, Kim BG, Lee JH,
  • Journal: PLoS One (2012) 7:e35399-e35399
  • PubMed ID: 22623951

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

Product FAQ

Answer

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

Answer Id: E6451

Was this answer helpful?

Yes
No
Thank you for your response

Glutathione depletion is necessary for apoptosis in lymphoid cells independent of reactive oxygen species formation.

Citations & References

  • Authors: Franco R, Panayiotidis MI, Cidlowski JA,
  • Journal: J Biol Chem (2007) 282:30452-30465
  • PubMed ID: 17724027

CellROX Oxidative Stress Reagents

Manual / Product Insert

Version: 6 May 2012

Troglitazone-induced hepatic necrosis in an animal model of silent genetic mitochondrial abnormalities.

Citations & References

  • Authors: Ong MM, Latchoumycandane C, Boelsterli UA,
  • Journal: Toxicol Sci (2007) 97:205-213
  • PubMed ID: 17150972

Mitochondria-targeted superoxide dismutase (SOD2) regulates radiation resistance and radiation stress response in HeLa cells.

Citations & References

  • Authors: Hosoki A, Yonekura S, Zhao QL, Wei ZL, Takasaki I, Tabuchi Y, Wang LL, Hasuike S, Nomura T, Tachibana A, Hashiguchi K, Yonei S, Kondo T, Zhang-Akiyama QM,
  • Journal: J Radiat Res (2012) 53:58-71
  • PubMed ID: 22302046

Resolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.

Citations & References

  • Authors: Pung YF, Rocic P, Murphy MP, Smith RA, Hafemeister J, Ohanyan V, Guarini G, Yin L, Chilian WM,
  • Journal: Arterioscler Thromb Vasc Biol (2012) 32:325-334
  • PubMed ID: 22155454

Information Card: Human Protein Kinase Array

Manual / Product Insert

Version: AB
Catalog #

Ca2+ regulates reactive oxygen species production and pH during mechanosensing in Arabidopsis roots.

Citations & References

  • Authors: Monshausen GB, Bibikova TN, Weisenseel MH, Gilroy S,
  • Journal: Plant Cell (2009) 21:2341-2356
  • PubMed ID: 19654264
Catalog #

Methylmercury-induced reactive oxygen species formation in neonatal cerebral astrocytic cultures is attenuated by antioxidants.

Citations & References

  • Authors: Shanker G, Aschner M
  • Journal: Brain Res Mol Brain Res (2003) 110:85-91
  • PubMed ID: 12573536
Catalog #

Activation of endothelial TRPV4 channels mediates flow-induced dilation in human coronary arterioles: role of Ca2+ entry and mitochondrial ROS signaling.

Citations & References

  • Authors: Bubolz AH, Mendoza SA, Zheng X, Zinkevich NS, Li R, Gutterman DD, Zhang DX,
  • Journal: Am J Physiol Heart Circ Physiol (2012) 302:H634-H642
  • PubMed ID: 22140047

Resistance to cerebral ischemic injury in UCP2 knockout mice: evidence for a role of UCP2 as a regulator of mitochondrial glutathione levels.

Citations & References

  • Authors: de Bilbao F, Arsenijevic D, Vallet P, Hjelle OP, Ottersen OP, Bouras C, Raffin Y, Abou K, Langhans W, Collins S, Plamondon J, Alves-Guerra MC, Haguenauer A, Garcia I, Richard D, Ricquier D, Giannakopoulos P
  • Journal: J Neurochem (2004) 89:1283-1292
  • PubMed ID: 15147521
Catalog #

The role of reactive oxygen species in arsenite and monomethylarsonous acid-induced signal transduction in human bladder cells: acute studies.

Citations & References

  • Authors: Eblin KE, Hau AM, Jensen TJ, Futscher BW, Gandolfi AJ
  • Journal: Toxicology 2008 1:47-54
  • PubMed ID: 18588940

Morphological and flow-cytometric analysis of haemin-induced human neutrophil activation: implications for transfusion-related acute lung injury.

Citations & References

  • Authors: Kono M, Saigo K, Takagi Y, Kawauchi S, Wada A, Hashimoto M, Sugimoto T, Takenokuchi M, Morikawa T, Funakoshi K,
  • Journal: Blood Transfus (2013) 11:53-60
  • PubMed ID: 22790260
Catalog #