Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.

Citations & References

  • Authors: Kim YC, Kim BG, Lee JH,
  • Journal: PLoS One (2012) 7:e35399-e35399
  • PubMed ID: 22623951

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

Product FAQ

Answer

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

Answer Id: E6451

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Additional Document: Reference Bibliography for Amplitaq and AmpliTaq Gold 2005-05-06 v1.1

Product Literature

On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy.

Citations & References

  • Authors: Price M, Kessel D,
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  • PubMed ID: 21054079

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Citations & References

  • Authors: Ichiba H, Hanami K, Yagasaki K, Tanaka M, Ito H, Fukushima T,
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  • PubMed ID: 22460428
Catalog #

Additional Document: Others (most referenced journals) 2005-05

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Morphological and flow-cytometric analysis of haemin-induced human neutrophil activation: implications for transfusion-related acute lung injury.

Citations & References

  • Authors: Kono M, Saigo K, Takagi Y, Kawauchi S, Wada A, Hashimoto M, Sugimoto T, Takenokuchi M, Morikawa T, Funakoshi K,
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  • PubMed ID: 22790260
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Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species.

Citations & References

  • Authors: Setsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T
  • Journal: J Biol Chem (2003) 278:3170-3175
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Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues

Citations & References

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Transcriptional profiling of maturing tomato (Solanum lycopersicum L.) microspores reveals the involvement of heat shock proteins, ROS scavengers, hormones, and sugars in the heat stress response.

Citations & References

  • Authors: Frank G, Pressman E, Ophir R, Althan L, Shaked R, Freedman M, Shen S, Firon N
  • Journal: J Exp Bot 2009; (60):13 3891-3908
  • PubMed ID: 19628571

Cocaine-mediated enhancement of Tat toxicity in rat hippocampal cell cultures: the role of oxidative stress and D1 dopamine receptor.

Citations & References

  • Authors: Aksenov MY, Aksenova MV, Nath A, Ray PD, Mactutus CF, Booze RM
  • Journal: Neurotoxicology (2006) 27:217-228
  • PubMed ID: 16386305

Gene and protein expression in the epididymis of infertile c-ros receptor tyrosine kinase-deficient mice.

Citations & References

  • Authors: Cooper TG, Wagenfeld A, Cornwall GA, Hsia N, Chu ST, Orgebin-Crist MC, Drevet J, Vernet P, Avram C, Nieschlag E, Yeung CH
  • Journal: Biol Reprod 2003; (69):5 1750-1762
  • PubMed ID: 12890734

COX-2 induction by unconjugated bile acids involves reactive oxygen species-mediated signalling pathways in Barrett's oesophagus and oesophageal adenocarcinoma.

Citations & References

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