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specific ROS detection%2C APF

Citations & References

Thymosin ß10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production.

  • Authors: Kim YC, Kim BG, Lee JH,
  • Journal: PLoS One (2012) 7:e35399-e35399
  • PubMed ID: 22623951

Product FAQ

I am using the reactive oxygen species detection dye, Dihydrorhodamine 123, and when I put the dye in a solution of water and illuminate with UV light, I see a quick change in signal, even without cells. What is happening and how can I stop this?

Answer

You cannot stop it. Any time you illuminate a dye, including this one, photodegradation of the dye will lead to the production of singlet oxygen and free radicals. This in turn causes many ROS indicators, like this one, to become oxidized. If you are testing ROS using UV illumination, all you can do is use this as a baseline and subtract the signal from the sample with cells. The best you can do is reduce the illumination intensity (though this will also reduce the specific signal emission).

Answer Id: E6451

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Product Literature

Additional Document: Basic and Human Disease research - Journals A-I 2005-05

Product Literature

Additional Document: Reference Bibliography for Amplitaq and AmpliTaq Gold 2005-05-06 v1.1

Product Literature

Additional Document: Others (most referenced journals) 2005-05

Citations & References

On the use of fluorescence probes for detecting reactive oxygen and nitrogen species associated with photodynamic therapy.

  • Authors: Price M, Kessel D,
  • Journal: J Biomed Opt (2010) 15:051605-051605
  • PubMed ID: 21054079

Citations & References

Morphological and flow-cytometric analysis of haemin-induced human neutrophil activation: implications for transfusion-related acute lung injury.

  • Authors: Kono M, Saigo K, Takagi Y, Kawauchi S, Wada A, Hashimoto M, Sugimoto T, Takenokuchi M, Morikawa T, Funakoshi K,
  • Journal: Blood Transfus (2013) 11:53-60
  • PubMed ID: 22790260
Catalog #

Citations & References

A novel flow-injection analysis system for evaluation of antioxidants by using sodium dichloroisocyanurate as a source of hypochlorite anion.

  • Authors: Ichiba H, Hanami K, Yagasaki K, Tanaka M, Ito H, Fukushima T,
  • Journal: Drug Discov Ther (2012) 6:44-48
  • PubMed ID: 22460428
Catalog #

Citations & References

Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species.

  • Authors: Setsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T
  • Journal: J Biol Chem (2003) 278:3170-3175
  • PubMed ID: 12419811

Citations & References

Role of Phospholipase D in Parathyroid Hormone Type 1 Receptor Signaling and Trafficking

  • Authors: Garrido, JL; Wheeler, D; Vega, LL; Friedman, PA; Romero, G
  • Journal: MOLECULAR ENDOCRINOLOGY 2009 12:2048-2059

Citations & References

Survival factor-insensitive generation of reactive oxygen species induced by serum deprivation in neuronal cells.

  • Authors: Satoh T, Sakai N, Enokido Y, Uchiyama Y, Hatanaka H
  • Journal: Brain Res (1996) 733:9-14
  • PubMed ID: 8891242

Citations & References

Characterization of acetylcholine receptor isolated from Torpedo californica electroplax through the use of an easily removable detergent, beta-D-octylglucopyranoside.

  • Authors: Gonzalez-Ros JM, Paraschos A, Farach MC, Martinez-Carrion M
  • Journal: Biochim Biophys Acta (1981) 643:407-420
  • PubMed ID: 7225389

Citations & References

Transcriptomic footprints disclose specificity of reactive oxygen species signaling in Arabidopsis.

  • Authors: Gadjev I, Vanderauwera S, Gechev TS, Laloi C, Minkov IN, Shulaev V, Apel K, Inzé D, Mittler R, Van Breusegem F
  • Journal: Plant Physiol 2006; (141):2 436-445
  • PubMed ID: 16603662

Citations & References

Interleukin-6 counteracts therapy-induced cellular oxidative stress in multiple myeloma by up-regulating manganese superoxide dismutase.

  • Authors: Brown CO, Salem K, Wagner BA, Bera S, Singh N, Tiwari A, Choudhury A, Buettner GR, Goel A,
  • Journal: Biochem J (2012) 444:515-527
  • PubMed ID: 22471522