Lot # 1097932

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Lot # 1098051

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CT1428-TS

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Lot # 493530-COA

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Lot # 1098052

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I am getting poor resolution or see smearing of bands when using my E-Gel™ EX Agarose Gels. Why is this?

Product FAQ

Answer

Here are some possibilities and suggestions to resolve the problem:

  • The sample is overloaded. Do not load more than the recommended amount.
  • A high salt concentration. Dilute the samples.
  • The E-Gel™ EX Agarose Gels were prerun; this is not recommended for these gels.
  • A very low volume of sample was loaded or the sample was not loaded properly.
  • Bubbles may have been introduced while loading the samples. Bubbles will cause band distortion. Load the recommended sample volume based on the gel type and loading method. For proper band separation, we recommend keeping sample volumes uniform. Load deionized water or TE into any empty wells, and avoid introducing bubbles.
  • The gel was not electrophoresed immediately after sample loading. Run your sample within 1 minute of loading it.
  • The E-Gel™ Agarose Gel may have been used beyond its expiration date. Check the expiration date.
  • A longer electrophoresis run time or high current was used during the run. Longer run times cause an increase in the current, resulting in poor band migration or a melted gel. Do not run the gel longer than the recommended time for each gel type.

    Answer Id: E8023

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  • CT1429-TS

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    Lot # 622620-COA

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    Lot # 1382553

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    Lot # 1098053

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    Lot # 1106043

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    I am trying to run my E-Gel™ software on my Mac™ computer, but it’s not working. What should I do?

    Product FAQ

    Answer

    We do not currently offer software that is compatible with Mac™ computers. Please install the software on a 32-bit or 64-bit PC running either a Windows™ XP Professional or Windows™ 7 Professional operating system.

    Answer Id: E8024

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    Lot # 1336347-COA

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    CT1430-TS

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    Lot # 633526-COA

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