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DN101504

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I see that the pSectag2 vectors carry the Zeocin™ antibiotic-resistance gene driven by the EM7 promoter. Can I use Zeocin™ antibiotic for selection when I propagate these vectors in E. coli?

Product FAQ

Answer

Yes, you can use Zeocin™ antibiotic for selection in E. coli. However, keep in mind that for Zeocin™ antibiotic to be active, the salt concentration of the medium must remain low (<90 mM) and the pH must be 7.5. Prepare LB broth and LB agar plates using low-salt (5 g NaCl/liter) LB.

Answer Id: E9212

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DN101515

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Can I propagate my pSecTag2 vector in E. coli containing the Tn5 gene?

Product FAQ

Answer

pSectag2 vectors have the Zeocin™ antibiotic-resistance marker for selection in E. coli, and any E. coli strain that contains the complete Tn5 transposable element (i.e., DH5alphaF’IQ, SURE, SURE2) encodes the ble (bleomycin) resistance gene that confers resistance to the Zeocin™ antibiotic. Hence, for the most efficient selection, we highly recommend choosing an E. coli strain that does not contain the Tn5 gene.

Answer Id: E9213

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