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What can the ARES™ Alexa Fluor™ DNA Labeling Kits be used for?

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The ARES™ Alexa Fluor™ DNA Labeling Kits provides a versatile, two-step method for labeling DNA with our Alexa Fluor™ dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our amine-reactive Alexa Fluor™ dyes. The labeled probes can be used for:

  • Fluorescence in situ hybridization (FISH). See Buster DW, Daniel SG, Nguyen HQ, et al. (2013) SCFSlimb ubiquitin ligase suppresses condensin II–mediated nuclear reorganization by degrading Cap-H2, J Cell Biol 201(1):49-63.
  • Microarray techniques. See Shin HH, Hwang BH, Seo JH et al. (2014) Specific discrimination of three pathogenic Salmonella enterica subsp. enterica serotypes by carB-based oligonucleotide microarray. Appl Environ Microbiol 80(1):366-373.

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    What is the optimal degree of labeling (dye molecules per 100 base pairs) for labeled probes produced by the ARES™ Alexa Fluor™ DNA Labeling Kits?

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    A dye-to-base ratio of 1:12 to 1:35 is a suitable range for Alexa Fluor™ dye labeling of cDNA for microarray and FISH (fluorescence in situ hybridization) applications.

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