I used the PowerEase™ 300W Power Supply for Native-PAGE electrophoresis and my power supply turned off in the middle of the gel run. Did I do something wrong?

Product FAQ

Answer

During NativePAGE™ electrophoresis, it is common for the current to drop below 1 mA. The PowerEase™ 300W Power Supply will register this as a “No Load” error and automatically shut off, resulting in the stopping of the gel run. Since there is no way to disable/turn off the “Load Check” feature in the PowerEase™ 300W Power Supply, we do not recommend using it for Native-PAGE electrophoresis. Alternatively, the ZOOM™ Dual Power Supply is capable of disabling the “Load Check” feature and is our recommended power supply for NativePAGE™ applications.

Answer Id: E11016

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I am using the PowerEase™ 90W Power Supply and am seeing that the LCD screen remains blank and the fan does not run when the power is turned on. What should I do?

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Answer

Here are possible causes and solutions:

- AC power cord is not connected. Check AC power cord connections at both ends. Use the correct cord.
- The fuse has blown. Replace the fuse.

Answer Id: E11017

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My PowerEase™ 90W Power Supply stopped functioning with an alarm and the screen displays “No Load”. What is possibly wrong?

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Answer

Here are the possible causes and suggested solutions:

- Electrophoresis leads are not connected to the power supply or to the electrophoresis unit(s), or there is a broken circuit in the electrophoresis cell. Check the connections to the power supply and on your electrophoresis cell to make sure the connection is intact; check condition of wires in electrophoresis unit. Close the circuit by reconnecting the cables. Press Start/Pause to restart the run.
- High resistance due to tape left on a pre-cast gel, incorrect buffer concentration, or incorrect buffer volumes in the electrophoresis cell. Correct the condition by making sure the tape is removed from the pre-cast gel, buffers are prepared correctly, and the recommended volume of buffer is added to the electrophoresis unit.
- Current has dropped below acceptable rating (4 mA). The power supply has a built-in “No Load Detection” feature that can detect when the current drops below the acceptable rating of 4 mA.

Answer Id: E11018

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How many SNP and CN probes are included on CytoScan™ 750K Array?

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Answer

CytoScan™ 750K Array includes a total of 1.15 million probes: 1 probe per allele in triplicate for 200,000 SNP probes and 550,000 non-polymorphic probes.

Answer Id: E13837

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I applied an inhibitor/drug/etc. to the GeneArt™ Engineered cell line and did not observe the phenotype I expected. Why is this?

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Answer

With all of our cell lines, we only carry out genomic validation via sequencing/PCR to determine that the mutation is present. We do not perform functional validation. You can try a different genotype/clone if available.

Answer Id: E10495

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Can the attB primers anneal in a non-specific manner?

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Answer

No, attB primers are highly specific under standard PCR conditions. We have amplified from RNA (RT-PCR), cDNA libraries, genomic DNA, and plasmid templates without any specificity problems.

Answer Id: E3199

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I am using your PowerEase™ 90W Power Supply. The operation stopped with an alarm and the screen displays: “Over Voltage” What is the problem?

Product FAQ

Answer

Here are some suggestions: - Verify that the running buffer is correct.
- Verify that all cables are attached correctly.
- Turn the Power switch off and on again; restart application.
- If you cannot restart the instrument, turn off the power, disconnect the power cord from the outlet, and contact Technical Service by sending an e-mail to techsupport@thermofisher.com.

Answer Id: E11019

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Do you offer a recalibration service for the PowerEase™ 500 Power Supply?

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Answer

The PowerEase™ 500 Power Supply has been discontinued as of December 31, 2013. We do not offer a recalibration service for this instrument.

Answer Id: E11035

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What array software is available for genoytping, copy number analysis, and analysis for CytoScan™ Optima Suite?

Product FAQ

Answer

All genotyping, copy number analysis, and analysis for CytoScan™ Optima Suite is done with ChAS 3.1. This product is not intended for genome-wide association studies

Answer Id: E13838

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Do your precast protein gels contain SDS?

Product FAQ

Answer

Our precast protein gels do not contain SDS but they can be run under denaturing conditions when used with the appropriate denaturing running buffer.
Note: NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels, and Thermo Scientific™ Precise™ Tris-HEPES gels cannot be run under native conditions; they can only be run under denaturing conditions.

*Invitrogen™ Tris-Glycine gels: For Native electrophoresis, use Invitrogen™ Tris-Glycine Native Running Buffer. For Denaturing electrophoresis, use Invitrogen™ Tris-Glycine SDS Running Buffer

*NuPAGE™ Tris-Acetate gels: For Native electrophoresis, use Invitrogen™ Tris-Glycine Native Running Buffer. For Denaturing electrophoresis, use NuPAGE™ Tris-Acetate SDS Running Buffer

*NuPAGE™ Bis-Tris gels: For Denaturing electrophoresis, use NuPAGE™ MOPS-SDS Running Buffer or NuPAGE™ MES-SDS Running Buffer

*Bolt™ Bis-Tris Plus gels: For Denaturing electrophoresis, use Bolt™ MOPS SDS Running Buffer or Bolt™ MES SDS Running Buffer

*Thermo Scientific™ Precise™ Tris-Glycine gels: For Native electrophoresis, use Tris-Glycine SDS Running Buffer without SDS added. For Denaturing electrophoresis, use Tris-Glycine SDS Running Buffer.

*Thermo Scientific™ Precise™ Tris-HEPES gels: For Denaturing electrophoresis, use Tris-HEPES SDS Running Buffer.

Answer Id: E10496

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Can I use TB or rich media with the BenchPro™2100 instrument?

Product FAQ

Answer

We do not recommend the use of TB or rich media. A high cell density will clog the membrane and decrease DNA yield from the purification. For best results, set up the purification as indicated in the manual.

Answer Id: E7779

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Will Gateway™ att sites affect the expression of my protein?

Product FAQ

Answer

Expression experiments have shown that the extra amino acids contributed by the attB site to a fusion protein will most likely have no effect on protein expression levels or stability. In addition, they do not appear to have any effect on two-hybrid interactions in yeast. However, as is true with the addition of any extra sequences that result from tags, the possible effects will be protein-dependent.

Answer Id: E3200

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My PowerEase™ 90W Power Supply stopped working with an alarm and the screen displays “Leakage”. Can you please help?

Product FAQ

Answer

This is most likely due to a ground leak detected during the run. Check the electrophoresis system for improper grounding and restart the power supply by turning the Power switch off and on.

Answer Id: E11020

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I am using your PowerEase™ 500 Power Supply and just noticed that the high voltage indicator light is on. What is the problem?

Product FAQ

Answer

The high voltage indicator light (amber or red) is a warning light that indicates that the power source is in operation. Do not unplug the leads when this light is on.

Answer Id: E11036

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What is the CytoScan™ Optima Array content?

Product FAQ

Answer

CytoScan™ Optima Array content has been selected empirically from CytoScan™ HD Array and was designed for prenatal and miscarriage sample analysis.

The functional resolution of CytoScan™ Optima Suite is
-1 MB for losses and 2 MB for gains genome wide
-5 MB for LOH/AOH
-100 kb resolution in 396 relevant regions for prenatal research

Answer Id: E13839

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