Product FAQ

For the CytoScan™ assay, can a different magnetic rack be used instead of the recommended MagnaRack™ magnetic stand?

Answer

Yes, the assay is currently validated for the following magnetic racks:
DynaMag™-2 Magnet (Cat. No. 123-21D)
MagnaRack™ (Cat. No. CS15000)

Answer Id: E13971

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Product FAQ

What is the typical yield range from PCR product purification?

Answer

The minimum recommended yield is 2.5 μg/μL for a sample. Yields can range from 3.0-4.5 μg/μL, and the average yield for seven or more samples processed in a run (not including the negative control) should be greater than 3.0 μg/μL. If the average yield is below this, consult the troubleshooting section of the CytoScan™ Assay User Manual (Cat. No. 703038, https://tools.thermofisher.com/content/sfs/manuals/cytoscan_assay_user_manual.pdf).

Answer Id: E13972

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Product FAQ

For the CytoScan™ Assay, what is the role of purified PCR product?

Answer

The purification process is required to remove all the non-amplified DNA after the PCR process.

Answer Id: E13973

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Product FAQ

Can StemPRO® hESC SFM be used to culture mouse ESC's?

Answer

No, we don't recommend StemPro™ hESC SFM for mouse cells because they showed poor growth and some differentiation. We recommend KnockOut™ DMEM and KnockOut™ SR for mouse ESC cultures (catalog numbers 10829-018 and 10828-028 respectively).

Answer Id: E5302

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Product FAQ

Can I stop after the PCR process and continue the next day with the purification step for the CytoScan™ Assay?

Answer

Yes, the purified PCR products can be stored at -15 to -25 degrees C, and one can continue with the purification step later.

Answer Id: E13974

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Product FAQ

How many single-nucleotide polymorphism (SNP) and copy number (CN) probes are included on the array?

Answer

CytoScan™ HD Array includes 750,000 genotype-able SNPs and 1.9 million non-polymorphic probes.

Answer Id: E13990

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Product FAQ

How do I convert the bead concentration from 1 mg beads/mL to actual bead numbers per milliliter?

Answer

This depends on the bead size, in general, for M-450 beads, 1 mg/mL beads is approximately equivalent to 1.3 X 10E7 beads/mL; for M-280 beads, 1 mg/mL beads is approximately equivalent to 6.7 X 10E7 beads/mL.

Answer Id: E12125

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Product FAQ

How do I prepare the StemPRO® hESC SFM complete medium?

Answer

We recommend to thaw the supplement in the 37°C water bath, aliquot and freeze at -5°C to -20°C. Thaw each aliquot only one additional time. To make 100 ml complete medium, mix 90.8 ml DMEM/F-12 with GlutaMax™ matrix, 2 ml StemPRO™ hESC supplement, 7.2 ml 25% BSA solution, 80 ul bFGF (at 10 ug/ml), and 182 ul 2-mercaptoethanol (at 55mM). Store at 2°C-8°C until ready to use, but use the same day.

Answer Id: E5304

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Product FAQ

Do you offer stand alone replacement buffers for your specialized RNA purification kits?

Answer

Here (http://www.thermofisher.com/search/global/searchAction.action?query=dna%E2%81%84rna%20purification%20replacement%20buffers&resultsPerPage=15&personaFilterTerm=Catalog&show_seproductcategorynavigator=true&show_productcategorynavigator=true&show_taxonomynavigator=true&show_brandnavigator=true&firstSearch=true&mode=and&navigator=seproductcategorynavigator&modifier=General+Buffers%2FDNA%26frasl%3BRNA+Purification+Replacement+Buffers&ICID=ta-cat-dna%E2%81%84rna%20purification%20replacement%20buffers-DNA%E2%81%84RNA%20Purification%20Replacement%20Buffers) is a list of DNA/RNA Purification replacement buffers we offer as standalone.

Answer Id: E10246

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Product FAQ

How long can the purified PCR products be stored at -20 degrees C?

Answer

The purified PCR products can be stored at -15 to -25 degrees C for 10 days.

Answer Id: E13975

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Product FAQ

How long does CytoScan™ HD Array take to scan?

Answer

Each array takes ~32 minutes to scan.

Answer Id: E13991

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Product FAQ

Do you have any data on endogenous mutations in the GeneArt™ Engineered cell lines?

Answer

We have some genomic/transcriptomic characterization available that can be provided upon request. We guarantee only the genomic validation we provide in the datasheet/COA.

Answer Id: E10480

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Product FAQ

Do you have any alternatives for discontinued Dynabeads™ M-500 Subcellular?

Answer

The M-450 Tosylactivated beads (Cat. No. 14013) are very similar in terms of binding chemistry, concentration, and coupling protocol to the discontinued product Dynabeads™ M-500 Subcellular.

Answer Id: E12126

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Product FAQ

What is the amount of protein in BenchMark™ Pre-Stained Ladders?

Answer

BenchMark™ Pre-Stained Ladder has approximately 0.1 ug/uL protein per band.

Answer Id: E5330

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Product FAQ

What are the main characteristics of the Invitrogen™ Qubit™ Fluorometer?

Answer

The Qubit Fluorometer quantifies DNA, RNA, and protein with superior accuracy, sensitivity, and simplicity. It is designed for molecular biology labs working with precious samples that are rare or difficult to process, and applications requiring precise measurement such as real-time PCR. See more about the Qubit Fluorometer (http://www.thermofisher.com/us/en/home/life-science/laboratory-instruments/fluorometers/qubit/qubit-fluorometer.html.html).

Answer Id: E10247

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