Can I repair my Qubit™ 2.0 Fluorometer?

Product FAQ

Answer

No. The warranty will be voided if the instrument is disassembled or if you attempt to repair the instrument. It is best to call or email our technical support team for assistance.

Answer Id: E8124

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What if my Qubit™ 2.0 Fluorometer fails under warranty?

Product FAQ

Answer

We will replace your Qubit™ 2.0 Fluorometer. Please contact our Technical Support department for details by emailing techsupport@thermofisher.com.

Answer Id: E8125

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What kind of tubes do I need to buy for the Qubit™ 2.0 Fluorometer?

Product FAQ

Answer

Use thin-wall, clear 0.5 mL PCR tubes such as Qubit™ Assay Tubes (Cat. No. Q32856).

Answer Id: E8126

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What type of USB stick is compatible with the Qubit™ 2.0 Fluorometer?

Product FAQ

Answer

The one that comes with the Qubit™ Fluorometer is 1G, but the following types are also compatible:

  • ADISK USB 1.1 32MB flash disk
  • Aigo USB 1.1 64M flash disk
  • Crucial 1G flash disk
  • FPT-D US5B2H01 18-in-1 USB card reader/writer
  • HP 1G flash disk
  • IBM Portable Diskette Drive (floppy drive)
  • Integral USB 2.0 2GB flash disk
  • Kingston DataTraveler 1GB flash disk
  • Kingston DataTraveler 100 2GB flash disk
  • Kingston DataTraveler 16GB flash disk
  • LACIE USB 2.0 40GB mobile hard drive
  • Lexar Media JumpDrive Secure USB 2.0 512MB flash disk
  • Memorex 2GB flash disk
  • NCP XDrivePlus MMC/SD reader
  • Newman USB 1.1 64MB flash disk
  • PNY Attache USB 1.1 64MB flash disk
  • PNY Attache (U3) 1GB
  • PNY Attache 2GB
  • PNY Attache 8GB
  • PQI MMC/SD reader
  • RedLeaf USB 2.0 256MB flash disk
  • SanDisk Cruzer USB 2.0 256MB flash disk
  • SanDisk Cruzer Micro 2GB flash disk
  • SanDisk Cruzer Micro (U3) 2GB flash disk
  • SanDisk Cruzer Micro (U3) 4GB flash disk
  • SONY MICROVAULT USM256U2 USB 2.0 256MB flash disk
  • Transcend JF V30 4GB flash disk The following have not been verified by our R&D staff, but have been tested by customers:
  • Edge DiskGO™ 1GB USB Flash Drive Enhanced for ReadyBoost™
  • Edge DiskGO™ 2GB USB Flash Drive Enhanced for ReadyBoost™
  • Imation 1GB Swivel USB Flash Drive
  • Imation 2GB Swivel USB Flash Drive
  • Integral 1GB USB Memory Stick
  • MARKEM 1GB USB Memory Stick
  • Memorex 1GB TravelDrive™ USB Flash Drive
  • Memorex 2GB TravelDrive™ USB Flash Drive
  • PNY 1GB Attache USB Flash Drive
  • SanDisk 2GB Cruzer™ Crossfire USB Flash Drive
  • SanDisk 512MB Cruzer™ Micro USB Flash Drive
  • SanDisk 2GB Cruzer™ Micro USB Flash Drive
  • SanDisk 4GB Cruzer™ Micro USB Flash Drive (U3 function not initialized)
  • Sony 512MB Micro Vault Tiny USB Flash Drive
  • Sony 2GB Micro Vault Tiny USB Flash Drive
  • Sony 1GB Micro Vault Classic USB Flash Drive
  • Sony 4GB Micro Vault Classic USB Flash Drive
  • X Digital Media 1GB Itty Bit USB Flash Drive
  • X Digital Media 1GB Poker Chip USB Flash Drive
  • X Digital Media 2GB Itty Bit USB Flash Drive

    Answer Id: E8127

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  • The Qubit™ Fluorometer screen indicates my samples are OUT OF RANGE (too high). What can I do?

    Product FAQ

    Answer

    Dilute your sample, or use a smaller volume of sample in the assay and try again. Alternatively, if you are using the Qubit™ DNA HS Assay or the Qubit™ RNA HS Assay, try using the Qubit™ DNA BR Assay or Qubit™ RNA BR Assay, as these have lower sensitivity.

    Answer Id: E8144

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    What are the excitation/emission wavelengths for dyes in the Qubit™ Assays?

    Product FAQ

    Answer

  • Qubit™ dsDNA HS Assay: 502 nm/532 nm
  • Qubit™ dsDNA BR Assay: 510 nm/527 nm
  • Qubit™ ssDNA Assay: 493 nm/518 nm
  • Qubit™ RNA HS Assay: 644 nm/673 nm
  • Qubit™ RNA BR Assay: 644 nm/673 nm
  • Qubit™ microRNA Assay: 498 nm/518 nm
  • Qubit™ Protein Assay: 470 nm/570 nm

    Answer Id: E8128

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  • The Qubit™ Fluorometer screen indicates my samples are OUT OF RANGE (too low). What can I do?

    Product FAQ

    Answer

    You can try to use a larger amount of sample if possible (up to 20 μL). Alternatively, if you are using the Qubit™ DNA BR Assay or Qubit™ RNA BR Assay, try using the Qubit™ DNA HS Assay or Qubit™ RNA HS Assay, as these have higher sensitivity.

    Answer Id: E8145

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    How sensitive is SuperSignal ELISA Pico Chemiluminescent Substrate?

    Product FAQ

    Answer

    The substrate allows measurement of low picogram levels in sandwich ELISA procedures.

    Answer Id: E8491

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    What is the difference between the Quant-iT™ PicoGreen™ DNA, Quant-iT™ DNA, and Qubit™ DNA assays?

    Product FAQ

    Answer

    The Quant-iT™ PicoGreen™ DNA assay is the oldest assay and the most general-purpose. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. The Qubit™ assays are intended for low throughput (<20 samples), and are only used on the Qubit™ Fluorometer. The Qubit™ Fluorometer contains highly optimized algorithms that calculate the concentration of your sample for you. The performance of all of these assays is similar.

    Answer Id: E8129

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    My Qubit™ Fluorometer quantification value is lower than the UV absorbance value. What is causing this?

    Product FAQ

    Answer

    Most likely, the sample is contaminated with other molecules that absorb at 260 nm. The NanoDrop™ Spectrophotometer is reading these contaminants, but the Qubit™ Fluorometer is not. To determine the exact composition of the sample, perform a Qubit™ dsDNA Assay, Qubit™ RNA HS Assay, and Qubit™ Protein Assay on small aliquots of the same sample. Check the Qubit™ assay kit product manual for a list of contaminants that could interfere with the assay. Dilute or purify the sample further to reduce contaminant concentration. Mix the working solution and the sample aliquoted into it well.

    Answer Id: E8146

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    What signal duration occurs with SuperSignal ELISA Pico Chemiluminescent Substrate?

    Product FAQ

    Answer

    Maximum signal is reached within five minutes and then declines slowly thereafter until it is much less after an hour.

    Answer Id: E8492

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    I want to adapt my Quant-iT™ PicoGreen™ DNA kit to a 96-well plate format. Is this possible?

    Product FAQ

    Answer

    Yes, we would recommend reducing the volume while keeping the dye: sample ratio constant.

    Answer Id: E8130

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    Will my protein be in its native conformation after B-PER Reagent extraction?

    Product FAQ

    Answer

    While this is protein-dependent, many proteins have been successfully tested in downstream applications (reporter assays, immunoprecipitation and beta-Gal assays), including GST and 6xHis proteins. Otherwise, samples can be diluted or dialyzed to remove any interfering substances.

    Answer Id: E8164

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    The value is decreasing over time when using the Qubit™ Fluorometer. What could be causing this?

    Product FAQ

    Answer

    Please see our suggestions below:

  • Make sure that you take your reading only after incubating for 2 minutes (15 minutes for protein).
  • If you leave the assay tube in the Qubit™ Fluorometer and take multiple readings, the readings will go down as the tube heats up inside the instrument. If you want to take multiple readings, remove the tube from the instrument, place it in a tube rack, and allow it to equilibrate to room temperature for at least 30 seconds before rereading the tube.
  • You may read the sample up to 3 hours after mixing if it is protected from light. After this time, the reading will not be accurate.
  • Keep standards and sample tubes in the dark and protected from light in between readings.

    Answer Id: E8147

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  • Is there a potential for gene silencing when using your algae kits?

    Product FAQ

    Answer

    Yes, unfortunately, silencing is a big problem in algae. The extent of silencing depends on the sequence of the gene and the toxicity of the protein that expresses from that gene to the cell.
    The engineering kit is designed for random insertion of your gene of interest, which can lead to your gene being lost quickly. The protein expression kit, which contains the pChlamy_4 vector, is designed so that proteins are expressed as transcriptional fusions with the bleomycin/zeocin resistance gene sh-ble (Rasala et al., 2012). The self-cleaving sequence for the 2A peptide from the foot-and-mouth disease virus (FMDV) is placed between the antibiotic resistance gene and the gene of interest. It encodes a short ~20 amino acid sequence that mediates proper cleavage to yield two discrete proteins. With this system we have seen positive transformants maintain high expression levels for much longer than with other systems, even after many passages with or without selection pressure.

    Answer Id: E9567

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