Product FAQ

Which GCOS features are not supported in Command Console Software?

Answer

Publishing and publish databases will not be supported. However, customers can leverage the flexibility of the file-based system to populate their own library information management systems (LIMS).
CHP data will be generated by separate Affymetrix™ and third-party applications, including Expression Console Software and Genotyping Console Software.
GeneArray 2500 and Fluidics Station FS400 instruments are not supported by Command Console Software.
Windows 2000 operating system is not supported.

Answer Id: E14228

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What is the difference between the pCR2.1-TOPO™ vector and the pCRII-TOPO™ vector?

Answer

The pCRII-TOPO™ vector is a dual promoter vector. It contains a T7 promoter at the 5' end of the multiple cloning site and an Sp6 promoter at the 3' end. The pCR2.1-TOPO™ vector only contains the T7 promoter at the 5' end. The dual promoter vector is only of advantage for in vitro transcription studies with the cloned insert. In terms of cloning efficiency, there is no difference between the pCR2.1-TOPO™ and the pCRII-TOPO™ plasmids. Both the pCR2.1-TOPO™ and the pCRII-TOPO™ vector contain M13 sequencing primer sites to sequence the cloned insert in both directions.

Answer Id: E3330

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Are the Affymetrix™ Analysis Data Model (AADM) and Data Mining Tool (DMT) supported by Command Console Software?

Answer

AADM is a relational database model and is not supported by Command Console Software. Customers can access the Command Console indexer to get query capability. DMT is not supported by Command Console. Analysis capabilities similar to those in DMT are offered by various Affymetrix™ packages and by third-party GeneChip-Compatible (http://www.thermofisher.com/us/en/home/life-science/microarray-analysis/microarray-analysis-partners-programs/genechip-compatible-software-providers.html) packages.

Answer Id: E14229

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What concentration of insert and vector do you recommend for most ligation reactions?

Answer

Recommendations would vary depending on the size of the vector and insert or the nature of the insert, but for most plasmid cloning or subcloning reactions, a vector concentration of 1-10 ng/µl is recommended. Inserts should generally be 2- to 3-fold excess in molar concentration relative to the vector.

Answer Id: E4013

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Are the TA or TOPO™ TA Cloning™ vectors available to purchase without competent cells? What about your Zero Background™, Zero Blunt™, or Zero Blunt™ TOPO™ Cloning Kits

Answer

Most of our cloning vectors are offered in a complete format, which includes competent cells. While in most cases other cells can be used with our vectors, we cannot guarantee the results you will get with our cloning vectors if you use your own competent cells. For this reason, most TOPO™ TA Cloning™ vectors can only purchased in a complete kit with cells, although there are a few exceptions. Non-TOPO™ vectors are generally available in multiple formats, with and without cells. To get the most current information on available products, visit the Cloning section of our website under Products & Service.

Zero Background™ and Zero Blunt™ vectors are available without competent cells provided, but you should especially careful in choosing competent cells to use with them. These vectors contain the ccdB gene for efficient negative selection of clones without insert, and some E. coli strains are not compatible with the mechanism of negative selection by the lethal activity of the ccdB gene product. In particular, cells with the F' episome have a ccd locus containing the ccdA gene, which prevents ccdB protein cell-killing. Therefore, cells without the F' episome are recommended so that only the CcdB protein will be expressed, and its cell-killing ability will not be inhibited or reduced by CcdA.

Answer Id: E3331

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Where were Gibco™ Rat Primary Cortical Astrocytes (Cat. No. N7745) isolated from? How many passages do these cells go through before being frozen down?

Answer

Rat cortical astrocytes were isolated from the cortex of fetal (E-19) Sprague-Dawley rats. The cells were cryopreserved in growth medium supplemented with 10% DMSO at passage 1.

Answer Id: E12007

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What happens to GCOS if Command Console Software is installed on the same workstation?

Answer

GCOS is not removed or updated during the install of the Command Console Software. GCOS and the Command Console Software can be installed on the same computer workstation. However, GCOS and Command Console Software cannot control the same instrumentation at the same time.

Answer Id: E14230

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Plate warping is causing problems with the robot in my automated processes. How can I solve this?

Answer

We recommend Thermo Scientific™ Armadillo PCR plates which are designed for robotic handling with high rigidity and resistance. Combining the rigidity of a polycarbonate frame with thin walled polypropylene wells, the Armadillo plates provide superior thermal cycling performance under all conditions without warping.

Answer Id: E8883

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

What instruments can the dGTP BigDye™ Terminator Cycle Sequencing Kit be run on?

Answer

The dGTP BigDye™ Terminator Cycle Sequencing Kit can be run on all of our capillary electrophoresis platforms:

- Applied Biosystems™ 3500/3500xL Genetic Analyzers
- Applied Biosystems™ 3730/3730xl DNA Analyzers
- Applied Biosystems™ 310 Genetic Analyzer
- Applied Biosystems™ 3130/3130xl DNA Analyzers

However, due to the nature of the chemistry, compressions in the data are expected.

Answer Id: E15352

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

If the plates are stored overnight at 4 degrees C after Day 1 procedures, should we spin the plates first before continuing with the PrimeFlow™ RNA assay?

Answer

Normally, spinning is not required. If there is significant condensation after overnight storage, spin plate at 400 xg for 1 minute before proceeding.

Answer Id: E14495

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Do both my insert and vector need to be phosphorylated for ligation?

Answer

At least one molecule in a ligase reaction (i.e., insert or vector) must be phosphorylated. Ligation reactions are dependent on the presence of a 5' phosphate on the DNA molecules. The ligation of a dephosphorylated vector with an insert generated from a restriction enzyme digest (phosphorylated) is most routinely performed. Although only one strand of the DNA ligates at a junction point, the molecule can form a stable circle, providing that the insert is large enough for hybridization to maintain the molecule in a circular form.

Answer Id: E4014

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Do PCR products produced with Stoffel fragment have sufficient A-overhangs to work with TA Cloning™ kit?

Answer

Yes. Stoffel fragment is a 61 kDa modified form of AmpliTaq™ DNA Polymerase that lacks 5' to 3' exonuclease activity. It is more thermostable than AmpliTaq™ DNA Polymerase, giving it improved performance at the higher denaturating temperatures used with templates that have secondary structure. It more active over a wider range of magnesium concentrations (2 mM to 10 mM MgCl2) than Taq polymerase.

Answer Id: E3332

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Can I use the iBlot™ Filter Paper for blotting E-PAGE™ gels?

Answer

We do not recommend using the iBlot™ Filter Paper for blotting E-PAGE™ gels.

Answer Id: E11350

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

How long can I culture Gibco™ Rat Primary Cortical Astrocytes?

Answer

Rat cortical astrocytes can be expanded in culture for at least one passage. The recommended medium can provide 3-15 fold increase in cell number over 10 days. For consistent results in your experiments, we recommend using cells below passage 3 (P3). If you expand these cells beyond P3, we recommend that you perform another round of characterization prior to further experiments.

Answer Id: E12008

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Can DAT image files be viewed while the array scan is in progress?

Answer

No. DAT files are available for viewing after the scan has completed.

Answer Id: E14248

Was this answer helpful?

Yes
No
Thank you for your response