Precise and flexible editing, targeting any gene in any cell with all-around freedom

We’ve developed robust algorithms that allow us to generate the most optimal TAL effector designs. Your choice of targets is practically unlimited. The predictability with which Invitrogen™ GeneArt™ TAL effectors bind to exact DNA sequences makes it possible to target any sequence in the genome. From designing, synthesizing and validating your TALEN, we offer end-to-end services to support every step in genome editing. Our dedicated staff of R&D scientists and technical support specialists are ready to partner with you and your colleagues to accelerate your research program.

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Licensing TALEN technology

Thermo Fisher Scientific is currently the only provider of TALEN™ technology products and services that includes rights under foundational TAL IP invented at Martin-Luther-University Halle-Wittenberg, the University of Minnesota, and Iowa State University. For further information on licensing our TALEN products/services for use outside of internal research please contact us at outlicensing@thermofisher.com

Available designer TALEN engineering services

The choice of the effector domain determines whether the TAL effector edits, activates, or represses the targeted gene. GeneArt TAL effectors are supplied as either lyophilized DNA in the form of Invitrogen™ Gateway™ entry clones, or as mRNA. Choose from a number of services to customize your genome engineering project. See also custom TALEN cell line engineering services.

 
Available format TAL FOKI Endonuclease TAL VP64 Activator TAL KRAB Repressor TAL-MCS
Recommended format based on application KO, deletion, and KI Activate endogenous gene expression Repress endogenous gene expression Customize the TAL fusion protein with an effector domain of your choice
Deliverable format

Plasmid DNA or mRNA

Plasmid DNA or mRNA  Plasmid DNA or mRNA  Plasmid DNA
Compatible transfection reagent TAL plasmid- Lipofectamine™ 3000 or Neon™ TAL mRNA- MessengerMax™ or Neon TAL plasmid- Lipofectamine 3000 or Neon TAL mRNA- MessengerMax or Neon  TAL plasmid- Lipofectamine 3000 or Neon TAL mRNA- MessengerMax or Neon TAL plasmid- Lipofectamine 3000 or Neon

Based on our experience, we recommend that you design and test at least 3 TAL effector pairs and choose the design that provides you with the highest efficiency and minimal off-target effects. We therefore offer a validation service where we design and test at least 3 sequence variations and provide you with the best performing TAL pair.

Tool validation services

  • Validate TALEN efficacy via GCD assay
  • Validate TALEN efficacy via on-target NGS analysis

TALEN activator & repressor validation services

  • Validate TALEN efficacy via GCD assay
  • Validate TALEN efficacy via on-target NGS analysis
  • Taqman gene expression qPCR analysis

Let’s work together to get your genome engineering project started

Discover how our off-the-shelf product portfolio and outsourced services can meet your needs. Contact our dedicated technical support team with any questions, or get started on your services project today:

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