Seminar
Jul 29, 2018 - Aug 02, 2018
Washington Marriott Wardman Park | 2660 Woodley Rd NW - Washington, D.C.
HPLC 2018

Join Thermo Fisher Scientific at HPLC 2018 in Washington DC. Visit us at Booth 108 to discover the many exciting new developments and applications we have on display. Interact with our experts during our lunchtime seminars and scientific presentations and be sure to discover our range of informative posters.

Lunchtime seminars

Effectively Supporting Synthetic Chemistry for Pharmaceutical and Academic Research

Monday, July 30, 12:25 – 1:25 p.m.
Jenny Wong, HPLC Product Marketing Specialist, Thermo Fisher Scientific

Monitoring the synthesis of small or larger molecules is a key activity in pharmaceutical but also academic research. The investigation of new structural entities and compounds often requires re-creation of the characterized novel structures to conduct further general research or identify therapeutic properties. This workshop will showcase our comprehensive new workflow solution discussing not only fast mass confirmation, but also how to produce better mass balances of unknown impurities and non-chromophores. In addition, we discuss advanced system configurations multiplying detection options as well as productivity of multi-detector UHPLC setups.

Register

Simple Approaches to Charge Variant Analysis

Tuesday, July 31, 12:25 – 1:25 p.m.
Jonathan Bones, Principal Investigator, NIBRT Characterization and Comparability Laboratory, NIBRT

The characterization of monoclonal antibodies (mAbs) during biopharmaceutical development involves the identification, monitoring, and analysis of charge variants. Antibodies can exhibit changes in charge heterogeneity during production and purification caused by amino acid substitutions, glycosylation, and other post-translational or chemical modifications. Not only can these changes impact stability and activity, they can also cause adverse immunological reactions. Identification of charge variants in development, and their monitoring throughout manufacturing is therefore critical. In this presentation the use of ion exchange and reverse phase approaches is discussed along with enabling technologies that simplify analysis.

Register

Oral presentations

Tuesday
1:30 p.m. (L-092)
Parallel Session: Glycomics – I

Keynote Lecture: High pH Anion Exchange Separation of Carbohydrates: Past, Present and Future
Christopher Pohl, Thermo Fisher Scientific (recipient of the 2018 Uwe D. Neue Award in Separation Science)

2:35 p.m. (L-091)
Parallel Session: Quality by Design

Algorithmic Approach to LC-MS and IC-MS Method Development and Optimization of the ESI Ion Source Settings
Alexander Semyonov, Thermo Fisher Scientific

Wednesday
11:45 a.m. (L-132)

Parallel Session: Advances in Liquid Chromatography – III
Complementary Parallel LC as a Convenient Alternative to Multi Heart-Cut LC for Samples of Medium Complexity
Maria Gruebner, Mauro De Pra, Frank Steiner, Thermo Fisher Scientific

Posters

Poster Session 1
Monday, 10:00 – 11:15 a.m.
Exhibition Hall C

P-M-0100 – Sample Preparation

  • P-M-0111: A Fast and Simple Workflow for Surrogate Peptide Bioanalysis: NISTmAb Case Study

P-M-0300 – Characterization of Monoclonal Antibodies/Drug Conjugates/Protein-based Drugs

  • P-M-0301: Charge Variant Analysis of Therapeutic Proteins using a Novel Weak Cation Exchange Stationary Phase
  • P-M-0308: Development of NISTmAb-derived Homogeneous Antibody-drug Conjugate (ADC) Standards
  • P-M-0318: Accurate and Precise Quantification of mAb-released N-glycans with an Amide HILIC Column
  • P-M-0319: Charge Variant Method Design for Analysis of Monoclonal Antibodies
  • P-M-0320: High Throughput, Flexible Chromatographic Analysis of Monoclonal Antibodies

Poster Session 2
Monday, 2:50 – 4:30 p.m.
Exhibition Hall C

P-M-0600 – Foods/Beverages and Nutrition

  • P-M-0606: Analysis of Carbohydrates in Beer using Liquid Chromatography Triple Quadrupole Mass Spectrometry
  • P-M-0607: Determination of Four Aflatoxins in Hazelnuts by Immunoaffinity-SPE with HPLC-FLD Detection without Photo Derivatization

Poster Session 3
Tuesday, 10:00 – 11:15 a.m.
Exhibition Hall C

P-T-0800 – Protein Characterization and Modification

  • P-T-0811: Tandem UHPLC Operation for High-throughput LC-MS Peptide Mapping Analyses
  • P-T-0813: High-precision, Automated Peptide Mapping of Proteins

P-T-0900 – Omics (metabolomics/lipidomics/glycomics/proteomics/genomics)

  • P-T-0913: Capillary-flow LC-MS Platform for Robust and Sensitive High-throughput Proteomics
  • P-T-0916: Continuous MS Utilization for Proteomics Data Acquisition using a Novel Low-flow Tandem LC-MS Setup

P-T-1000 – Method Development and Automation

  • P-T-1016: Modernization of USP Methods using Ion Chromatography (IC) for Active Pharmaceutical Ingredient (API) Determination

Poster Session 4
Tuesday, 2:50 – 4:30 p.m.
Exhibition Hall C

P-T-1400 – Instrument Design and Applications

  • P-T-1409: HPAE-PAD Analysis of N-linked Oligosaccharides from Glycoproteins using Dual Eluent Generation Cartridge Mode
  • P-T-1411: Use of a Novel UHPLC System for the Simultaneous UHPLC Analysis of Water-soluble and Fat-soluble Vitamins
  • P-T-1413: Fatty Acid Analysis in Polysorbate 80 by UHPLC-CAD
  • P-T-1414: A Multi-detector Set-up Comprising UV/Vis, Charged Aerosol and Single Quadrupole Mass Spectrometric Detection for Comprehensive Quantitative Sample Analysis

Poster Session 5
Wednesday, 10:00 – 11:15 a.m.
Exhibition Hall C

P-W-1600 – Forensics/Toxicology/Drugs of Abuse

  • P-W-1609: Tailoring Solvent Purity for Liquid Phase Separation Analysis

P-W-1700 – Biopharmaceutical and Pharmaceutical Applications

  • P-W-1712: Determination of Gentamicin and Related Impurities in Gentamicin Sulfate
  • P-W-1716: Full Characterization and Confirmation of Diverse Oligonucleotides by Ion Pair LC-MS/MS

Products

Three workflows. Two flow paths. One integrated UHPLC solution. Improve your productivity and sample knowledge for a faster return on investment.

Resources


The Q Exactive HF-X MS rises to the occasion and sets new standards in sensitivity, performance, and productivity.

Resources


Allows any chromatographer to easily access mass spectrometry with a system that has unmatched low mass sensitivity, is simple-to-use and robust.

Resources


Separate your science from the status quo. Experience uncompromised UHPLC—with no trade-offs in performance, robustness and ease of use.

Resources


Make the invisible visible with a versatile, universal detector that adds a unique form of detection to the speed and resolution of your UPHLC system.

Resources


With the ability to use 4-μm particle size IC columns on high-pressure capable IC systems, you can finally choose between achieving even faster run times or better chromatographic resolution.

Resources


The Thermo Scientific nano and capillary systems are ideal for use as an integrated part of the proteomics workflow and seamlessly combine with our mass spectrometry systems

Resources


Our extensive family of products includes a variety of particle sizes and column designs to meet all separation needs, offering improved resolution, enhanced sensitivity, faster analysis and consistent performance.

Resources


From microsampling to headspace applications, you'll find the right vials and closures to match your application and autosampler requirements from our comprehensive portfolio.

Resources


Simplify your lab workflow with unified control, processing and data management for your chromatography/mass spectrometry instruments.

Resources


For all HPLC/UHPLC and LC-MS applications, each Fisher Chemical high-quality solvent undergoes rigorous quality assurance and testing measures.


Deliver robust reproducible gradients with the Thermo Scientific pH buffer platform that is applicable to a wide range of mAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

Lunchtime seminars

Effectively Supporting Synthetic Chemistry for Pharmaceutical and Academic Research

Monday, July 30, 12:25 – 1:25 p.m.
Jenny Wong, HPLC Product Marketing Specialist, Thermo Fisher Scientific

Monitoring the synthesis of small or larger molecules is a key activity in pharmaceutical but also academic research. The investigation of new structural entities and compounds often requires re-creation of the characterized novel structures to conduct further general research or identify therapeutic properties. This workshop will showcase our comprehensive new workflow solution discussing not only fast mass confirmation, but also how to produce better mass balances of unknown impurities and non-chromophores. In addition, we discuss advanced system configurations multiplying detection options as well as productivity of multi-detector UHPLC setups.

Register

Simple Approaches to Charge Variant Analysis

Tuesday, July 31, 12:25 – 1:25 p.m.
Jonathan Bones, Principal Investigator, NIBRT Characterization and Comparability Laboratory, NIBRT

The characterization of monoclonal antibodies (mAbs) during biopharmaceutical development involves the identification, monitoring, and analysis of charge variants. Antibodies can exhibit changes in charge heterogeneity during production and purification caused by amino acid substitutions, glycosylation, and other post-translational or chemical modifications. Not only can these changes impact stability and activity, they can also cause adverse immunological reactions. Identification of charge variants in development, and their monitoring throughout manufacturing is therefore critical. In this presentation the use of ion exchange and reverse phase approaches is discussed along with enabling technologies that simplify analysis.

Register

Oral presentations

Tuesday
1:30 p.m. (L-092)
Parallel Session: Glycomics – I

Keynote Lecture: High pH Anion Exchange Separation of Carbohydrates: Past, Present and Future
Christopher Pohl, Thermo Fisher Scientific (recipient of the 2018 Uwe D. Neue Award in Separation Science)

2:35 p.m. (L-091)
Parallel Session: Quality by Design

Algorithmic Approach to LC-MS and IC-MS Method Development and Optimization of the ESI Ion Source Settings
Alexander Semyonov, Thermo Fisher Scientific

Wednesday
11:45 a.m. (L-132)

Parallel Session: Advances in Liquid Chromatography – III
Complementary Parallel LC as a Convenient Alternative to Multi Heart-Cut LC for Samples of Medium Complexity
Maria Gruebner, Mauro De Pra, Frank Steiner, Thermo Fisher Scientific

Posters

Poster Session 1
Monday, 10:00 – 11:15 a.m.
Exhibition Hall C

P-M-0100 – Sample Preparation

  • P-M-0111: A Fast and Simple Workflow for Surrogate Peptide Bioanalysis: NISTmAb Case Study

P-M-0300 – Characterization of Monoclonal Antibodies/Drug Conjugates/Protein-based Drugs

  • P-M-0301: Charge Variant Analysis of Therapeutic Proteins using a Novel Weak Cation Exchange Stationary Phase
  • P-M-0308: Development of NISTmAb-derived Homogeneous Antibody-drug Conjugate (ADC) Standards
  • P-M-0318: Accurate and Precise Quantification of mAb-released N-glycans with an Amide HILIC Column
  • P-M-0319: Charge Variant Method Design for Analysis of Monoclonal Antibodies
  • P-M-0320: High Throughput, Flexible Chromatographic Analysis of Monoclonal Antibodies

Poster Session 2
Monday, 2:50 – 4:30 p.m.
Exhibition Hall C

P-M-0600 – Foods/Beverages and Nutrition

  • P-M-0606: Analysis of Carbohydrates in Beer using Liquid Chromatography Triple Quadrupole Mass Spectrometry
  • P-M-0607: Determination of Four Aflatoxins in Hazelnuts by Immunoaffinity-SPE with HPLC-FLD Detection without Photo Derivatization

Poster Session 3
Tuesday, 10:00 – 11:15 a.m.
Exhibition Hall C

P-T-0800 – Protein Characterization and Modification

  • P-T-0811: Tandem UHPLC Operation for High-throughput LC-MS Peptide Mapping Analyses
  • P-T-0813: High-precision, Automated Peptide Mapping of Proteins

P-T-0900 – Omics (metabolomics/lipidomics/glycomics/proteomics/genomics)

  • P-T-0913: Capillary-flow LC-MS Platform for Robust and Sensitive High-throughput Proteomics
  • P-T-0916: Continuous MS Utilization for Proteomics Data Acquisition using a Novel Low-flow Tandem LC-MS Setup

P-T-1000 – Method Development and Automation

  • P-T-1016: Modernization of USP Methods using Ion Chromatography (IC) for Active Pharmaceutical Ingredient (API) Determination

Poster Session 4
Tuesday, 2:50 – 4:30 p.m.
Exhibition Hall C

P-T-1400 – Instrument Design and Applications

  • P-T-1409: HPAE-PAD Analysis of N-linked Oligosaccharides from Glycoproteins using Dual Eluent Generation Cartridge Mode
  • P-T-1411: Use of a Novel UHPLC System for the Simultaneous UHPLC Analysis of Water-soluble and Fat-soluble Vitamins
  • P-T-1413: Fatty Acid Analysis in Polysorbate 80 by UHPLC-CAD
  • P-T-1414: A Multi-detector Set-up Comprising UV/Vis, Charged Aerosol and Single Quadrupole Mass Spectrometric Detection for Comprehensive Quantitative Sample Analysis

Poster Session 5
Wednesday, 10:00 – 11:15 a.m.
Exhibition Hall C

P-W-1600 – Forensics/Toxicology/Drugs of Abuse

  • P-W-1609: Tailoring Solvent Purity for Liquid Phase Separation Analysis

P-W-1700 – Biopharmaceutical and Pharmaceutical Applications

  • P-W-1712: Determination of Gentamicin and Related Impurities in Gentamicin Sulfate
  • P-W-1716: Full Characterization and Confirmation of Diverse Oligonucleotides by Ion Pair LC-MS/MS

Products

Three workflows. Two flow paths. One integrated UHPLC solution. Improve your productivity and sample knowledge for a faster return on investment.

Resources


The Q Exactive HF-X MS rises to the occasion and sets new standards in sensitivity, performance, and productivity.

Resources


Allows any chromatographer to easily access mass spectrometry with a system that has unmatched low mass sensitivity, is simple-to-use and robust.

Resources


Separate your science from the status quo. Experience uncompromised UHPLC—with no trade-offs in performance, robustness and ease of use.

Resources


Make the invisible visible with a versatile, universal detector that adds a unique form of detection to the speed and resolution of your UPHLC system.

Resources


With the ability to use 4-μm particle size IC columns on high-pressure capable IC systems, you can finally choose between achieving even faster run times or better chromatographic resolution.

Resources


The Thermo Scientific nano and capillary systems are ideal for use as an integrated part of the proteomics workflow and seamlessly combine with our mass spectrometry systems

Resources


Our extensive family of products includes a variety of particle sizes and column designs to meet all separation needs, offering improved resolution, enhanced sensitivity, faster analysis and consistent performance.

Resources


From microsampling to headspace applications, you'll find the right vials and closures to match your application and autosampler requirements from our comprehensive portfolio.

Resources


Simplify your lab workflow with unified control, processing and data management for your chromatography/mass spectrometry instruments.

Resources


For all HPLC/UHPLC and LC-MS applications, each Fisher Chemical high-quality solvent undergoes rigorous quality assurance and testing measures.


Deliver robust reproducible gradients with the Thermo Scientific pH buffer platform that is applicable to a wide range of mAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

Would you like to speak with an expert?

Yes, I would like to speak with an expert to learn how Thermo Scientific products can help maximize productivity in my laboratory.

Contact us