Gibco® B-27® Supplement

For nearly 20 years, researchers have been publishing their experiments using B-27® supplements. The first commercially available, serum-free supplement for neural cell culture, B-27® Serum Free Supplement was developed for the long-term culture of rat hippocampal and cortical neurons. Since its introduction, the publication history of B-27® supplements demonstrates that researchers are expanding the use of these supplements far beyond these original applications.

Read how a University of Wisconsin-Madison team used B-27® Minus Insulin in their cardiomyocyte differentiation study.

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The most referenced neural cell culture supplement

Our exclusive Gibco® B-27® supplements are the most referenced neural cell culture supplements. B-27® supplements have been cited in thousands of peer-reviewed publications spanning a variety of different cell types, and used in the research of many different neurological disease models.

Which B-27® supplement is right for you?

To order the right B-27® supplement for you, click on the application/cell-type tab below and then click on the product name or catalog number to order.

All Gibco® neural cell culture reagents are optimized to work together as a complete media system. Need a complementary media or cells? Browse our neural cell culture product guide.

Standard Applications / Primary Neurons

Application Product name Catalog No.
Growth and maintenance of neuronsB-27® Serum-Free Supplement17504044

Stem cells and Precursor cells

Application Product name Catalog No. 
Proliferation of stem cellsB-27® Supplement, minus vitamin A12587010
Differentiation of stem cellsB-27® Supplement, XenoFree CTS™A14867-01


Specialty applications

Application Product name Catalog No.
Studies where XenoFree components are required B-27® Supplement, XenoFree CTS™ A14867-01
Studies of oxidative stress/damage, apoptosis,
or where free radical damage to neurons occurs
B-27® Supplement,
minus AO (antioxidants)
Studies of insulin secretion or insulin receptors B-27® Supplement,
minus Insulin
Electrophysiology studies B-27® Electrophysiology Kit A1413701
Growth and expansion of retinal ganglion cells B-27® Supplement, Custom 0080085SA

Table 1. Cell types and disease models cited in publications using Gibco® neural cell culture reagents

Primary cells

Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia nigral, sympathetic ganglia

Induced pluripotent stem cell, neural precursor, neural stem cell, neuroepithelial, cardiomyocytes
Astrocyte, microglia, oligodendrocytes, radial glia, satellite, schwann
Disease Models Alzheimer’s disease, amyotropyic lateral sclerosis (ALS), brain tumor, cerebral palsy, epilepsy, glioma, Huntington’s disease, multiple sclerosis, Parkinson’s disease, schizophrenia, spinal cord injury, stroke


For research use only. Not for human or animal therapeutic or diagnostic use.