For nearly 20 years, researchers have been publishing their experiments using Gibco B-27 cell culture supplements. The first commercially available, serum-free supplement for neural cell culture, Gibco B-27 Serum Free Supplement was developed for the long-term culture of rat hippocampal and cortical neurons. Since its introduction, the publication history of B-27 supplements demonstrates that researchers are expanding the use of these supplements far beyond their original applications.

The standard in neuronal cell culture just got better

The most-referenced neural cell culture supplement just got better

The desire for more reliable and biologically relevant models has increased, so too has the necessity for a next-generation media system that can maintain and mature optimal densities of functional neurons over longer periods of time in vitro. The new B-27 Plus Supplement is the next generation of the most-cited neuronal cell culture product, B-27 Supplement.

NEW The Gibco B-27 Plus Neuronal Culture System features an optimized formulation, upgraded manufacturing process, and more stringent quality control for raw materials and final product. 

These improvements enable:

  • Increased neuronal survival by more than 50%—the highest in the industry
  • Accelerated neurite outgrowth
  • Improved electrophysiological activity and maturation of neurons

Which B-27 supplement is right for you?

To order the right B-27 supplement for you, choose the application/cell-type tab below and then click on the product name or catalog number to order.

All Gibco neural cell culture reagents are optimized to work together as a complete media system. Need a complementary media or cells? Browse our neural cell culture product guide.

Standard Applications / Primary Neurons

Application Product name Cat. No.
Maintenance/maturation of prenatal/fetal primary neurons
B-27 Plus Supplement A3582801

Neural stem cells and progenitor cells

Application Product name Cat. No.
Proliferation of neural stem cells B-27 Supplement, minus vitamin A 12587010
Differentiation and maintenance/maturation of stem cell–derived neurons B-27 Plus Supplement A3582801
B-27 Supplement 17504044
Xeno-free proliferation of neural stem cells CTS B-27 Supplement, XenoFree minus Vitamin A A3353501
Xeno-free differentiation of neural stem cells CTS B-27 Supplement, XenoFree A1486701

 

Specialty applications

Application Product name Cat. No.
Studies where XenoFree components are required CTS B-27 Supplement, XenoFree A14867-01
Studies of oxidative stress/damage, apoptosis,
or where free radical damage to neurons occurs
B-27 Supplement,
minus AO (antioxidants)
10889038
Studies of insulin secretion or insulin receptors B-27 Supplement,
minus Insulin
A1895601
Electrophysiology studies B-27 Plus Neuronal Culture System A365401

Standard Applications / Primary Neurons

Application Product name Cat. No.
Maintenance/maturation of prenatal/fetal primary neurons
B-27 Plus Supplement A3582801

Neural stem cells and progenitor cells

Application Product name Cat. No.
Proliferation of neural stem cells B-27 Supplement, minus vitamin A 12587010
Differentiation and maintenance/maturation of stem cell–derived neurons B-27 Plus Supplement A3582801
B-27 Supplement 17504044
Xeno-free proliferation of neural stem cells CTS B-27 Supplement, XenoFree minus Vitamin A A3353501
Xeno-free differentiation of neural stem cells CTS B-27 Supplement, XenoFree A1486701

 

Specialty applications

Application Product name Cat. No.
Studies where XenoFree components are required CTS B-27 Supplement, XenoFree A14867-01
Studies of oxidative stress/damage, apoptosis,
or where free radical damage to neurons occurs
B-27 Supplement,
minus AO (antioxidants)
10889038
Studies of insulin secretion or insulin receptors B-27 Supplement,
minus Insulin
A1895601
Electrophysiology studies B-27 Plus Neuronal Culture System A365401

Table 1. Cell types and disease models cited in publications using Gibco neural cell culture reagents

Primary cells Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia nigral, sympathetic ganglia
Stem cells Induced pluripotent stem cell, neural precursor, neural stem cell, neuroepithelial, cardiomyocytes
Glial cells Astrocyte, microglia, oligodendrocytes, radial glia, satellite, schwann
Disease models Alzheimer’s disease, amyotropyic lateral sclerosis (ALS), brain tumor, cerebral palsy, epilepsy, glioma, Huntington’s disease, multiple sclerosis, Parkinson’s disease, schizophrenia, spinal cord injury, stroke

Neural cell culture resources