For nearly 20 years, researchers have been publishing their experiments using Gibco™ B-27™ cell culture supplements. The first commercially available, serum-free supplement for neural cell culture, Gibco B-27 Serum Free Supplement was developed for the long-term culture of rat hippocampal and cortical neurons. Since its introduction, the publication history of B-27 supplements demonstrates that researchers are expanding the use of these supplements far beyond their original applications.

The most referenced neural cell culture supplement

Our exclusive B-27 supplements are the most referenced neural cell culture supplements. B-27 supplements have been cited in thousands of peer-reviewed publications spanning a variety of different cell types, and used in the research of many different neurological disease models.

View cell types and disease models cited in publications ›

The history behind the B-27 media system

Which B-27 supplement is right for you?

To order the right B-27 supplement for you, choose the application/cell-type tab below and then click on the product name or catalog number to order.

All Gibco™ neural cell culture reagents are optimized to work together as a complete media system. Need a complementary media or cells? Browse our neural cell culture product guide.

Standard Applications / Primary Neurons

Application Product name Cat. No.
Growth and maintenance of neurons B-27 Serum-Free Supplement 17504044

Stem cells and Precursor cells

Application Product name Cat. No.
Proliferation of stem cells B-27 Supplement, minus vitamin A 12587010
Differentiation of stem cells B-27 Supplement, XenoFree CTS™ A14867-01


Specialty applications

Application Product name Cat. No.
Studies where XenoFree components are required B-27 Supplement, XenoFree CTS™ A14867-01
Studies of oxidative stress/damage, apoptosis,
or where free radical damage to neurons occurs
B-27 Supplement,
minus AO (antioxidants)
Studies of insulin secretion or insulin receptors B-27 Supplement,
minus Insulin
Electrophysiology studies B-27 Electrophysiology Kit A1413701
Growth and expansion of retinal ganglion cells B-27 Supplement, Custom 0080085SA

Table 1. Cell types and disease models cited in publications using Gibco neural cell culture reagents

Primary cells Brainstem, cerebellar, cortical, dopaminergic, DRG, enteric, GABAergic, glutamatergic, hippocampal, motor, neural progenitor, pancreatic, parasympathetic, retinal, septal, striatal, substantia nigral, sympathetic ganglia
Stem cells Induced pluripotent stem cell, neural precursor, neural stem cell, neuroepithelial, cardiomyocytes
Glial cells Astrocyte, microglia, oligodendrocytes, radial glia, satellite, schwann
Disease models Alzheimer’s disease, amyotropyic lateral sclerosis (ALS), brain tumor, cerebral palsy, epilepsy, glioma, Huntington’s disease, multiple sclerosis, Parkinson’s disease, schizophrenia, spinal cord injury, stroke