• One reagent convenience for DNA or RNAi transfection
  • Simple protocol for easy optimization in a wide range of cell types
  • Overwhelmingly more publications cite Invitrogen™ Lipofectamine™ 2000 Transfection Reagent

Transfect your cells with confidence

Lipofectamine 2000 reagent delivers DNA or siRNA with excellent transfection performance for protein expression, gene silencing, and functional assays. Superior efficiency and simple protocol for success across a broad range of cell lines (figure 1)—Lipofectamine 2000 reagent does it all.

Figure 1. Lipofectamine 2000 reagent yields high transfection efficiencies in a variety of cell lines. Representative images are shown for the conditions that produced optimal transfection. Blue cells are those that express β-lactamase and therefore have been successfully transfected with the reporter vector. Green cells are untransfected. Invitrogen™ GripTite™ 293 MSR cells untreated (A) and transfected using 0.5 μg of Lipofectamine 2000 per well (B). HeLa cells untreated (C) and transfected using 0.25 μg of Lipofectamine 2000 (D). MCF-7 cells untreated (E) and transfected using 0.5 μg of Lipofectamine 2000 (F). High transfection efficiencies in a variety of cell lines. 

Lipofectamine recognition

Publications citing Lipofectamine 2000 have skyrocketed since its launch in 1999 and continue to climb (Figure 2). Lipofectamine 2000’s huge success can be attributed to its performance, delivering a high level of expression and transfection efficiency on a broad range of cell types. Invitrogen™ Lipofectamine™ reagents are the most cited and recognized reagents available for transfection.

Figure 2. Number of publications citing the use of Lipofectamine 2000 Transfection Reagent since 1999.