SuperScript Reverse Transcriptase
With thousands of references and citations, the SuperScript™ family of reverse transcriptases has proven to be a first choice for cDNA synthesis. SuperScript reverse transcriptases are designed to reliably and efficiently deliver high yields of full-length cDNA. If you need maximum sensitivity for real-time RT-PCR and microarray experiments and cDNA that represents input RNA with maximum fidelity, you need SuperScript reverse transcriptase.
Which SuperScript product is right for you?
|Order now||Order now||Order now||Order now||Order now|
|Goal||Highest performance & convenience RT kit for RT-qPCR||Highest performance & convenience RT kit for RT-qPCR with integrated gDNA removal step||Full optimization with flexible priming RT kit for RT-PCR||Most effective RT for all RNA, including difficult templates||Effective & versatile RT for wide range of RNA templates|
|Product||SuperScript™ IV VILO™ Master Mix
||SuperScript™ IV VILO™ Master Mix with ezDNase||SuperScript™ IV First-Strand Synthesis System||SuperScript™ IV RT
||SuperScript™ III RT|
|Format||cDNA synthesis kit||cDNA synthesis kit with gDNA removal||cDNA synthesis kit||Stand-alone enzyme||Stand-alone enzyme|
|Optimal reaction temperature||50°C||50°C||50–55°C||50–55°C||50°C|
|RT reaction time||10 min||12 min (includes 2 min gDNA removal)||10 min||10 min||50 min|
|Download Protocol||Download protocol||Download protocol||Download protocol||Download protocol||Download protocol|
The reverse transcription step is one of the greatest sources of variation in RT-qPCR. With SuperScript IV reverse transcriptase, the Ct value can be reduced by as much as 8. This enzyme outperforms wild type reverse transcriptases with better sensitivity at lower target concentrations. And it shortens the reaction time to just 10 minutes.
Inhibitors in Reverse Transcriptase Reaction
The reverse transcription step is a major source of variation in RT-qPCR. With SuperScript IV VILO Master Mix, the RT-qPCR workflow is sensitive and fast with highest cDNA yields, which enable more efficient and reproducible data.
SuperScript IV reverse transcriptase is the most robust reverse transcriptase with increased processivity and more resistant to reaction inhibitors such as guanidinum, heparin and salt as compared to wild type reverse transcriptases.
Overcoming RT-qPCR challenges
Sensitivity and dynamic range in reverse transcription are important for accurate quantitation in RT-qPCR. In this video, you will learn the top 5 reasons for low sensitivity and how to overcome these mistakes and challenges.