Since its introduction in 1988, the Invitrogen SuperScript line of reverse transcriptases (RTs) has enabled scientists worldwide to achieve the best performance in cDNA synthesis. Today, with over 50,000 citations, reviews, and publications, SuperScript products are the most highly trusted and widely used RTs.  The latest enzyme introduced to the market, Invitrogen SuperScript IV RT, provides superior performance even with challenging RNA samples. If you seek the best cDNA synthesis efficiency, sensitivity, reproducibility, and reliability with any type of sample RNA, you need SuperScript RTs.

Which SuperScript product is right for you?

  Order Order Order Order Order

  Most effective enzyme for all RNA, including difficult templates Full optimization with flexible priming options for RT-PCR Highest performance and convenience for RT-qPCR Highest performance and convenience for RT-qPCR; with integrated gDNA removal Fast and simple RT-PCR workflow
Product SuperScript IV Reverse Transcriptase SuperScript IV First-Strand Synthesis System SuperScript IV VILO Master Mix SuperScript IV VILO Master Mix with ezDNase  SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity
Format Stand-alone enzyme cDNA synthesis kit cDNA synthesis master mix cDNA synthesis master mix with gDNA removal One-step RT-PCR kit
Optimal reaction temperature 50–55°C 50–55°C 50°C 50°C 55°C
RT reaction time 10 min 10 min
10 min 10 min (additional 2 min gDNA removal) 30 min
  Download protocol Download protocol Download protocol Download protocol Download protocol

Videos

Reverse transcriptase reaction in 10 minutes—enzyme processivity

SuperScript IV RT has very high processivity and requires less time to generate full-length cDNA compared to wild type enzymes. With SuperScript IV RT it is possible to perform cDNA synthesis in only 10 minutes.



Understanding reverse transcriptase—effects on Ct value

SuperScript IV RT outperforms wild type RTs with better efficiency and sensitivity. Therefore, with SuperScript IV RT, the Ct value can be significantly reduced compared to wild type enzymes in RT-qPCR applications.

Inhibitors in reverse transcriptase reaction

Inhibitors can significantly impair the efficiency of reverse transcription reactions. Greatly improved processivity of SuperScript IV RT allows cDNA synthesis even in the presence of common inhibitors such as guanidinium, heparin, and salts.


Overcoming RT-qPCR challenges

Sensitivity and dynamic range in reverse transcription are important factors for accurate quantitation in RT-qPCR. Learn the top 5 reasons for reduced sensitivity and high variability in RT-qPCR applications, and how SuperScript IV VILO Master Mix can help to overcome these challenges.