High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Phusion™ High-Fidelity DNA Polymerase was created by fusing a dsDNA-binding domain to a Pyrococcus-like proofreading polymerase. Due to this unique fusion technique, Phusion DNA Polymerases have extremely low error rates and are considered a gold standard for high-fidelity PCR.

Thermo Scientific high-fidelity DNA polymerase selection table

  Phusion High-Fidelity DNA Polymerase Phusion Hot Start II High-Fidelity DNA Polymerase Phusion Flash High-Fidelity PCR Master Mix Phusion U Hot Start DNA Polymerase
Fidelity vs. Taq 52x 52x 25x 25x
Amplicon size Up to 20 kb Up to 20 kb Up to 20 kb Up to 20 kb
Enhanced specificity
Fast cycling
dUTP incorporation
Stand-alone enzyme
Master mix

*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels

Additional high-fidelity PCR enzymes: