DoubleDigest Calculator—Thermo Scientific
Easily determine optimal reaction conditions for your double digest reaction using this tool. DoubleDigest conveniently calculates the best enzymatic reaction buffer, enzyme concentrations, incubation conditions, and any additives needed in your double digest reaction. Peak DNA digestion without star activity is best accomplished with Thermo Scientific conventional restriction enzymes using the Five Buffer System.
For Research Use Only. Not for use in diagnostic procedures.