Featuring strategies and tools for optimal genome editing efficiencies in mice and zebrafish embryos

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You asked and we listened─introducing a step-by-step protocol designed to guide you through efficient microinjections of CRISPR-Cas9 complexes into mice and zebrafish embryos.

In this protocol you’ll get:

  • A streamlined experimental protocol to genetically engineer the genomes in mice and zebrafish embryos
  • Strategies and tools for efficient embryo microinjection using the CRISPR-Cas9 system
  • Recommendations on optimal conditions including Cas9/guide RNA (gRNA) ratios for high editing efficiencies
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