Learn the basics of Sanger sequencing

DNA Sequencing by Capillary Electrophoresis, Applied Biosystems Chemistry Guide, 3rd edition (2016, 214 pages)

Description: A perfect handbook for scientists and researchers using Sanger sequencing. In this chemistry guide, you will learn the basic principles of Sanger sequencing from workflow to applications.

Topics include:

  • Sanger sequencing applications
  • DNA template preparation
  • Cycle sequencing
  • Purification of extension products
  • Capillary electrophoresis
  • Data analysis
  • Troubleshooting

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 Oncology
 Genetics/inherited disease
 Infectious disease/Pathogen (Virology/Bacteria)
 HLA
 Ecology/Plant/Animal Species identification/Barcode of Life
 Molecular cloning (plasmid confirmation)
 Gene editing, CRISPR, Transgenic model confirmation
 Genotyping (plants, cell line confirmation)
 Human ID/Forensics/Paternity/Cell line Confirmation
 Epigenetics/Methylation
 Next Generation Sequencing Confirmation
 Non-nucleic acid: Proteomics/Glycan analysis
 Others