Making protein sample preparation easy

Biomarker and biotherapeutic characterization and quantitation just got a lot easier. Use the Thermo Scientific™ SMART Digest™ and SMART Digest Immunoaffinity (IA) kits for protein sample preparation that is fast, simple, highly reproducible, and sensitive. 

 

Related protein digestion products
SMART Digest products

All kits provide:

  • Fast, easy, highly reproducible, characterization, and quantitation of proteins
  • In a range of scalable kit options to meet your workflow requirements.
SMART Digest Trypsin Kits Uses trypsin to cleave the C-terminal side of lysine and arginine amino acid residues of peptides.
SMART Digest Chymotrypsin Kits Uses chymotrypsin to cleave the peptide bonds formed by aromatic residues such as tyrosine, phenylalanine, and tryptophan.
SMART Digest Proteinase K Kits Uses proteinase K kits to cleave the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups.
SMART Digest ImmunoAffinity (IA) Streptavidin Kits The Streptavadin version of the kit allows for purification of various biomolecules via the formation of strong streptavidin-biotin bond.
SMART Digest ImmunoAffinity (IA) Protein A Kits The Protein A version of the kit allows for targeted purification of immunoglobulins due to the strong affinity for binding to the Fc portion of the molecule.
SMART Digest ImmunoAffinity (IA) Protein G Kits The Protein G version of the kit allows for targeted purification of immunoglobulins due to the strong affinity for binding to the Fab and Fc portion of the molecule.
Comparison with In-Solution

SMART Digest kit:

  • Is significantly faster
  • Is reproducible protein digestion
  • Provides improved sequence coverage
  • Provides improved data quality
SMART Digest vs. Conventional In-Solution Digest Protocol
SMART Digest benefits
SMART Digest IA versus standard
  • Significantly increased speed of sample processing compared to in-solution digestion
  • 3-step sample preparation protocol
  • Higher throughput
  • Ability to automate
  • More reproducible than in-solution digestion
  • Higher sensitivity

Featured videos
Why SMART Digest kits provide better protein digestion

Fast and reproducible protein digestion for improved sequence coverage and data quality over traditional in-solution digestion. This quick introductory video reviews:

  • The easy protocol
  • Why it’s simpler, quicker, and reproducible—producing quality data
  • Its application in biopharmaceutical and protein research 
Do you want fast, easy and highly reproducible protein digestion?

Learn how SMART Digest kits provide easy, fast, and highly reproducible protein digestion compared to in-solution digestion protocols with this introduction animation.

  • 3-step sample preparation protocol
  • Higher throughput
  • Ability to automate
  • Higher sensitivity

Resources
   Video: BioPharMoore Episode 2: SMARTening up on Protein Digestion