- Ending Repression of the Silent X Chromosome
Anand Minajigi, John E. Froberg, Chunyao Wei, Hongjae Sunwoo, Barry Kesner, David Colognori, Derek Lessing, Bernhard Payer, Myriam Boukhali, Wilhelm Haas, and Jeannie T. Lee
Science. 2015, 17;349(6245)
- Pthalimides and Protein Destruction
Winter, G.E., et al. (2015) "Phthalimide conjugation as a strategy for in vivo target protein degradation," Science 348 (pp.1376-81), doi: 10.1126/science.aab1433
- CRISPR-Cas9 Genomic Editing Characterizes the Prion Protein Knockout Proteome
Mohadeseh Mehrabian, Dylan Brethour, Sarah MacIsaac, Jin Kyu Kim, C. Geeth Gunawardana, Hansen Wang and Gerold Schmitt-Ulms
PLoS One. 2014, 9;9(12):e114594.
- Evaluating multiplexed quantitative phosphopeptide analysis on a hybrid quadrupole mass filter/linear ion trap/Orbitrap mass spectrometer
Brian K Erickson, Mark P Jedrychowski, Graeme C. McAlister, Robert A. Everley, Ryan C Kunz, and Steven P. Gygi
Anal. Chem., 2015, 87 (2), 1241–1249
- A draft map of the mouse pluripotent stem cell spatial proteome
Andy Christoforou, Claire M. Mulvey, Lisa M. Breckels, Aikaterini Geladaki, Tracey Hurrell, Penelope C. Hayward, Thomas Naake, Laurent Gatto, Rosa Viner, Alfonso Martinez Arias & Kathryn S. Lilley
Nat Commun. 2016 Jan 12;7:9992. doi: 10.1038/ncomms9992
- The paracaspase MALT1 cleaves HOIL1 reducing linear ubiquitination by LUBAC to dampen lymphocyte NF-kB signalling
Theo Klein, Shan-Yu Fung, Florian Renner, Michael A. Blank, Antoine Dufour, Sohyeong Kang, Madison Bolger-Munro, Joshua M. Scur, John J. Priatel, Patrick Schweigler, Samu Melkko, Michael R. Gold, Rosa I. Viner, Catherine H. Re'gnier, Stuart E. Turvey and Christopher M. Overall
Nature Communications 6, Article number: 8777 doi:10.1038/ncomms9777
- Lenalidomide induces ubiquitination and degradation of CK1a in del(5q) MDS
Jan Kronke, Emma C. Fink, Paul W. Hollenbach, Kyle J. MacBeth, Slater N. Hurst, Namrata D. Udeshi, Philip P. Chamberlain, D. R. Mani, Hon Wah Man, Anita K. Gandhi, Tanya Svinkina, Rebekka K. Schneider, Marie McConkey, Marcus Jaras, Elizabeth Griffiths, Meir Wetzler, Lars Bullinger, Brian E. Cathers, Steven A. Carr, Rajesh Chopra & Benjamin L. Ebert
Nature. 2015, 523(7559), 183-188
- Quantitative mass spectrometry-based multiplexing compares the abundance of 5000 S. cerevisiae proteins across 10 carbon sources
Joao A. Paulo, Jeremy D. O'Connell, Robert A. Everley, Jonathon O'Brien, Micah A. Gygi, and Steven P. Gygi
Journal of Proteomics 148 (pp 85 -93), doi: 10.1016/j.jprot.2016.07.005
SPS MS3 Workflows
Maximize quantitative accuracy and precision
The synchronous precursor selection (SPS)-based MS3 technology on the Orbitrap Fusion Tribrid systems provide a unique, unmatched capability to accurately measure the most subtle changes in low-abundance proteins. This workflow allows for simultaneous quantitative analysis of ten samples, with significantly improved accuracy achieved by reducing the co-isolation of tagged interferences. The increased sensitivity and ion transmission boosts the number of quantifiable peptides present at low levels, enabling scientists to perform comprehensive proteome-wide quantitation faster and more accurately than ever before.
Featured TMT multiplexing technologies
Scientists pushing the limits of quantitation and protein characterization require more expansive and in-depth analytical information. The Orbitrap Fusion Lumos is the newest Tribrid mass spectrometer providing the highest sensitivity, highest selectivity and lowest detection limit. It was uniquely developed to expand the Tribrid performance in advanced -omics and biopharma workflows, including low level quantitation, data independent and top down analyses.
Isobaric chemical tags represent a fast, unbiased, and sensitive method to quantify almost all proteins in a biological sample. The TMT10plex Label Reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex Reagents but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter.
What is Synchronous Precursor Selection?
The SPS method begins with selection of the parent ion in the MS scan, followed by its isolation in the quadrupole and fragmentation by collisionally induced dissociation (CID) in the ion trap. Following fragmentation, SPS enables simultaneous isolation of up to 20 MS2 fragment ions. A select group of MS2 fragment ions are then transferred back into the ion routing multipole (IRM) where they undergo higher energy collisional dissociation (HCD) fragmentation, with the MS3 fragments then detected in the Orbitrap analyzer.
The use of SPS dramatically increases the reporter ion signal intensity and improves the ratio accuracy, due to improved counting statistics, leading to a significant increase in the number of quantified peptides.
SPS MS3 webinars
SPS MS3 videos
SPS MS3 citations
Groundbreaking biological research enabled by technological advances in quantitative proteomics
There has been a recent and significant increase in groundbreaking biological research, relying on the TMT SPS MS3 method. The citations below are a small representation, indicating how cell biologists have capitalized on the use of this new method to help answer complex biological questions. The average impact factor for all published articles is approximately 15, about twice the impact factor of publishing in other reputable journals.