Tom Osborn, Director of Molecular Breeding Technology, Monsanto
Genotyping at Monsanto has been transformed through a series of technology innovations to the current state of automated workflows that deliver high-volume genetic data for key decisions in product advancement. Some recent innovations utilizing genome-wide selection and data from genotyping by sequencing have led to our ability to conduct yield testing in the lab. The success of this new workflow is dependent on collaborations with internal and external technology partners to deliver scalable, low-cost, and rapid lab processes.
Tom Scott, CEO, Informa Agribusiness Intelligence IEG
The world’s agriculture market is dynamic and subject to constant change. Investments in agricultural technologies and innovations are driven by the underlying economics of commodities and the markets they support. This session will provide both short- and long-term perspectives on the impact of mega-trends, trade, industry consolidation, and other market drivers on innovation and technology in agriculture.
Wim van Haeringen, PhD, CEO, VHLGenetics
As a pioneer with a successful history of applying molecular technologies to animal genotyping, VHLGenetics is again leading the way with the application of genotyping by sequencing (GBS). VHL will present the output of a collaborative effort with Thermo Fisher Scientific on feline, canine, and bovine GBS panels for detecting genetic variants and conducting parentage testing.
Dr. Dario Grattapaglia, Senior Research Scientist and Project Leader, Brazilian Agricultural Research Corporation (Embrapa)
The identification of new sources of genetic variation is a key component of any long-term breeding strategy to increase the productivity, resilience, and sustainability of crop varieties. Over six million crop accessions are currently being conserved worldwide in still largely untapped gene banks. High-throughput, low-cost, and robust SNP genotyping technologies are necessary to unlock this wealth of diversity and release the data in an organized and user-friendly fashion to allow adoption by germplasm curators and breeders.
Emily Rose, Sequencing Lab Supervisor, GeneSeek-Neogen
Bacterial classifications and phylogenetic studies have been largely improved by the targeted amplicon sequencing of the hypervariable regions within the 16S rRNA gene, which is represented in all bacteria. However, limiting the classification investigation to 16S sequencing can still lead to difficulty in discerning closely related organisms down to the species level. To aid in the pursuit of accurately identifying bacterial species and serotypes (strains), Thermo Fisher Scientific has recently created the Applied Biosystems Axiom Microbiome Array as an effective assay to distinguish thousands of microorganisms at the species and even serotype level.
We have assembled a panel of primers for coho salmon that produces 360 amplicons containing approximately 440 single nucleotide polymorphisms (SNPs), with all sequences amplified via a single polymerase chain reaction. Application of the panel to coho salmon from hatchery populations in southern British Columbia (BC) has indicated substantial differentiation among populations, as well as differentiation among brood years and individuals within hatcheries.
Sugar beet is one of the world’s most important crops, currently supplying around 20% of the sugar consumed worldwide. The development of varieties that require less technical inputs for cultivation is one of the main research goals in sugar beet. To achieve this, sugar beet breeding is focusing on genetic improvement programs assisted by SNP (single nucleotide polymorphisms) molecular markers.
A number of global dynamics are changing the agriculture and food ecosystem. The simplicity, accessibility, and ease of use of molecular technologies and next-generation sequencing are reshaping the animal and plant breeding sciences. Thermo Fisher Scientific products and services offer high quality, innovative life science solutions for every plant and animal research or breeding lab.
Through the use of Ion AmpliSeq™ custom panels, GeneSeek—a Neogen Corporation subsidiary—has designed a primer pool that is able to target ~4,000 SNP loci which allows certain traits such as tenderness and parentage to be identified in cattle. Within this process, GeneSeek has modified the Ion AmpliSeq protocol in order to sequence at least 192 samples in one run.
Genotyping by sequencing (GBS) is a cost-effective alternative for obtaining a large number of markers across a large sample size, but markers are randomly distributed throughout the genome. Alternately, enrichment coupled with next-generation sequencing is a cost-effective strategy for obtaining sequence information for loci of interest across a large sample.