Case Study: Improving plant breeding using Ion AmpliSeq™ and Ion Torrent™ sequencing technologies for targeted resequencing and SNP analysis in tomato
High-throughput characterization of known and novel SNPs is critical for plant genotyping applications. Recent advances in NGS have simplified plant SNP discovery and validation and have also created a demand for high-throughput SNP biomarker screening for plant breeding program improvements. In this study we used the Ion AmpliSeq™ panel workflow to genotype 370 SNPs from 192 matched paired tomato leaf samples (purified DNA and crude leaf lysate) on the Ion PGM™ Sequencer. The results showed a call rate of 94% and a call concordance of 99% between purified DNA and leaf lysates.