ELISA and Multiplex Immunoassays

Choose high-quality Invitrogen immunoassays for protein quantitation

Rely on our 25 years of experience manufacturing and supplying antibody-based, target-specific protein quantitation reagents, kits, and panels for life science research. These products, which are collectively called immunoassays, include matched antibody pairs, uncoated and coated-plate ELISA kits, and multiplexing assays for the Luminex™ platform. All are validated for reliability and specificity. Select from hundreds of ready-to-ship kits or configure and order a custom assay panel.

To find the best assay for your needs, choose from popular immunoassay categories below or contact our field application specialists (FAS), who can answer your questions and provide you with individualized immunoassay support, including information about new immunoassay technologies.

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Multiplexable:No Sample volume used:50 μL Best used for:Validation Catalog sizes:1 plate (96 tests), 2 plates, 5 plates Dynamic range:2–3 logs Sensitivity:pg/mL Incubation time:~4 hr
Build-it-yourself immunoassays
Assays for the Luminex™ platform enable simultaneous measurement of multiple proteins per well using as little as 25 μL of sample. This technology produces results comparable to ELISA but with greater efficiency, speed, and dynamic range. Mix and match your own custom panel using singleplex kits, choose ready-to-use multiplex panels, or have us create a custom panel for you.
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Luminex immunoassay features to make the best choice for your current project
Coated ELISA kits
Multiplexable:Yes Sample volume used:25 μL Best used for:Screening Catalog sizes:1 plate (96 tests) Dynamic range:3–4 logs Sensitivity:pg/mL Incubation time:~3.5 hr
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Multiplexable:No Sample volume used:50 μL Best used for:Validation Catalog sizes:N/A Dynamic range:N/A Sensitivity:N/A Incubation time:N/A
Which immunoassay platform are you looking for?
Multiplexable:No Sample volume used:50 μL Best used for:Validation Catalog sizes:2 plates, 10 plates, 20 plates Dynamic range:2–3 logs Sensitivity:pg/mL Incubation time:~24 hr
Uncoated ELISA kits
Invitrogen Assays for the Luminex Platform
Luminex immunoassays
Coated ELISA Kit features to make the best choice for your current project
This extensive portfolio includes over 800 ready-to-use ELISA kits and is optimized for convenient and sensitive quantitation of a wide range of targets in various sample types, including human, mouse, rat IgG, cytokines, phosphorylated proteins, beta amyloids, extracellular and intracellular targets. These ELISA kits include pre-coated plates with capture/detection antibodies, standards, and buffers and accessory reagents
Coated ELISA kits 
Include validated matched pairs of antibodies and calibrated standard for custom ELISA development. Using the recommended buffers described in the detailed protocol, these kits provide bulk antibody pairs for analyzing five to forty 96-well plates. The reagents are optimized for reliable performance using the specific antibody concentrations described in the protocol, but researchers can modify and re-optimize the protocol as needed.
Uncoated ELISA kits 
Uncoated ELISA Kit features to make the best choice for your current project
A variety of buffers, reagents and accessories in addition to validated antibody pairs or over 50k singular validated antibodies to choose from all to give you the flexibility to create your own immunoassay for protein detection and quantification.
Build-it-yourself immunoassays

Quiz: test your immunoassay IQ