A commitment to antibody performance

Antibodies are some of the most critical research reagents used in the lab. Poor specificity or application performance can significantly frustrate the ability to obtain good results, which can cause critical delays. Underperforming antibodies result in a lack of reproducibility, wasting time and money. In other words, researchers need antibodies that bind to the right target and work in their applications every time. To help ensure superior antibody results, we've expanded our specificity testing methodology using a 2-part approach for advanced verification.

Invitrogen antibodies are currently undergoing a rigorous 2-part testing approach

Part 1—Target specificity verification

This helps ensure the antibody will bind to the correct target. Our antibodies are being tested using at least 1 of the following methods to ensure proper functionality in researcher’s experiments. Click on each testing method in the table below for detailed testing strategies, workflow examples and data figure legends.

Immunoprecipitation-mass spectrometry
(IP-MS)
Genetic modification Independent antibody verification (IAV) Biological verification

IP-mass spectrometry—testing using immunoprecipitation followed by mass spectrometry to identify antibody targets

Knockout—expression testing using CRISPR-Cas9 cell models
Knockdown—expression testing using RNAi to knockdown gene of interest

IAV—measurement of target expression is performed using two differentially raised antibodies recognizing the same protein target

Cell treatment—detecting downstream events following cell treatment
Relative expression—using naturally occurring variable expression to confirm specificity
Neutralization—functional blocking of protein activity by antibody binding
Peptide array—using arrays to test reactivity against known protein modifications
Orthogonal method—correlation using both antibody dependent and independent method

Part 2—Functional application validation

These tests help ensure the antibody works in a particular application(s) of interest, which may include
(but are not limited to):

  • Western blotting
  • Flow cytometry
  • ChIP
  • Immunofluorescence imaging
  • Immunohistochemistry

Most antibodies were developed with specific applications in mind. Testing that an antibody generates acceptable results in a specific application is the second part of confirming antibody performance.

Learn more about our 2-part validation testing approach in this easy-to-read, one-page document. It summarizes our comprehensive verification testing process and describes how we provide a new standard of confidence for our customers.

 Download the flyer

Featured videos

Antibody validation through advanced specificity verification

Addressing the antibody reproducibility crisis: A panel discussion with key scientific leaders

Validation standards for reproducibility

Thermo Fisher Scientific is committed to adopting validation standards for our Invitrogen antibody portfolio that are tested for both target specificity and functional application. By supporting the International Working Group for Antibody Validation (IWGAV), and adopting their recommendations, we’re doing our part to ensure reproducibility and proper functionality in the scientific community.

Resources on antibody verification

Learn more about efforts from the research community to develop and implement standards for antibody validation.

IWGAV paper

Workshop report and session recordings

*The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic uses.