Cyclophilin D (CyPD) is a member of the cyclophilin family of peptidylprolyl isomerases. Cyclophilin D is located in the matrix of the mitochondria where it acts as an integral member of the permeability transition pore complex, which also includes the voltage dependent anion channel (VDAC) and the adenine nucleotide translocator (ANT). Activation of this pore is involved in the induction of apoptotic and necrotic cell death. Cyclophilin D is conserved, ubiquitous, and abundantly expressed in most cells, making it a good loading control.

We offer a number of quality Invitrogen cyclophilin D antibodies for a variety of research needs.

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Western blot analysis was performed on whole cell extracts. The blots were probed with Anti-Cyclophilin D Rabbit Polyclonal Antibody (Cat. No. PA3-023) and detected by chemiluminescence using Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Cat. No. G-21234). A 40 kDa band corresponding to cyclophilin D was observed across the cell lines tested; additional bands at 21 and 35 kDa may correspond to other cyclophilin isoforms. 


Immunohistochemical analysis was performed on deparaffinized human hepatocarcinoma biopsy tissues. Tissues were probed with a rabbit polyclonal antibody recognizing cyclophilin D (Cat. No. PA3-022) or treated equivalently without primary antibody (negative control). Tissues were counterstained with hematoxylin and prepared for mounting.


Immunofluorescence analysis of cyclophilin D in A431 cells. Cells were probed with a cyclophilin D polyclonal antibody (Cat. No. PA3-022) at a dilution of 1:200, or treated equivalently without primary antibody (negative control) and incubated with a secondary antibody conjugated to DyLight 488 dye (Cat. No. 35503). The resultant staining of cyclophilin D (green), F-actin with phalloidin (red), and nuclei with DAPI (blue) is shown.


Annotated product references

PA1-028 was used in western blotting to expand our understanding of the mechanisms involved in Pb-induced mitochondrial apoptosis. Liu G, Wang ZK, Wang ZY et al. (2016) Mitochondrial permeability transition and its regulatory components are implicated in apoptosis of primary cultures of rat proximal tubular cells exposed to lead. Arch Toxicol 90(5): 1193–1209.

PA3-022 was used in western blotting to explore the effects of FK506-binding protein 51 (FKBP51) deletion on adipogenesis. Stechschulte LA, Hinds TD, Khuder SS et al. (2014) FKBP51 controls cellular adipogenesis through p38 kinase-mediated phosphorylation of GRa and PPARg. Mol Endocrinol 28: 1265–1275.

PA3-022 was used in western blotting to identify cyclophilin-40–interacting proteins and the cellular functions of cyclophilin-40. Park MS, Chu F, Xie J et al. (2011) Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40. Anal Biochem 410: 257–265.

PA1-028 was used in western blotting to investigate the effect of CypD on mitochondrial permeability regulation in liver and brain. Hansson MJ, Morota S, Chen L, et al. (2011) Cyclophilin D-sensitive mitochondrial permeability transition in adult human brain and liver mitochondria. J Neurotrauma 28: 143–153.

PA1-028 was used in western blotting to investigate the association between spatially distinct mitochondrial proteomes and dysfunction in the type 2 diabetic heart. Dabkowski ER, Baseler WA, Williamson CL et al. (2010) Mitochondrial dysfunction in the type 2 diabetic heart is associated with alterations in spatially distinct mitochondrial proteomes. Am J Physiol Heart Circ Physiol 299: H529–540.


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