Validated for a variety of applications
- Western analysis
- Flow cytometry
Anti-GFP conjugated to Alexa Fluor® 488 with target BacMam-GFP
Alexa Fluor® 488 dye was conjugated to two independent lots of GFP Rabbit IgG Polyclonal antibody resulting in anti-GFP Alexa Fluor® 488 (catalog number A21311). U2OS cells were transduced with CellLight® Mitochondria-GFP, BacMam 2.0 (catalog number C10508). After an overnight incubation the cells with treated with fixation and then permeablized. GFP molecules were detected by the addition of anti-GFP Alexa Fluor® 488 (catalog number A21311). Texas Red®-X phalloidin (catalog number T7471) was added to detect actin. Hoeschst 33342 (catalog number H3570) was used as a nuclear counterstain.
Recovering GFP signal after Click-iT ® EdU treatment
Human osteosarcoma cells transduced with CellLight® Mitochondria-GFP (catalog number C10508) samples were pulsed with EdU, fixed, and labeled with Click-iT® EdU Alexa Fluor® 555 (catalog number C10338) for cell proliferation (pink). DAPI for nuclei (blue) (catalog number D3571). GFP signal was recovered with anti-GFP Alexa Fluor® 488 (catalog number A21311) primary antibody (green).
Order anti-GFP antibodies
|A6455||GFP Rabbit Serum Polyclonal Antibody||100 µL|
|A10262||GFP Chicken IgY Antibody Fraction||100 µL|
|A11122||GFP Rabbit IgG Polyclonal Antibody Fraction||100 µL|
|A11120||GFP (clone 3E6) Mouse IgG2a, Monoclonal Antibody||100 µg|
|A11121||GFP (clone 11E5) Mouse IgG1, Monoclonal Antibody||100 µg|
|G10362||GFP Monoclonal Antibody, ABfinity™ Recombinant||100 µg|
|R10367||Red Fluorescent Protein (RFP) Rabbit Polyclonal Antibody, Purified||100 µg|
|A21311||GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 488 Conjugate||100 µL|
|A31851||GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 555 Conjugate||100 µL|
|A21312||GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 594 Conjugate||100 µL|
|A31852||GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 647 Conjugate||100 µL|
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For Research Use Only. Not for use in diagnostic procedures.