Secondary antibodies are used for the indirect detection of target antigens. They provide increased sensitivity through signal amplification, and greater flexibility for labeling and detection. We offer an extensive selection of high-quality conjugated and unconjugated secondary antibodies that can be used for the fluorescent, colorimetric, and chemiluminescent detection of primary antibodies in a wide range of applications, such as cell imaging, flow cytometry and western blotting.
How to find a secondary antibody
Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.
Secondary antibody selection table
|Reactivity||Non fluorescent conjugates||Fluorescent
|NEW Superclonal™ Secondary Antibodies - A breakthrough in recombinant antibody technology designed to eliminate cross-reactivity in detection of primary antibodies|
Western blot reagents and instruments, chemiluminescent, colorimetric, and fluorescent detection substrates, blocking buffers, and secondary antibody conjugates
We offer a wide selection of ELISA kits targeted to disease-related proteins for research in immunology, cancer, neurodegeneration, stem cells, and more.
Everyone starting out in fluorescence imaging has questions. Learn the fundamentals of fluorescence microscopy, including sample and media considerations, sample preparation, and beginner’s protocols to help you get started. We hope you will benefit from the information in Molecular Probes® School of Fluorescence.
For Research Use Only. Not for use in diagnostic procedures.