POROS® A, G, and CaptureSelect™ HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of Protein A, Protein G, and CaptureSelect™ ligands (Figure 1). The combination allows 3-minute quantitation of immunoglobulins, fusion proteins, Fabs, and biospecific antibodies.
POROS Protein A, Protein G and CaptureSelect Columns
New POROS® Ligands Provide Alternative Specificities
High Speed with High Specificity
The combination of Protein A, Protein G, and CaptureSelect™ affinity ligands when combined with POROS® 20 µm resin provides a high flow rate, high-performance chromatography solution for the rapid quantitation and small-scale purification of:
An example of the chromatographic performance of POROS® CaptureSelect™ LC-Kappa with five unique kappa Fab fragments is shown in Figure 2a. The associated standard curves, Figure 2b, exhibit excellent linearity.
POROS® Affinity HPLC Columns
POROS® A, G, and ligands are available in convenient prepacked HPLC columns. The column range addresses analytical and preparative requirements for the quantitation and purification of immunoglobulins or Fc fusion or HSA fusion proteins.
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