AAV

The unique CaptureSelect ligand technology is combined with the large pore structure of POROS resins to enable the development of large biomolecules such as viral vectors and vaccines. These resins offer high selectivity and high capacity that result in robust, improved yields and purity from crude material in a rapid, one-step purification process.

POROS CaptureSelect AAV8 and AAV9 are the first affinity chromatography resins for the purification of viral vectors that are in use for gene therapy applications, specifically intended for the purification of adeno-associated virus subtypes 8 (AAV8) and 9 (AAV9).

The POROS CaptureSelect AAV8 and AAV9 affinity resins are designed to significantly improve downstream processing by reducing the number of purification steps and offering scalability, thus maximizing processing consistency and productivity.

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POROS CaptureSelect resins and reagents for viral vector purification

The large pore structure of POROS CaptureSelect AAV8 and AAV9 affinity resins makes them suited for purifying large, complex AAV vectors, supporting chromatographic separations at considerably faster flow rates than conventional liquid chromatography separations while maintaining high dynamic binding capacity. In addition, the 50 μm particle size provides superior resolution for excellent single-step impurity clearance that is independent of scale and flow rate. These affinity resins address the high-selectivity and high-capacity requirements for purifying viral vectors, exhibiting capacities of >1014 viral genomes per milliliter (vg)/mL (Table 1).

 

Ligand selectivity

Binding capacity (vg/mL)

pH stability

POROS CaptureSelect AAV9

AAV9

>1014

pH range 1–10

POROS CaptureSelect AAV8

AAV8

>1013

pH range 1–10

Table 1. Summary of POROS CaptureSelect AAV8 and AAV9 affinity resin properties. 


POROS CaptureSelect resins and reagents for purification of viral vectors

1. Terova O, Parra S, Clasen R et al. (2016) Innovative Downstream Purification Solutions for Viral Vectors: Enabling Platform Approaches to Advance Gene Therapies. BioProcess†International.


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Resources

Bioproduction resources- Have questions about Gibco media, POROS chromatography resins, Applied Biosystems SEQ rapid contaminant tests, or cell therapy products? Click below to access product brochures, training videos, mobile apps, and more.

For research use or further manufacturing, not for diagnostic use or direct administration in humans or animals.