The Thermo Scientific HyPerforma Single-Use Fermentor (S.U.F.) is pioneering the single-use technology market by delivering a unique and rigorous solution for microbial fermentation applications while offering the flexibility, ease of use, and efficiency found in single-use systems.
The S.U.F. is engineered for optimal oxygen mass transfer, mixing, and temperature control and performs comparably to conventional stainless steel systems. S.U.F.s use fundamental engineering principles, helping to make scale-up and process development work readily streamlined. Available in 30 L and 300 L sizes, the S.U.F. consists of a fermentor tank and a standard Thermo Scientific HyPerforma S.U.F. BioProcess Container (BPC).
Benefits of single-use fermentors
Purpose built for results
The S.U.F. delivers an optimal growth environment through powerful agitation with three Rushton impellers and baffles, high gas flow rates, and efficient cooling through a greater vessel surface area. The S.U.F. is designed to help you deliver results for your fermentation processes.
Stainless steel performance with single-use benefits
The S.U.F. has been developed with standard stainless steel fermentation design geometry (3:1 height to diameter, 5:1 turndown ratio), making scale-up and process development activities easy. The single-use format helps reduce batch turnover activities, including the time-consuming clean-in-place (CIP) and sterilization-in-place (SIP) cycles, helping to decrease costs and increase productivity.
Constructed using our industry-leading films (Thermo Scientific CX5-14 and Aegis 5-14 films), our robust S.U.F. BioProcess Containers (BPCs) maintain sterility throughout the fermentation process. The containers are highly configurable, allowing you flexibility to address specific application needs.
Our open architecture approach allows you to integrate the S.U.F. with the control unit of your choice. Additionally, we offer turnkey systems, shipped as an S.U.F. and integrated controller.
Available in turnkey format
- Complete mixing system with a water jacket for temperature control
- Drive shaft inserts into the BPC through the mixing drive motor and locks into the BPC agitator assembly of the S.U.F. BPC
Supplied sterile and ready to use
- Agitator assembly is a single-use (polyethylene) impeller with a bearing/seal assembly linked to an external mixer drive
- Gas control with drilled-hole spargers
- Exhaust management system with options for multiple vent filters based on gas flow needs
- Integrally sealed ports in the S.U.F. BPC allow for addition of sensor probes and line sets
- Options include CX5-14 and Aegis 5-14 films
System options (adaptable to your needs)
- Exhaust condenser unit
- Exhaust gas vent filter heater
- Integrated foam sensor
- Load cells (3)
- Cable management tree
- Process control system
- Optional electrical box for remote agitation control
S.U.F.s require a separate external temperature control unit. You may choose an open-architecture approach or a turnkey, ready-to-use S.U.F. system.
High oxygen transfer with minimal liquid loss
Controlled, flexible delivery of gas is supplied through our unique drilled-hole sparge design. Coupled with powerful agitation for gas dispersion, the S.U.F. enables you to optimize oxygen transfer (kLa) to meet your process needs. The innovative exhaust management and condenser systems are designed to provide reliable off-gassing and help minimize liquid loss during the fermentation process.
Powerful mixing for high mass transfer
The S.U.F. delivers powerful agitation through 3 Rushton impellers and a baffled vessel. This mixing facilitates high oxygen transfer and a homogeneous reaction.
Increased cooling capacity
The S.U.F.’s proven 3:1 height-to-width aspect ratio optimizes the surface area for cooling. With this greater surface area and column height, the S.U.F. has better cooling capacity compared to less optimal 2:1 aspect ratio of other culture vessel designs.
Automated foam control
Depending on the fermentation and conditions, foaming may occur during the mixing process. We’ve developed a proprietary single-use foam-sensing probe that allows you to automate foam control and help reduce the risk of contamination or exhaust filter failure.