Cell Analysis Antibodies Information

Learn about a wide range of applications and techniques related to using primary and secondary antibodies in your cell biology research. Topics include immunocytochemistry, immunohistochemistry, immunofluorescence, immunophenotyping using flow cytometry, and western blot analysis.

Cell analysis antibodies features

BioProbes article

BioProbes article

Optimize immunodetection with recombinant secondary antibodies

Sensitive immunodetection in cells and tissues requires high-affinity antibodies that exhibit minimal background staining. Invitrogen Superclonal secondary antibodies represent a breakthrough in recombinant antibody technology, providing sensitive binding to their targets and very low levels of nonspecific staining.

BioProbes article

BioProbes article

Monoclonal antibodies for studying human pluripotent stem cells

Both differentiated and undifferentiated stem cells are identified and characterized using primary antibodies to key targets, which are detected by fluorescence-based methods such as immunofluorescence imaging and flow cytometry. This article features the characterization of new antibodies for stem cell research.


Cell analysis antibodies subtopics


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Type Title Categories
Application note (2011) Six-color mouse lymphocyte immunophenotyping using the Attune Acoustic Focusing Cytometer with red laser option  Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles
Application note (2011) Immunophenotyping peripheral blood cells: A no-lyse, no-wash, no cell loss method for immunophenotyping nucleated peripheral blood cells using the Attune Acoustic Focusing Cytometer  antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, particles
Application note (2011) Mouse immunophenotyping: A no-lyse, no-wash, and no cell loss method using the Attune Acoustic Focusing Cytometer  antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, particles
Application note (2011) Detection of phosphoproteins using the Attune Acoustic Focusing Cytometer  Alexa Fluor, antibodies, Attune/Attune NxT, cell signaling, flow cytometer/flow cytometry, fluorescent dyes, protein detection
Application note (2011) Detection of human mesenchymal stem cells using the Attune Acoustic Focusing Cytometer  Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, stem cell research
Application note (2012) Detecting human circulating endothelial cells using the Attune Acoustic Focusing Cytometer  Alexa Fluor, antibodies, Attune/Attune NxT, endothelial cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection
Application note (2013) Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell colonies  Alexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research
Application note (2013) Multiplexed mitosis and apoptosis analysis  antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles
Application note (2015) Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
Application note (2015) Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
Application note (2015) Detection of murine regulatory T cells on the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
Application note (2015) Attune NxT Flow Cytometer for 6-Color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
Application note (2015) Quantitation of proliferating cells with the EVOS FL Auto Imaging System antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis
Application note (2015) No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immune system, immunophenotyping, particles, pH detection
Application note (2015) 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells Alexa Fluor, antibodies, Attune/Attune NxT, cancer, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Application note (2016) Blood cell counting using the Countess II FL Automated Cell Counter automated cell counter, brightfield microscopy, cell counting, cell counting sample preparation, Countess, EVOS, fluorescent dyes, immunophenotyping, ReadyProbes
Application note (2017) Detection of platelets in whole blood using the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, immunophenotyping, platelets
Application note (2017) Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes antibodies, Attune/Attune NxT, cardiomyocytes, cell differentiation, flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunophenotyping, stem cell research
BioProbes article (2006) Multicolor immunodetection using the violet laser  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
BioProbes article (2006) A guide to antibody labeling and detection  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), multicolor flow cytometry, nanocrystals, Qdot, Zenon
BioProbes article (2006) Develop your own fluorescence-based ELISA  ELISA, antibody labeling,fluorescent dyes, protein quantitation
BioProbes article (2007) Measuring proteins in JAK-STAT signaling  ELISA, antibodies, colorimetric, fluorescent dyes, Luminex, microplate reader, multiplex ELISA/Luminex, particles, protein quantitation
BioProbes article (2007) Mercator multiplex phosphoantibody-based array  antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, protein labeling, protein quantitation
BioProbes article (2007) Looking for apoptosis?  antibodies, apoptosis, colorimetric, ELISA, flow cytometer/flow cytometry,fluorescent dyes, immunocytochemistry (ICC), protein detection, western blotting
BioProbes article (2007) Effortless protein conjugations for small animal in vivo imaging  Alexa Fluor, antibody labeling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging
BioProbes article (2007) The latest in stem cell research  antibodies, antibody labeling, cell differentiation, cell expansion, flow cytometer/flow cytometry, fluorescent dyes, growth factors, fluorescence microscopy/fluorescence imaging, stem cell research
BioProbes article (2007) Simultaneously visualize surface and intracellular markers  antibody labeling, cell fixatives, flow cytometer/flow cytometry, fluorescent dyes, sample preparation, Zenon
BioProbes article (2007) Mitochondria and cardiac disorders  antibodies, antibody labeling, cardiovascular system, cell structure-mitochondria, fluorescent dyes, fluorescence microscopy/fluorescence imaging, oxidative stress, western blotting
BioProbes article (2007) Optimally labeled fluorescent antibodies for in vivo animal imaging  Alexa Fluor, antibody labeling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging
BioProbes article (2007) Multiparametric approaches for studying apoptosis  Alexa Fluor, antibodies, apoptosis, cell structure-mitochondria, cell structure-plasma membrane, enzyme activity detection, flow cytometer/flow cytometry, fluorescent dyes, viability
BioProbes article (2008) Single-step isolation of functional human NK cells  antibodies, cell isolation, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, sample preparation
BioProbes article (2008) Nuclear receptor antibodies: A core technology  antibodies, cell signaling, ELISA, gene expression, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), ion flux/ion channels, western blotting
BioProbes article (2008) Fluorescent protein–based Organelle Lights and new Cellular Lights BacMam reagents  BacMam technology, cell structure-all, cell tracking, Cellular Lights, fluorescent proteins, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC)
BioProbes article (2008) IgG antibody labeling from A to Z—Alexa Fluor to Zenon IgG Antibody Labeling Kits for imaging and flow cytometry  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), protein labeling, Zenon
BioProbes article (2008) Analyzing signaling events with phosphorylation site–specific antibodies—Insights into functional proteomics  antibodies, antibody labeling, cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), protein labeling
BioProbes article (2009) Accurate compensation is as easy as AbC  calibration, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles
BioProbes article (2009) Novel recombinant monoclonal antibodies—ABfinity technology ensures performance  antibodies, cell signaling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), protein quantitation, sample preparation, western blotting
BioProbes article (2009) Baculoviral transduction of human neurons  chemiluminescence, ELISA,fluorescent dyes, gene expression, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), neuroscience, stem cell research, western blotting
BioProbes article (2009) Fluorescence-based alternatives to chromogenic immunohistochemistry  antifades, cancer, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), particles, protein quantitation, sample stability
BioProbes article (2009) Highly specific antibodies for cell junction studies  Alexa Fluor, antibodies, cell junction studies, ELISA, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), particles, western blotting
BioProbes article (2009) Measuring insulin receptors in cell lysates  antibodies, cell signaling, colorimetric, ELISA,Insulin studies, particles, protein quantitation, proteins, western blotting
BioProbes article (2009) Resources for cytokine research  antibodies, cell signaling, chemiluminescence, ELISA, flow cytometer/flow cytometry,Luminex, multiplex ELISA/Luminex, particles, protein detection, protein quantitation, proteins,
BioProbes article (2009) A fluorogenic, target-specific reagent for small animal in vivo imaging  Alexa Fluor, antibody labeling, cancer, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging, protein labeling, sample preparation
BioProbes article (2009) Tools for the study of Alzheimer's disease  antibodies, ELISA, flow cytometer/flow cytometry, Luminex, multiplex ELISA/Luminex, neuroscience, particles, protein detection, protein quantitation, proteins
BioProbes article (2009) Mitochondrial fission and fusion in neurodegeneration  antibodies, BacMam technology, cell structure-mitochondria, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, fluorescence microscopy/fluorescence imaging, microplate reader, neuroscience
BioProbes article (2009) A growing list of tools for the violet laser  Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability
BioProbes article (2009) Interrogating potential drug targets for Parkinson's disease  antibodies, cell signaling, enzyme activity detection, fluorescence microscopy/fluorescence imaging, neuroscience, proteins, substrates
BioProbes article (2009) Specific detection of gap junction proteins  Alexa Fluor, antibodies, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), protein detection
BioProbes article (2009) Is it time to go direct? IgG antibody labeling methods as versatile as your imagination  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, immunocytochemistry (ICC), immunohistochemistry (IHC), nanocrystals, Qdot, sample preparation, Zenon
BioProbes article (2009) Novel solid-phase antibody labeling  Alexa Fluor, antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, sample preparation, Zenon
BioProbes article (2009) Characterization of type II kinase inhibitors and nonactivated kinases  antibodies, enzyme activity detection, fluorescent dyes, fluorometer
BioProbes article (2010) Fast and efficient solid-phase antibody labeling antibodies, antibody labeling 
BioProbes article (2010) Highly selective antibodies for GFP and RFP antibodies, fluorescence microscopy/fluorescence imaging, fluorescent proteins
BioProbes article (2010) Fluorescent dye labeling made simple antibody labeling, fluorescent dyes, labeling, protein labeling
BioProbes article (2010) Optimal pairing of dyes and antibodies for flow cytometry antibodies, flow cytometer/flow cytometry,multicolor flow cytometry, particles, Qdot
BioProbes article (2011) A no-lyse, no-wash, no–cell loss method for immunophenotyping—Simplify sample preparation without compromising data using the Attune Attune/Attune NxT flow cytometer/flow cytometry, flow cytometry sample preparation,immunophenotyping
BioProbes article (2011) A practical guide to immunocytochemistry—Optimized antibody labeling and detection antibodies, antibody labeling 
BioProbes article (2011) New simplified workflow options for maximum-yield protein conjugations—From APEX to Zenon: Molecular Probes protein labeling kits Alexa Fluor, antibodies, antibody labeling, flow cytometry/flow cytometer, immunofluorescence (IF), protein labeling, Zenon
BioProbes article (2011) Focusing on the FAK signaling pathway—Phosphorylation-specific antibodies for Focal Adhesion Kinase (FAK) antibodies, cell signaling, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC)
BioProbes article (2012) Breakthrough tools for epigenetics studies—Structural Genomics Consortium and Life Technologies collaborate on first antibody master set antibodies, cell structure-nucleus, ChIP, epigenetics
BioProbes article (2012) When inflammatory cytokines are unbalanced—Investigate the role of inflammation in disease states using immunoassays antibodies, cancer, ELISA, inflammation, multiplex ELISA/Luminex
BioProbes article (2012) Automating cell biology—Create your own HCS assay using trusted Molecular Probes dyes antibodies, antibody labeling, cell structure, cell structure-actin, cell structure-lysosomes, cell structure-mitochondria, CellLight, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2012) Akt signaling pathway: The master regulator in human disease—Dissect the Akt Pathway Using ABfinity Antibodies, Luminex Multiplex Assays, and More antibodies, cell signaling, ELISA, Luminex, multiplex ELISA/Luminex
BioProbes article (2012) Recombinant antibodies: A new gold standard ABfinity, antibodies 
BioProbes article (2012) A new application for pHrodo dextrans antibodies, antibody labeling, Click-iT, flow cytometer/flow cytometry, dextrans, fluorescence particles, protein labeling, Qdot
BioProbes article (2013) Beyond Lights Limits: Fluorescence imaging at the nanoscale—Fluorescent probes for three super-resolution modalities antibodies, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, SIM, STORM, STED, super resolution microscopy
BioProbes article (2013) Expand your multicolor capacity with the Pacific Green dye—New violet laser–excitable Pacific Green dye and conjugates for flow cytometry flow cytometer/flow cytometry, immunophenotyping, violet-laser excited dyes and reagents
BioProbes article (2013) Target key proteins in cell signaling pathways—ABfinity recombinant antibodies for cancer research ABfinity antibodies, antibodies, cancer, cell signaling, recombinant antibodies
BioProbes article (2014) Obtain the highest-quality data your system can deliver—Fluorescent microspheres for calibrating microscopes and flow cytometers calibration, cell counting, flow cytometer/flow cytometry, flow cytometer set up and calibration, fluorescence microscopy/fluorescence imaging, immunophenotyping, microscope calibration, particles
BioProbes article (2014) The next generation in acoustic focusing cytometry is here—The Attune NxT Acoustic Focusing Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence
BioProbes article (2015) Applications for therapeutic monoclonal antibodies—Tools for studying antibody internalization and trafficking antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2015) Breathe new life into hypoxia research—Intracellular detection of low oxygen tension in live cells antibodies EVOS, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging
BioProbes article (2015) No-wash, no-lyse assays for the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry,immunophenotyping, multicolor flow cytometry
BioProbes article (2015) No-compensation immunophenotyping using a four-laser flow cytometer Attune/Attune NxT, flow cytometer/flow cytometry,immunophenotyping, multicolor flow cytometry
BioProbes article (2015) Flow cytometry panel design: The basics Attune/Attune NxT, flow cytometer/flow cytometry,immunophenotyping, multicolor flow cytometry
BioProbes article (2015) Isolation and characterization of exosomes using magnetic beads antibodies, exosomes, flow cytometer/flow cytometry,immunophenotyping, multiplex ELISA/Luminex,  particles, protein blotting, western detection
BioProbes article (2015) Journal Club—Multiparameter detection of early apoptosis markers without compensation apoptosis, Attune/Attune NxT, cell structure-mitochondria, flow cytometer/flow cytometryimmunophenotyping
BioProbes article (2015) The next generation in flow cytometry—The Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence
BioProbes article (2016) Optimized multicolor immunofluorescence panels (OMIPs)—Jump-start your experimental design with published antibody and reagent panels Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
BioProbes article (2016) Transcription factor expression during differentiation of hPSC-derived cardiomyocytes—A multiparametric approach using the Attune NxT Flow Cytometer Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry
BioProbes article (2016) A complete suite of reagents, from Cas9 delivery tools to cell function assays—The CRISPR-Cas9 system for genome editing western blotting, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2016) Histological analysis using the CellInsight CX7 HCA Platform—Quantitative imaging of histological samples CellInsight, high content analysis, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC)
BioProbes article (2016) Antibodies for pluriptent stem cells and neural lineage cells—Light up neural diffentiation pathways antibodies, high content analysis, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunophenotyping, neuroscience, stem cell research
BioProbes article (2016) Antibodies specific for misfolded proteins associated with neurodegeneration—Protein misfolding in neurodegenerative diseases antibodies, neuroscicence
BioProbes article (2016) Superclonal secondary antibodies for sensitive and reproducible immunoassays—Optimize immunodetection with recombinant secondary antibodies Alexa Fluor, antibodies, recombinant antibodies
BioProbes article (2016) Click-iT EdU and Click-iT TUNEL Colorimetric Assays for immunohistochemistry—Proliferating and apoptotic cells revealed apoptosis, brightfield microscopy, cell proliferation, Click-iT, colorimetric,fixed-cell imaging, immunohistochemistry (IHC)
BioProbes article (2016) Antibody-based tools for identifying cell types and proteins of Interest—Cells and soluble mediators of the tumor microenvironment antibodies, cancer, ELISA, immunology, multiplex ELISA/Luminex
BioProbes article (2017) Double vision: Simultaneous visualization of protein and RNA targets—ViewRNA Cell Plus Assay for antibody labeling and in situ hybridization in individual cells antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes, in situ hybridization,ViewRNA
BioProbes article (2017) T lymphocyte immunophenotyping—14-color flow cytometry panel featuring Super Bright antibody conjugates antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, immunology, immunophenotyping, multicolor flow cytometry, Super Bright
BioProbes article (2017) Boost your stem cell antibody arsenal—Monoclonal antibodies for studying human pluripotent stem cells antibodies, immunofluorescence (IF), pluripotent stem cells, stem cell research
BioProbes article (2017) Appetite for destruction—Studying macroautophagy with Premo fluorescent protein–based sensors autophagy, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, immunocytochemistry (ICC), immunofluorescence (IF), live-cell imaging
BioProbes article (2017) Effectively monitor antibody internalization and trafficking—Improved labeling tools for antibody–drug conjugate screening and characterization antibodies, antibody-drug conjugates, antibody labeling, drug research, endocytosis/phagocytosis, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC), internalization, pH detection
BioProbes article (2017) Bright fluorescent polymer dyes for the violet laser—Super Bright antibody conjugates antibodies, flow cytometer/flow cytometry, fluorescent dyes, Super Bright
BioProbes article (2017) Amplify signals for fluorescence imaging—SuperBoost kits with Alexa Fluor tyramides for signal amplification Alexa Fluor, antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary detection, signal amplification
BioProbes article (2017) Harness immune checkpoints to combat tumors—Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays antibodies, flow cytometer/flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC), immunology, immuno-oncology
BioProbes article (2017) Powering up drug discovery—Cancer biology applications using the Attune NxT Flow Cytometer antibodies, Attune/Attune NxT, cancer, drug discovery, flow cytometer/flow cytometry, fluorescent dyes
BioProbes article (2017) Comprehensive strategy for antibody validation—Characterization of antibody performance using immunoprecipitation and mass spectrometry antibodies, antibody specificity, antibody validation, immunoprecipitation (IP), mass spectrometer/mass spectrometry
BioProbes article (2017) Examine signaling pathways with targeted proteomics—Quantitative analysis of the AKT/mTOR pathway using multiplex immunoprecipitation and targeted mass spectrometry antibodies, antibody specificity, cell signaling, immunoprecipitation (IP), mass spectrometer/mass spectrometry
Molecular Probes Handbook TSA and other peroxidase-based signal amplification techniques—Section 6.2 Alexa Fluor, antibodies, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), reagents, secondary detection, signal amplification
Molecular Probes Handbook Qdot nanocrystals—Section 6.6 avidins and streptavidins, cell tracking, fluorescence, particles, Qdot, secondary antibodies
Molecular Probes Handbook Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon
Molecular Probes Handbook Secondary immunoreagents—Section 7.2 antibodies, avidins and streptavidins, secondary antibodies
Pathway poster (2012) TNF signaling pathway  antibodies, cell signaling, immunoassays
Pathway poster (2012) Toll-like receptor signaling pathway  antibodies, cell signaling, immunoassays
Pathway poster (2012) The human kinome  antibodies, cell signaling, immunoassays
Pathway poster (2012) Integrin signaling pathway  antibodies, cell signaling, immunoassays
Pathway poster (2012) Interferon signaling pathway  antibodies, cell signaling, immunoassays
Pathway poster (2012) MAPK signaling pathway  antibodies, cell signaling, immunoassays
Pathway poster (2012) Akt/mTOR signaling pathway  antibodies, cell signaling, immunoassays
Pathway poster (2012) Jak/Stat signaling pathway  antibodies, cell signaling, immunoassays
Protocol Immunofluorescent staining of intracellular antigens on cultured cells fluorescence microscopy/fluorescence imaging, general ab staining, imaging, immunofluorescence (IF)
Protocol Immunohistochemical staining of formalin-fixed paraffin-embedded tissues general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Indirect method (purified) general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Indirect method (biotin) general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC frozen tissue—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE Tissue Trypsin Digestion—Direct Method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue trypsin digestion antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue low pH antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue low pH antigen retrieval—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue high pH antigen retrieval—Indirect method general ab staining, imaging, immunohistochemistry (IHC)
Protocol IHC FFPE tissue high pH antigen retrieval—Direct method general ab staining, imaging, immunohistochemistry (IHC)
Scientific poster (2007) Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry  antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2007) Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis  Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2007) Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay  Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents  Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2008) Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants  Alexa Fluor, antibodies, cell signaling, flow cytometer/flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon
Scientific poster (2008) Novel Click-IT TUNEL Assay for detection of cell death  Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability
Scientific poster (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry  Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008) Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method  Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling
Scientific poster (2009) A comparative study of 25 different CD4 conjugates  Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot
Scientific poster (2009) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Scientific poster (2009) Dead cell stains in flow cytometry: A comprehensive analysis  apoptosis, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, viability
Scientific poster (2009) Qdot nanocrystal conjugates in multispectral flow cytometry  Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents
Scientific poster (2009) Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging  Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2010) Acoustic flow cytometry for multicolor immunophenotyping analysis  antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents
Scientific poster (2010) Acoustic focusing cytometry: Sensitivity and throughput  apoptosis, Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis
Scientific poster (2010) High content imaging of autophagy  antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader
Scientific poster (2010) Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010) Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis  Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) Use of surface-modified microspheres for setting compensation in multicolor flow cytometry  antibodies, flow cytometer/flow cytometry, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents
Scientific poster (2010) ABfinity antibodies: A novel approach to antibody development  Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, immunocytochemistry (ICC), western blotting
Scientific poster (2010) A comparison of three techniques to induce efficient ex vivo T cell expansion  Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010) Identification and characterization of O-GlcNAc modification of galectin-1 in mesenchymal stem cells using click chemistry  Alexa Fluor, BacMam technology, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, immunocytochemistry (ICC), protein enrichment, protein labeling, western blotting
Scientific poster (2010) Rare event detection using acoustic cytometry  Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, rare-event detection
Scientific poster (2011) Acoustic flow cytometry for multicolor immunophenotyping analysis  antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents
Scientific poster (2011) No-lyse no-wash immunophenotyping using acoustic cytometry  Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, nanocrystals, Qdot
Scientific poster (2011) Acoustic cytometry for rare event detection of PNH cells  Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, rare-event detection
Scientific poster (2011) Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2011) Validation of high content imaging and analysis assays for cell health and cytotoxicity  antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011) The next step in the analysis of lymphocyte proliferation  Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents
Scientific poster (2011) Flash & Glow 1,2-dioxetane chemiluminescent substrate formulations for clinical research platforms  antibodies, chemiluminescence, ELISA, enzyme activity detection, magnetic beads, microplate reader, substrate comparison
Scientific poster (2011) The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Site-specific click chemistry-mediated labeling of antibody glycans using metabolic and enzymatic approaches  Alexa Fluor, antibodies, antibody labeling, Click-iT, fluorescent dyes, western blotting
Scientific poster (2012) Studying malaria using the Attune flow cytometer  Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, malaria, parasite identification
Scientific poster (2012) High sensitivity applications for the Applied Biosystems Attune  Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, viability
Scientific poster (2012) A novel no-wash, no-lyse method for residual leukocyte enumeration using acoustic focusing flow cytometry and a cell-membrane permeant DNA dye  Attune/Attune NxT, cell counting, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, rare-event detection, violet laser-excited reagents
Scientific poster (2013) Pacific Green dye: A new fluorophore for violet laser excitation  Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, viability, violet laser-excited reagents
Scientific poster (2013) Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping  Alexa Fluor, Attune/Attune NxT, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping
Scientific poster (2013) Cells coming to life: Tools for visualizing immune response  Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection
Scientific poster (2015) Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2015) A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer  Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, phagocytosis, whole blood
Scientific poster (2016) Correlating internalization and potency to accelerate antibody discovery and development  brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC)
Scientific poster (2016) Cell mediated cytotoxicity in "untouched" whole blood  Attune/Attune NxT, cell concentration, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, whole blood
Scientific poster (2016) No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads  antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry compensation, immunophenotyping, multicolor flow cytometry, particles
Scientific poster (2016) Incorporating histological analysis into existing high content imaging  antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC)
Scientific poster (2016) A microsphere-based universal compensation approach  Attune/Attune NxT, Flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry sample preparation, immunophenotyping, multicolor flow cytometry
Scientific poster (2016) Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Tutorial 1.2 Fixation–Fixed cell imaging: 5 steps for publication-quality images 
The next step of tissue preparation is fixation. Fixation refers to a chemical means of killing and preserving cells in a particular physiological state, and in many cases, to preserve morphology. Proper fixation equals preservation of target.
fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial 4.1 Mounting media–Fixed cell imaging: 5 steps for publication-quality images 
Using the right mounting media can impact your experiment. Be sure to choose the right type of mountant for your set-up.
antifade mounting media, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), mounting media
Tutorial 3.2 Secondary antibody optimization–Fixed cell imaging: 5 steps for publication-quality images 
Secondary antibody detection protocols also need to be optimized for each primary antibody used.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody protocol, secondary detection
Tutorial 4.2 Photobleaching and antifades–Fixed cell imaging: 5 steps for publication-quality images 
What is photobleaching and how can you prevent it from destroying your sample? Options for antifades are discussed.
antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), photobleaching, Prolong Gold, Vectashield
Tutorial 5.5 Ethical considerations–Fixed cell imaging: 5 steps for publication-quality images 
Ethical imaging means trustworthy data, and thus, publishable data. How to treat your samples and data to preserve data integrity is presented.
ethical considerations, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF), sample manipulation
Tutorial 5.2 Imaging platforms-software–Fixed cell imaging: 5 steps for publication-quality images 
Taking images on a microscope usually entails having some type of imaging software that aids in taking the image and assists in combining differing colors into one. There are some very important aspects to keep in mind to get a publishable image.
FiFI software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, ImageJ, imaging software, imaging systems, immunofluorescence (IF)
Tutorial 1.1 Culture conditions–Fixed cell imaging: 5 steps for publication-quality images 
The first step in obtaining a good image is tissue preparation. For cultured cells, the cells must have good cell health and morphology, as well as good confluency. Healthy cells equal healthy data.
EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial 1.5 Autofluorescence–Fixed cell imaging: 5 steps for publication-quality images 
The last step in cell preparation is autofluorescence. Cells and tissue can have a certain degree of autofluorescence that can confuse the specific signal, and lower the signal-to-background. Overcoming autofluorescence means greater sensitivity.
autofluorescence, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), tissue autofluorescence
Tutorial 5.4 Image analysis with Celleste software–Fixed cell imaging: 5 steps for publication-quality images 
The functionality of the Celleste softwis reviewed and considerations for processing the image in different software programs are described.
Celleste software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF)
Tutorial 1.4 Blocking–Fixed cell imaging: 5 steps for publication-quality images 
The next step after permeabilization is blocking, and there are a number of blocking techniques. Protein blocking equals specific antibody binding. Dye charge blocking means less non-specific binding.
dye charge blocking, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), nonspecific binding, protein blocking
Tutorial 2.2 Primary antibody protocol optimization–Fixed cell imaging: 5 steps for publication-quality images 
Every primary antibody must be optimized separately. There are many protocols available, and it is important to understand a "one size fits all" approach gives inferior results, as every antibody is slightly different. Learn how to approach optimization.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies, primary antibody protocol
Tutorial 5.1 Imaging platforms-hardware–Fixed cell imaging: 5 steps for publication-quality images 
The fifth step of the process is the actual imaging. To capture top-quality images, you need an imaging platform with top-of-the-line imaging capabilities. Here we review considerations for getting the best image.
EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial 3.5 Dye choice and special concerns–Fixed cell imaging: 5 steps for publication-quality images 
There are many different dyes spanning the visible, far-red, and infrared wavelengths.Considerations for making the right choices for your experiment are presented.
dye choice, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, Fluorescence SpectraViewer, immunofluorescence (IF), label choice, muliparametric dye selection
Tutorial 1.3 Permeabilization–Fixed cell imaging: 5 steps for publication-quality images 
The next step is the permeabilization of the cells which is the key to opening intracellular compartments.
cell permeabilization, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial 3.4 Controls–Fixed cell imaging: 5 steps for publication-quality images 
Researchers should conduct all necessary controls to rule out the possibility of non-specific binding or non-specific signal. Types of controls are described. Proper controls will boost your confidence in your final results.
fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging controls, immunofluorescence (IF), sample controls
Tutorial 5.3 Image capture with EVOS FL Auto 2.0–Fixed cell imaging: 5 steps for publication-quality images 
Here the advantages of using the EVOS FL Auto 2.0 imaging system to capture your images are discussed.
EVOS FL Auto 2.0, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image capture, immunofluorescence (IF)
Tutorial 3.3 Amplification techniques–Fixed cell imaging: 5 steps for publication-quality images 
If the signal is not strong enough using standard secondary detection schemes, you can increase the signal using amplification techniques. This is particularly important for low-expressing antigens, or rare-cell detection in samples. Examples are discussed.
biotin and streptavidin, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), signal amplification, tyramide detection
Tutorial 2.1 Primary antibody choice–Fixed cell imaging: 5 steps for publication-quality images 
After preparation, the second step to publishable images is to label the sample, usually involving primary antibodies to your specific targets of interest. The antibody source, the use of direct versus labeled antibodies as well as the validation for specific applications is discussed.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies
Tutorial 3.1 Secondary antibody choice–Fixed cell imaging: 5 steps for publication-quality images 
Step three of the five steps in making publishable images is to detect the label. That is, to detect with a secondary antibody, for instance, or an amplification technique, as well as to determine what controls to use. Your options are discussed.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody, secondary detection
Tutorial Labeling a purified antibody: Educational video on how to label your next antibody for imaging or fluorescence 
Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.
antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF)
Video How to site specifically label your antibody using SiteClick technology 
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SitClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
Webinar Fixed cell imaging—Five steps for publication-quality image 
With over 40 years dedicated to cell imaging research, we offer long-proven tools and protocols to help confidently create quality cell images the first time. This on demand webinar covers the 5 essential steps to getting great images.
antibodies, blocking, Celleste, dyes, EVOS FL Auto 2.0, fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), sample detection, sample labeling, sample preparation, signal amplification
Webinar Learn to choose the right fluorophore when designing experiments 
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore: How to choose the best organic dye for an assay Quantum dots and how they compare to other dyes When to use a phycobiliprotein like R-PE or APC When to use fluorescent proteins like GFP How to choose a suitable dye to match your instrument In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar A practical approach to antibody labeling 
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will: Provide an overview of our antibody labeling kits Offer guidance on which methods are ideal for specific applications and experiments Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar An introduction to immunofluorescence staining of cultured cells 
In this webinar, we discuss the steps of an immunofluorescent staining protocol including material list, common variations, and necessary controls. We'll also provide a simple troubleshooting guide and examine how to avoid common pitfalls. Presented by Jason Kilgore, Technical Support Specialist, Thermo Fisher Scientific.
antibodies, antibody labeling,antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar Basic techniques in autophagy research 
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)
Webinar Basics of multicolorflow cytometry panel design 
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including: Rules for designing panels Examples and practical application of these rules Controls and standardization Relevance of panel design to new mass cytometry platforms
antibodies, compensation, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry, viability
Webinar A comparison of basic immunofluorescent labeling strategies 
In this free webinar, we will compare different immunofluorescent labeling strategies exploring the pros and cons of each method. You will learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We'll also present a simple decision tree to aid in determining the best method for each situation.
Alexa Fluor, antibodies, antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF)