Technical and educational videos for cell analysis topics

Cell Analysis Educational Videos & Webinars

These videos, tutorials and webinars are designed to provide technical and education information for cell analysis platforms, applications and techniques.

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How the Click-iT Plus EdU Proliferation Assay works

Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that cannot be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.

Webinar—Breathing new life into hypoxia research

Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar we cover:

  • Overview of hypoxia in human diseases
  • Classical methods for setting up hypoxic conditions
  • Novel instruments and reagents for imaging cells in hypoxic conditions

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Tutorial Tutorial: Labeling a purified antibody
Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.
antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Tutorial Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data flow cytometer calibration, flow cytometer/low cytometry, multicolor flow cytometry
Tutorial Molecular Probes Tutorial Series—Anatomy of Fluorescence Spectra absorbance, emission, excitation, spectra
Tutorial Molecular Probes Tutorial Series—Introduction to Flow Cytometry flow cytometer calibration, flow cytometer/flow cytometry, multicolor flow cytometry
Tutorial Molecular Probes Tutorial Series—Introduction to Fluorescence fluorescence
Tutorial Molecular Probes Tutorial Series—Overview of Filters and Light Sources emission, excitation, filters, light cubes, light sources
Tutorial HCA Q&A: Can I use my microscope images in your HCA software to do analysis? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: Is there a difference between high-content analysis and all of the other names it’s called? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: When is confocality useful in high-content analysis? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: What is the difference between an imaging plate reader and a high-content platform? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: Can I use your software with images I have taken from other instruments? Can the portability of your software help me import and export data and images easily? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: How is Thermo Scientific HCS Studio™ 2.0 Software flexible and what does 'flexible software' exactly mean? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: I don't have a dedicated resource to operate and maintain a high-content system. Can you tell me how difficult it is to operate high-content technology? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: What is Thermo Scientific HCS Studio™ 2.0 Software and what can it do? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: I get a lot of data from HCA but I don't know what to do with it. What measurements should I be looking at to describe what is happening in my biology? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: How has Thermo Scientific made high-content software easy to learn, and easy to use? ArrayScan, CellInsight, high content analysis
Tutorial HCA Q&A: Why do you include near-IR capabilities in your products? ArrayScan, CellInsight, high content analysis
Video Mounting a Coverslip – Molecular Probes School of Fluorescence
Molecular Probes School of Fluorescence explains step-by-step how to mount a coverslip properly for cellular imaging. Formulations of mounting media that can add favorable properties such as optimizing the refractive index to match that of glass, preventing photobleaching, or preserving samples for long-term storage are widely available.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
Video Wash a coverslip– Molecular Probes School of Fluorescence
Molecular Probes School of Fluorescence explains the wash step common to many cellular imaging protocols.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
Video CellEvent Caspase 3/7 reagent and apoptosis detection
HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal.
apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video CellTracker Violet reagent and mitosis
U-2 OS cells were transduced with CellLight Tubulin-GFP and Cellular Lights Actin-RFP. The following day cells were labeled with 5uM CellTracker Violet BMQC for 30 minutes at 37C in complete media and washed in fresh media. Images were taken every 5 minutes for 16 hours.
cell structure-all, cell tracking, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Click-iT Plus EdU Proliferation Assay
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging
Video Click-iT Plus TUNEL apoptosis assays
The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed “click” reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP.
apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging
Video Compilation of live cell imaging videos using Invitrogen fluorescent reagents
This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes.
cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video Detection of oxidative stress with CellROX Green in U2-OS cells
U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA.
CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video How to site specifically label your antibody using SiteClick technology
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SitClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
Video LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Mitochondrial dynamics through cell division
U2OS cells were transduced with CellLight Mito-RFP and imaged every 5 minutes for 16 hours. Extensive mitochondrial motility is seen throughout mitosis and as the cell regains it's pre mitotic shape following mitosis.
cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Time-lapse of cell migration and apoptosis during angiogenesis
Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator.
angiogenesis, apoptosis, EVOS, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator
Video Time-lapse with Qtracker 655 cell labeling reagent
Time-lapse image of live cells labeled with Qtracker reagents. Adherent HeLa cells were incubated with Qtracker VIVID 655 Cell Labeling Kit (Cat. No.Q25021MP) at 10 nM for one hour. Images were collected over 90 min using a Zeiss LSM 710 confocal microscope and are shown pseudo-colored magenta.
fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Viability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green)
HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Stauroporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead).
cell structure-nucleus, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Wound healing and cadherin-4 expression
Murine glioma cells were transfected with a DsRed-cadherin-4 fusion, which plays a critical role in cell migration during wound repair, or a GFP control plasmid, then mixed with wild-type cells and seeded in a 24-well plate. After scratch wounding, cell migration was followed over a 21 h period on the EVOS Cell Imaging System equipped with an EVOS Onstage Incubator using a 10X phase objective. Images were captured at an interval of about 10 minutes (116 images total). Video courtesy of Paolo Malatesta, PhD., IRCCS Azienda Ospedaliera Universitaria San Martino -- IST, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
EVOS, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, wound healing
Video Z Stack image of HeLa cells labeled with CellLights reagents
A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm.
cell health, cell structure-all, CellLight, EVOS, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging
Video The two worlds of cell separation cell expansion, cell isolation, Dynabeads, magnetic beads
Video Miltenyi artifacts in T-cells after 72 hours cell expansion, cell isolation, Dynabeads, magnetic beads
Video Exosomes—The Next Small Thing: Episode 1—What is an exosome? Exosomes
Video Exosomes—The Next Small Thing: Episode 2—The history and promise of an exosome Exosomes
Video Exosomes—The Next Small Thing: Episode 3—Exosomes in cancer research Exosomes
Video Exosomes—The Next Small Thing: Episode 4—Curiosity and a passion for science Exosomes
Video Exosomes—The Next Small Thing: Episode 5—Collaboration—The key to scientific success Exosomes
Video Exosomes—The Next Small Thing: Episode 6—Exosomes the next small thing Exosomes
Webinar The meaning of life at the cellular level: Probing viability with fluorescence
This webinar will provide an overview of features of healthy and unhealthy cells as well as describing key parameters that can be measured to assay cell viability. The webinar also offers a comprehensive guide to available labeling and detection technologies for cell health research as well as tips and tricks on how to best use them.
cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, viability
Webinar A comparison of basic immunofluorescent labeling strategies
In this free webinar, we will compare different immunofluorescent labeling strategies exploring the pros and cons of each method. You will learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We’ll also present a simple decision tree to aid in determining the best method for each situation.
Alexa Fluor, antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF)
Webinar A practical approach to antibody labeling
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
  • Provide an overview of our antibody labeling kits
  • Offer guidance on which methods are ideal for specific applications and experiments
  • Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar An introduction to immunofluorescence staining of cultured cells
In this webinar, we discuss the steps of an immunofluorescent staining protocol including material list, common variations, and necessary controls. We’ll also provide a simple troubleshooting guide and examine how to avoid common pitfalls. Presented by Jason Kilgore, Technical Support Specialist, Thermo Fisher Scientific.
antibodies, antibody labeling, Antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar Basic techniques in autophagy research
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include:
  • Tips and tricks for selecting the right tools and achieving the best results
  • Fluorescent proteins and antibodies used to analyze both live and fixed cells
  • Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis
Webinar Basics of flow cytometry, Part I: Gating and data analysis
An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of flow cytometry, Part II: Compensation
This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation.
flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of multicolorflow cytometry panel design
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
  • Rules for designing panels
  • Examples and practical application of these rules
  • Controls and standardization
  • Relevance of panel design to new mass cytometry platforms
antibodies, compensation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar Breathing new life into hypoxia research
Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss:
  • Overview of hypoxia in human diseases
  • Classical methods for setting up hypoxic conditions
  • Novel instruments and reagents for imaging cells in hypoxic conditions
EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator
Webinar DNA content cell cycle analysis using flow cytometry
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss:
  • An overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content
  • Selection of DNA dyes for live cell and fixed cell analysis
  • Tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometer/flow cytometry
Webinar Flow cytometry in microbiological research
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
flow cytometer/flow cytometry, microbiology, multicolor flow cytometry, viability
Webinar Introduction to basic cytoskeleton labeling and detection
The cytoskeleton is a key component of mammalian cells, providing the framework for cell migration and intracellular transport, furthermore the cytoskeleton regulates cell size and shape as well as important processes such as mitosis and endocytosis. We offer a number of solutions for researchers using fluorescent probes to study the cytoskeleton. This webinar will provide an overview of the structures that comprise the cytoskeleton and important experimental parameters. The webinar also offers a comprehensive guide to available labeling and detection technologies for cytoskeletal research as well as tips and tricks on how to best use them. These tools include those for live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform.
cell structure-actin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar Learn to choose the right fluorophore when designing experiments
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore:
  • How to choose the best organic dye for an assay
  • Quantum dots and how they compare to other dyes
  • When to use a phycobiliprotein like R-PE or APC
  • When to use fluorescent proteins like GFP
  • How to choose a suitable dye to match your instrument
In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar The meaning of life at the cellular level: detecting apoptosis with fluorescence
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes:
  • An overview of the features of apoptosis
  • Key parameters that can be measured to assay apoptosis
  • A comprehensive guide to available labeling and detection technologies for apoptosis research
  • Tips and tricks to best implement those technologies
apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins
Webinar The meaning of life at the cellular level: Visualizing membrane trafficking
Membrane trafficking underlies the acquisition and release of cellular material as well as the transport of macromolecules from the site of cellular synthesis to their functional location. Thermo Fisher Scientific offers a number of solutions for researchers using fluorescent probes to study key components of the endocytotic and secretory pathway. This webinar will:
  • Provide an overview of the processes involved in membrane trafficking, along with strategies to investigate them using fluorescence microscopy
  • Offer a comprehensive guide to available labeling and detection technologies for membrane trafficking research
  • Provide tips and tricks on how to best implement those technologies
fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, membrane trafficking, particles
Webinar From the hood to the microscope; Revolutionizing cell-based imaging
Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include:
  • Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy
  • Label cells to maximize signal-to-noise for fluorescence imaging
  • Prepare culture conditions and capture images without losing temporal data for time-lapse imaging
  • Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures
cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator