High-Content Analysis (Quantitative Imaging) Information

High-content analysis, also called quantitative imaging, is a process where automated microscopy is combined with multi-parametric imaging. Visualization software provides quantitative data about cell populations. In this section you will find application notes, video tutorials, question and answer videos as well as webinars all designed to help you better understand what high-content analysis is all about and how to get the most out of your data.

High-content analysis (quantitative imaging) features

BioProbes article

HCA platforms for automated, quantitative analyses

A wealth of information about a physiological process or pathological condition can be collected by monitoring the localization and abundance of specific proteins using fluorescence imaging but quantifying these essential processes is time consuming and laborious. High-content analysis can remove these obstacles, resulting in robust and reproducible research.

On-demand webinar

Breathing new life into hypoxia research

In this webinar, we will discuss hypoxia in human diseases, classical methods for setting up hypoxic conditions, and novel instruments and reagents for imaging cells in hypoxic conditions.


High-content analysis (quantitative imaging) learning resources

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Type Title Categories
Application note (2012) Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening  ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability
Application note (2013) Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell colonies  Alexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research
Application note (2013) Multiplexed mitosis and apoptosis analysis  antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles
Application note (2014) Using a high content analysis database as a repository for data  database management, drug discovery, high content analysis, microplate reader
Application note (2014) High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry  ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Application note (2014) Oxidative stress measurements made easy: CellROX reagents with simple workflow for high content imaging  ArrayScan, CellInsight, CellROX, high content analysis, oxidative stress
Application note (2014) Elucidating the mechanism of cell death through the use of high content analysis  apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Application note (2015) Quantitation of proliferating cells with the EVOS FL Auto Imaging System antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis
BioProbes article (2006) Reagents and assays for high-content screening  cell structure-nucleus, cell structure-plasma membrane, fluorescent dyes, high content analysis, fluorescence microscopy/fluorescence imaging
BioProbes article (2007) Mercator multiplex phosphoantibody-based array  antibody labeling, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, protein labeling, protein quantitation
BioProbes article (2007) Improved results are just a click away  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides
BioProbes article (2007) New applications for Vybrant DyeCycle stains  Alexa Fluor, cell cycle, cell isolation, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, multicolor flow cytometry, stem cell research
BioProbes article (2007) A new approach to cell-based multiplexing expands the drug screening capabilities of flow cytometry  Alexa Fluor, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, sample preparation
BioProbes article (2007) Following differentiation into adipocytes  cell differentiation, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, stem cell research
BioProbes article (2008) The picture of cell health  Alexa Fluor, cell health, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, nucleic acid quantitation, nucleosides, reagents
BioProbes article (2008) A no-wash indicator for high-throughput calcium detection  BacMam technology, cell signaling, drug discovery, flow cytometer/flow cytometry, fluorescent proteins, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2008) Click chemistry: Making biodiscovery a snap  Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, nucleosides
BioProbes article (2008) Seeing clearly: Accurate cell identification—CellMask stains for fluorescence-based imaging  cell structure-cytoplasm, cell structure-nucleus, cell structure-plasma membrane, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging
BioProbes article (2008) Measuring mitochondrial protein levels and enzyme activity—Get both readouts with MitoProfile dipstick and microplate assays  enzyme activity detection, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, protein detection
BioProbes article (2009) Multiparametric approaches for predictive hepatotoxicity  cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, viability
BioProbes article (2009) Innovative solutions for ion channel research  BacMam technology, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2009) RNA as you've never seen it before  Alexa Fluor, Click-iT, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, nucleic acid detection, nucleosides
BioProbes article (2009) Live-cell imaging of cell cycle and cell division  BacMam technology, cell cycle, flow cytometer/flow cytometry, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging
BioProbes article (2009) High-content analysis of cytotoxicity  cell health, cell proliferation, Click-iT, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader, viability
BioProbes article (2009) Faster, easier calcium flux assays  cell signaling, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, microplate reader
BioProbes article (2009) Cytotoxicity and cell proliferation studies—The CyQUANT Direct Cell Proliferation Assay  cell health, cell proliferation, fluorescent dyes, high content analysis, microplate reader, viability
BioProbes article (2009) High-throughput chloride channel screening  BacMam technology, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators
BioProbes article (2009) TUNEL vision  apoptosis, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, sample preparation, sample stability
BioProbes article (2009) Fluorescence technologies for high-content imaging and analysis  Alexa Fluor, cell health, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress, protein detection, viability
BioProbes article (2010) Putting autophagy into context autophagy, CellInsight fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2010) Biology in context—Transforming live-cell microscopy with CellLight reagents cell structure, CellLight fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, live-cell imaging
BioProbes article (2010) Choosing the right dye for imaging intracellular calcium calcium flux, fluorescence microscopy/fluorescence imaging, high content analysis, ion channels, ion flux, ion indicators, reagents, sample preparation
BioProbes article (2010) Probes for visualizing secretory transport and autophagy autophagy, cell structure, cell structure-ER, cell structure-golgi, cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) Cells under stress—Fluorescence-based tools for investigating autophagy apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) Alexa Fluor dyes shed light on a new actin assembly factor cell structure-actin, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2011) Neurons in living color—BacMam 2.0 technology and other imaging tools for live-cell studies CellLight, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, live-cell imaging, neuroscience
BioProbes article (2011) Introducing the FLoid Cell Imaging Station—From vessel to image in two clicks FLoid, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2011) Detection of actin, intermediate filaments, and tubulin—Imaging a dynamic cellular scaffold cell structure, cell structure-actin, CellLight fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2011) Bringing cell death to light—CellEvent Caspase-3/7 Substrate and the multiplex analysis of apoptosis apoptosis, ArrayScan, CellEvent, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
BioProbes article (2011) Advances in high-content screening—Assays for multiparametric analysis of cell health ArrayScan, cell health, cell proliferation, cell structure-plasma membrane, Click-iT EdU, fluorescence microscopy/fluorescence imaging, high content analysis, viability
BioProbes article (2011) Explore the powerhouse—Probes for mitochondrial morphology and function cell structure, cell structure-mitochondria CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2011) Endocytosis in disease and cell health cell health, cell structure, CellLight, endocytosis and phagocytosis, fluorescence microscopy/fluorescence imaging, high content analysis, pH detection
BioProbes article (2011) Shedding light on oxidative stress—CellROX Deep Red Reagent for ROS detection ArrayScan, cell health, CellROX, fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2011) No-wash imaging of apoptosis—CellEvent Caspase-3/7 Green Detection Reagent for apoptosis apoptosis, ArrayScan, caspase substrates, CellEvent, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2012) Keeping an eye on the messenger fluorescence microscopy/fluorescence imaging, fluorometer, high content analysis nucleic acid quantitation, Qubit
BioProbes article (2012) On-the-spot imaging and processing EVOS, brightfield microscopy, cell structure-actin fluorescent proteins, cell structure-nucleus, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2012) Ready-to-use reagents shift the focus to results cell structure-nucleus, fluorescence microscopy/fluorescence imaging, high content analysis, sample preparation, viability
BioProbes article (2012) Automating cell biology—Create your own HCS assay using trusted Molecular Probes dyes antibodies, antibody labeling, cell structure, cell structure-actin, cell structure-lysosomes, cell structure-mitochondria, CellLight, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2013) ReadyProbes ready-to-use imaging reagents for brilliant results apoptosis, cell structure-all, fluorescence microscopy/fluorescence imaging, high content analysis, reagents, Imaging sample preparation, viability
BioProbes article (2013) Autophagy made visible—Advanced fluorescent probes for imaging distinct processes in the autophagy pathway autophagy, cell structure, cell structure-lysosomes, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2014) Image live cells through time and space—Optimizing conditions for time-lapse fluorescence microscopy cell structure, cell tracking, CellLight, EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, live-cell imaging, oxidative stress
BioProbes article (2015) High-content analysis applied to autophagy—Automating cell imaging with Thermo Scientific HCA systems ArrayScan, autophagy, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) Multiplexable Click-iT Plus TUNEL assays for in situ apoptosis detection—Visualize apoptosis in context apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) CellInsight CX7 High Content Analysis Platform—Accessible technology for high-content analysis ArrayScan, brightfield microscopy, cell structure CellInsight, high content analysis
BioProbes article (2016) A complete suite of reagents, from Cas9 delivery tools to cell function assays—The CRISPR-Cas9 system for genome editing western blotting, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2016) Histological analysis using the CellInsight CX7 HCA Platform—Quantitative imaging of histological samples CellInsight, high content analysis, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC)
BioProbes article (2016) Antibodies for pluriptent stem cells and neural lineage cells—Light up neural diffentiation pathways antibodies, high content analysis, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunophenotyping, neuroscience, stem cell research
BioProbes article (2017) Fueling discovery with high-content imaging and analysis fuel discovery—Thermo Scientific HCA platforms for automated, quantitative analyses high content analysis,  iPSCs, cell viability and function, CellInsight, drug research, neurite outgrowth, neuroscience, quantitative imaging
Protocol Pharmacological induction of apoptosis with camptothecin cell vitality, flow cytometry, high content analysis, imaging, microplate
Protocol Hoechst 33342 for HCA instruments cell structure, high content analysis
Protocol HCS NuclearMask Red Stain cell structure, high content analysis
Protocol HCS NuclearMask Deep Red Stain cell structure, high content analysis
Protocol HCS NuclearMask Blue Stain cell structure, high content analysis
Protocol HCS Mitochondrial Health Kit cell viability, high content analysis
Protocol HCS LIVE/DEAD Green Kit using Hoechst 33342 cell viability, high content analysis
Protocol HCS LIVE/DEAD Green Kit using HCS NuclearMask Deep Red cell viability, high content analysis
Protocol HCS CellMask Stains cell structure, high content analysis
Protocol DAPI for HCA instruments cell structure, high content analysis
Protocol Click-iT EdU HCS assays cell proliferation, imaging
Scientific poster (2006) LipidTOX dyes for adipocyte staining in routine imaging applications  cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability
Scientific poster (2006) Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations  Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2006) New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism  ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability
Scientific poster (2006) Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer  Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents
Scientific poster (2007) Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry  antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2007) A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry  ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007) Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis  Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2007) Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay  Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling
Scientific poster (2007) BacMam gene delivery of targeted fluorescent proteins, Organelle Lights for HCS  BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, live-cell imaging, nanocrystals, Qdot
Scientific poster (2008) Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization  in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue
Scientific poster (2008) Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents  Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2008) Cell-based potassium ion channel screening with the FluxOR Assay  BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008) Novel Click-IT TUNEL Assay for detection of cell death  Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability
Scientific poster (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry  Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008) FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets  BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008) ThiolTracker Violet dye, a new 405 nm excitable fluorescent probe for detecting intracellular thiols in imaging-based assays  ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, glutathione, high content analysis, microplate reader, protein labeling, violet laser-excited reagents
Scientific poster (2008) A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays  ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, thiol modification, violet laser-excited reagents
Scientific poster (2008) Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method  Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling
Scientific poster (2009) Cell-based assays for predictive hepatotoxicity measurements using high content imaging  Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability
Scientific poster (2009) A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry  Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection
Scientific poster (2009) Monitoring mitotic cells and DNA content by automated imaging and analysis  Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009) High content imaging of endocytosis & phagocytosis using pHrodo conjugates  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2009) Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy  Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability
Scientific poster (2009) High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry  Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability
Scientific poster (2009) High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells  cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability
Scientific poster (2009) Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging  Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009) Cell-based potassium ion channel screening with BacMam using the FluxOR Assay  BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2009) Click chemistry-based assays for fluorescence microscopy and high content imaging  Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability
Scientific poster (2010) High content imaging of autophagy  antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader
Scientific poster (2010) Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs  Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment
Scientific poster (2010) Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) Novel tools to enable high resolution gene expression by investigating the nascent transcriptome  Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability
Scientific poster (2010) Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis  Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) A new generation of cellular imaging probes for neurosciences research  autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010) NA-Fluor Influenza Neuraminidase Assay Kit: Standardization of the classic MUNANA-based neuraminidase activity assay for monitoring antiviral resistance in influenza  drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy  apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress
Scientific poster (2010) The NA-XTD Influenza Neuraminidase Assay Kit: Extended-glow chemiluminescence assay system for highly sensitive influenza neuraminidase assays  chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2010) BacMam gene expression for functional and imaging applications  BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
Scientific poster (2011) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability
Scientific poster (2011) Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2011) Validation of high content imaging and analysis assays for cell health and cytotoxicity  antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011) The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) NA-Fluor and NA-XTD Influenza Neuraminidase Assays for neuraminidase quantitation and inhibition assays  chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2012) Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy  flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability
Scientific poster (2013) Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health  apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific poster (2013) Illuminating endocytosis with targeted pH-sensitive fluorescent compounds  Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2014) New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy  autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader
Scientific poster (2015) Visualizing the life and death of cells: novel probes and platforms  apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
Scientific poster (2016) High content screening in MCF7 and MDA-MB231 cells show differential responses depending on oxygen levels and mechanistic readout for viability cell health, cell viability and function, drug discovery, high content analysis, high throughput screening
Scientific poster (2016) Incorporating histological analysis into existing high content imaging  antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC)
Scientific poster (2017) Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging  EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, live-cell imaging, onstage incubator
Tutorial HCA Q & A 
A video series that answers common questions about high content analysis and the different Thermo Scientific high content imaging platforms
ArrayScan, CellInsight, high content analysis
Webinar Basic techniques in autophagy research 
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)
Webinar Breathing new life into hypoxia research 
Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions
EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator