Transmitted Light/Colorimetric Imaging Information

Colorimetric imaging can be a useful tool for studying highly autofluorescent tissue, such as heart, because this autofluorescence can significantly interfere with a fluorescent probe signal. Colorimetric detection is also favored when labeling must be compatible with standard histological stains that provide morphological and contextual information. Here we have a collection of educational resources to help you get the best results.

Transmitted light/colorimetric imaging features

BioProbes article

Click-iT EdU and Click-iT TUNEL colorimetric assays for immunohistochemistry

This article describes the conversion of our popular fluorescence-based click chemistry assays for cell proliferation and apoptosis to colorimetric assays.

Scientific poster

Incorporating histological analysis into existing high-content imaging

To address the need for quantitative colorimetric imaging, we have developed a method for analyzing histological samples on a high-content imaging instrument using a specialized bright-field unit. 


Transmitted light/colorimetric imaging learning resources

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Type Title Categories
Application note (2016) Blood cell counting using the Countess II FL Automated Cell Counter automated cell counter, brightfield microscopy, cell counting, cell counting sample preparation, Countess, EVOS, fluorescent dyes, immunophenotyping, ReadyProbes
BioProbes article (2007) in situ hybridization made easy  Alexa Fluor, brightfield microscopy,  fluorescent dyes, fluorescence microscopy/fluorescence imaging, gene expression, in situhybridization, nucleic acid detection, sample stability
BioProbes article (2013) The EVOS FL Auto Imaging System—Automated imaging. Simplified brightfield microscopy, cell structure-all, EVOS, fluorescence microscopy/fluorescence imaging
BioProbes article (2014) Introducing a three-channel cell counter for your benchtop—The new and improved Countess II FL Automated Cell Counter cell counting, automated cell counter, brightfield microscopy, Countess, EVOS fluorescence microscopy/fluorescence imaging
BioProbes article (2015) CellInsight CX7 High Content Analysis Platform—Accessible technology for high-content analysis ArrayScan, brightfield microscopy, cell structure CellInsight, high content analysis
BioProbes article (2016) Click-iT EdU and Click-iT TUNEL Colorimetric Assays for immunohistochemistry—Proliferating and apoptotic cells revealed apoptosis, brightfield microscopy, cell proliferation, Click-iT, colorimetric,fixed-cell imaging, immunohistochemistry (IHC)
Protocol Colorimetric FFPE—trypsin digestion colorimetric staining, general ab staining, imaging
Protocol Colorimetric FFPE—Low pH antigen retrieval colorimetric staining, general ab staining, imaging
Protocol Colorimetric FFPE—High pH antigen retrieval colorimetric staining, general ab staining, imaging
Scientific poster (2008) Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization  in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue
Scientific poster (2015) Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2016) Correlating internalization and potency to accelerate antibody discovery and development brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC)
Scientific poster (2016) Incorporating histological analysis into existing high content imaging  antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC)
Scientific poster (2016) Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy  apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
White paper (2015) Accuracy and precision with the Countess II FL Automated Cell Counter automated cell counter, brightfield microscopy, cell counting, Countess