Autophagy Information

This section features educational webinars, application notes, articles and more for studying the process of autophagy.

Autophagy features

Scientific poster

Scientific poster

New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy

Here we describe recently developed tools to study mechanisms of cargo transport to the lysosome: endocytosis and autophagy, as well as novel tools to examine lysosomal structure and function.

On-demand webinar

Basic techniques in autophagy research

This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. 


Autophagy learning resources

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Type Title Categories
Application note (2014) Elucidating the mechanism of cell death through the use of high content analysis  apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
BioProbes article (2010) Putting autophagy into context atophagy, CellInsight fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress
BioProbes article (2010) Probes for visualizing secretory transport and autophagy autophagy, cell structure, cell structure-ER, cell structure-golgi, cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2010) Cells under stress—Fluorescence-based tools for investigating autophagy apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2013) Autophagy made visible—Advanced fluorescent probes for imaging distinct processes in the autophagy pathway autophagy, cell structure, cell structure-lysosomes, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2015) High-content analysis applied to autophagy—Automating cell imaging with Thermo Scientific HCA systems ArrayScan, autophagy, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
BioProbes article (2017) Appetite for destruction—Studying macroautophagy with Premo fluorescent protein–based sensors autophagy, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, immunocytochemistry (ICC), immunofluorescence (IF), live-cell imaging
Molecular Probes Handbook Assays for apoptosis and autophagy—Section 15.5 apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry
Scientific poster (2010) Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry  apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010) High content imaging of autophagy  antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader
Scientific poster (2010) Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis  Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010) A new generation of cellular imaging probes for neurosciences research  autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010) New fluorescent probes for live-cell imaging  autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis
Scientific poster (2011) Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011) Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats  Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2011) Validation of high content imaging and analysis assays for cell health and cytotoxicity  antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011) The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes  Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2014) New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy  autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader
Scientific poster (2015) Visualizing the life and death of cells: novel probes and platforms  apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
Webinar Basic techniques in autophagy research 
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)