Ion Indicators and Ion Channels Information

Learn about assays and techniques for detecting and monitoring ion channels and ion flux using fluorescent ion indicators by flow cytometry, imaging microscopy, high-content analysis and microplate assays. Topics include calcium and magnesium indicators, as well probes for potassium channel flux.

Ion indicators and ion channels features

BioProbes article

FluxOR II Green Potassium Ion Channel Assay—Next-generation detection of potassium ion flux

Potassium channels are ion-selective protein pores that span the cell’s plasma membrane and serve to establish and regulate membrane potential. In excitable cells such as neurons and myocytes, these channels function both to shape the action potential and to reset the cell’s resting membrane potential. The Invitrogen FluxOR II Green Potassium Ion Channel Assay is the newest tool for high-throughput detection of potassium ion channel and transporter activities.

Molecular Probes Handbook
Technical Note

Loading and Calibration of Intracellular Ion Indicators—Note 19.1

This article provides an overview of what you need to know to load ion indicators into living cells and how to calibrate your system to get the most out of the data.


Ion indicators and ion channels learning resources

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Type Title Categories
BioProbes article (2007) Fluorescent calcium indicators for in vivo imaging of neuronal networks  cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, in vivo imaging, ion indicators, neuroscience, two-photon microscopy
BioProbes article (2007) Listening in on cellular communication  cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, ion indicators, microplate reader
BioProbes article (2008) A no-wash indicator for high-throughput calcium detection  BacMam technology, cell signaling, drug discovery, flow cytometer/flow cytometry, fluorescent proteins, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2008) Potassium flux assay meets BacMam technology  BacMam technology, cell signaling, fluorescent dyes, genes, fluorescence microscopy/fluorescence imaging, ion flux/ion channels, ion indicators, microplate reader, reagents
BioProbes article (2008) Nuclear receptor antibodies: A core technology  antibodies, cell signaling, ELISA, gene expression, immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), ion flux/ion channels, western blotting
BioProbes article (2009) Innovative solutions for ion channel research  BacMam technology, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators, microplate reader
BioProbes article (2009) Neurons in focus: Two-photon excitation imaging in live neuronal tissue  Alexa Fluor, fluorescent dyes, fluorescent proteins, fluorescence microscopy/fluorescence imaging, ion flux/ion channels, ion indicators, live-tissue imaging, nanocrystals, neuroscience, Qdot, two-photon microscopy
BioProbes article (2009) Faster, easier calcium flux assays
cell signaling, fluorescent dyes,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, microplate reader
BioProbes article (2009) Assess hERG channel blocks without radioactivity
cardiovascular system, cell signaling, drug discovery, fluorescent dyes, ion flux/ion channels, microplate reader
BioProbes article (2009) High-throughput chloride channel screening
BacMam technology, fluorescent proteins,  fluorescence microscopy/fluorescence imaging, high content analysis, ion flux/ion channels, ion indicators
BioProbes article (2010) Choosing the right dye for imaging intracellular calcium calcium flux, fluorescence microscopy/fluorescence imaging, high content analysis, ion channels, ion flux, ion indicators, reagents, sample preparation
BioProbes article (2016) FluxOR II Green Potassium Ion Channel Assay—Next-generation detection of potassium ion flux high throughput screening, ion channels, ion flux, ion indicators
Molecular Probes Handbook MAXC Computer Program for calculating Free Ca2+ concentrations—Note 19.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Loading and calibration of intracellular ion indicators—Note 19.1 ion channels, ion flux, ion indicators
Molecular Probes Handbook Calcium regulation—Section 17.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Probes for ion channels and carriers—Section 16.3 ion channels, ion flux, ion indicators
Molecular Probes Handbook Probes useful at near-neutral pH—Section 20.2 BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes Handbook Probes useful at acidic pH—Section 20.3 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook pH indicator conjugates—Section 20.4 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Overview of pH indicators—Section 20.1 flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook Fluorescent Na+ and K+ indicators—Section 21.1 ion channels, ion flux, ion indicators
Molecular Probes Handbook Detecting chloride, phosphate, nitrite and other anions—Section 21.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Introduction to Ca2+ measurements with fluorescent indicators—Section 19.1 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Mg2+ indicators—Section 19.6 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent indicators for Zn2+ and other metal ions—Section 19.7 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Ca2+ indicators excited with visible light—Section 19.3 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Ca2+ indicators excited with UV light—Section 19.2 ion channels, ion flux, ion indicators
Molecular Probes Handbook Fluorescent Ca2+ indicator conjugates—Section 19.4 ion channels, ion flux, ion indicators
Molecular Probes Handbook Chelators, calibration buffers, ionophores and cell-loading reagents—Section 19.8 ion channels, ion flux, ion indicators
Molecular Probes Handbook Protein-based Ca2+ indicators—Section 19.5 calcium flux, fluorescent dyes, ion indicators
Scientific poster (2008) Living molecular probes for the next generation  BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging
Scientific poster (2008) Cell-based potassium ion channel screening with the FluxOR Assay  BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008) Improving downstream results with automated cell counting  automated cell counter, BacMam technology, cell counting, cell expansion, cell isolation, Countess, fluorescent dyes, hemocytometer, ion flux/ion channels, microplate reader
Scientific poster (2008) FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets  BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2009) Cell-based potassium ion channel screening with BacMam using the FluxOR Assay  BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2010) A new generation of cellular imaging probes for neurosciences research  autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010) BacMam gene expression for functional and imaging applications  BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
Video LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System 
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging