Support for your Tali instrument

The Invitrogen™ Tali™ Image-based Cytometer has been discontinued, but you can still find consumables and accessories, software download information, and compatible reagents, as well as videos and application notes for your instrument (see below).

The Countess™ II FL Automated Cell Counter is a fully automated, 3-channel cell counter to rapidly analyze fluorescently labeled cells or trypan blue stained cells in suspension.

  • Two, user-defined fluorescent channels to fit your research application
  • Optional reusable slide to reduce lab consumable costs
  • Capture cell counts in under 10 seconds

Tali Image-based Cytometer resources

Product manual

Application notes

Citations


Tali Demo
 

Apoptosis with Tali
 

Fluorescence with Tali
 

Cell Cycle with Tali

The Tali Image-Based Cytometer can be used to detect green and red fluorescence of a variety of dyes and stains. This table ranks the fluorescence intensity of various dyes when examined on the Tali Image-Based Cytometer. Unless otherwise indicated, cells were labeled with the indicated fluorescent dye or stain at concentrations suggested in the product manual and analyzed on the Tali Image-Based Cytometer using the “Green+Red Setting” setting. In some cases, there was spectral overlap of the fluorescence emission (commonly known as bleedthrough) into the opposite channel. This information is also included in the table.

Red channel dyes

Dye, product tested Excitation (nm) Emission (nm) Expected emission channel Green channel signal Red channel signal Recommendations
7-AAD (7-aminoactinomycin D) 549 645 Red
None
+++
Use 1–2 uL per mL
R-PE (R-phycoerythrin), CD3-RPE conjugate 564 577 Red
None
++
Test titration of antibody conjugate
CellROX™ Orange Reagent 545 565 Red None
+++
Use according to product manual
MitoTracker™ Orange CM-H2TMRos 551 575 Red
None
++
Use according to product manual
Ethidium homodimer-1 528 618 Red
None
+++
Use 1 uM
Rhodamine Red-X anti-mouse secondary 573 591 Red
None
+
Basically undetectable

 

Green channel dyes

Dye, product tested Excitation (nm) Emission (nm) Expected emission channel Green channel signal Red channel signal Recommendations
SYTOX™ Green stain 504 523 Green
+++
+
Use 12.5 nM
LIVE/DEAD™ Fixable Green Dead Cell Stain 495 520 Green
+++
++
Spectral overlap into red channel at lowest concentration tested (0.25 µL/mL)
CellTrace™ Green 492 517 Green
+++
+
Use at 50 nM–250 nM
CellEvent™ Green reagent 502 530 Green
++
None
10 µM final concentration
SYTO™ 9 stain 485 498/501 Green
+++
None
Use 100 nM
TO-PRO™-1
515 531 Green
++
++
Due to spectral overlap can not be used for multiplexing experiments
FM™ 1-43X 510 626 Green
+++
+++
Due to spectral overlap can not be used for multiplexing experiments
CellTracker™ Green CFDA-AM 492 517 Green
+++
None
Use according to product manual
5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate (carboxy-DCFDA), mixed isomers 505 525 Green
++
None
Use at 25–50 uM
Annexin V-FITC 494 518 Green
++
None Use according to product manual (test 10 uL Annexin V-FITC if signal is dim)
Acridine Orange 500 526 Green
+++
None For Jurkat cells suggest using 0.25 – 0.5 uM (test titration of the dye to ensure no overlap into the red channel)
Calcein AM 494 517 Green
+++
+
Use at 0.1–1 uM (test titration of dye to ensure no overlap into red channel).

+ = Undetectable to barely detectable on the histogram, but can see spots on the Tali images.
++ = Can detect peak on histogram, but may be difficult to separate positive and negative peaks.
+++ =  Easily detectable peaks on histogram. Positive and negative populations can be easily resolved.

Before you install

Register your instrument

As a precaution when installing any update, save your vital data from the Tali Image-Based Cytometer:

  1. Insert a USB drive into the Tali Image-Based Cytometer
  2. Press the ‘Data’ tab at the top of the screen
  3. Select the files you wish to save to the USB drive
  4. Select Export format; choose either Data tables (.csv), Images (.jpg), Report (.pdf) or All formats
  5. When the file(s) transfer is complete, remove the USB drive and transfer files to your computer

Note: If you experience system instability when exporting multiple files, all data are preserved.  Turn the instrument off and then on to reinitialize the system.

Step 1—Download and save to a clean USB drive:

Download the current software v2.1 (release date 10/30/2012) and save it to a clean USB drive.

Download Tali software v2.1 (4.3 MB)

NOTE: Please contact Technical Support if you are updating from software version 1.0.6.5.

a. Press the download button and save the Tali Software zip file to your computer.
b.
Obtain a USB Drive and remove all files or programs from this drive. Existing files can interfere with the software installation process thus it is important to remove all files or programs from the USB drive before continuing.
c.
Using the clean USB drive, place the three programs and the image file from the zip file onto the USB drive.
d.
After the transfer, review the file and program configuration of the USB Drive. The configuration should be the following (see below).

Step 2—Transfer files to your Tali® Image Cytometer

a. Insert the USB drive into the USB port located on the front of the Tali Instrument.
b. Press the ‘Settings’ tab (highlighted with the red box below).

c. Press the 'Update firmware' button (highlighted with the red box below).

d. Wait until the Tali instrument recognizes the USB drive and the green light is visible on the 'Update' button (see below).

e. Press the 'Update' button.
f. When the Update is complete the instrument will need to be restarted. Press the ‘OK’ button (see below).

g. After the instrument performs the restart, press the ‘Settings’ tab and check that the software has been updated to the latest version (see below).