Great expectations for in vivo vasculature imaging
Vascular contrast reagents to fit your experimental needs
Conjugates to measure capillary leakage
SAIVI™ Alexa Fluor® 680 and Alexa Fluor® 750 Injectable Contrast Agents Serum proteins albumin and transferrin are common tumor markers that also play a role of transport of cytostatic agents into tumors. By conjugating BSA and transferrin to Molecular Probes™ near infrared (NIR) dyes, these are an effective tool for cancer research.
These contrast agents will accumulate in tumors, making visualization of tumors in small animals easy. Within 60 minutes of injection, these agents will clearly delineate the blood vessels in tumors. Over time the BSA and transferrin conjugates will readily leak from the capillaries and diffuse into the intersitital space, and thus more generally highlight the tumor. This can be particularly useful in studies to look at the effects of a cancer drug on the vasculature or to compare normal and the more highly leaky tumor vasculature.
In most cases the BSA contrast agent is recommended due to the speed of the labeling. However, if using a human product is important to you transferrin is a good alternative agent. Also, some tumors may have a high level of transferrin receptors and this will enhance the labeling, and thus the transferrin reagent can be used as a tumor marker in transferrin positive cancers.
SAIVI™ In Action
View a short video that demonstrates the vascular labeling and capillary leakage in a human tumor xenograft in a living mouse with the SAIVI™ Alexa Fluor® 750 injectable Contrast Agent *bovine serum albumin* as imaged using the CRi Maestro™ imaging system.
Distinguish vasculature using non-targeted Qdot® nanocrystals
| Ideal for vascular imaging, the Qtracker® non-targeted quantum dots provide a novel in vivo solution for viewing normal vasculature down to the capillaries. In contrast to the BSA and transferrin conjugated vasculature agents, the Qdot® nanocrystals have minimal leakage from the vasculature, and allow imaging for at least 3 hours.
The Qtracker® non-targeted quantum dots will specifically label just the vessels, and are retained within the vessels, as shown in Figure 1.
Figure 1. Qtracker® non-targeted quantum dots 800 labeling mouse vasculature in vivo. Image was obtained using the CRi Maestro™ imaging system.
Get more information by multiplexing
Figure 2. Multiplex imaging of tumor cells and vasculature
Tumor cells are labeled with Anti-CEA Ab conjugated to Alexa Fluor® 680. The vasculature is labeled with Qtracker® 655 non-targeted quantum dots. Images were obtained using the CRi Maestro™ imaging system.