Efficient. Flexible. Transformative.

The Attune NxT system makes multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow. Designed for faster experimental run times, this compact flow cytometer offers proven, rapid and accurate analysis for a broad range of sample types.

  • Efficient—up to 10X faster than traditional flow cytometric analyzers
  • Flexible—up to 14 colors; field upgradeable
  • Transformative—run difficult samples with a system that is less sensitive to clogging

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A flow cytometer designed with your research in mind

  • Conserve sample and quickly run experiments with sample flow rates ranging from 12.5–1,000 μL/min
  • Get up to speed quickly with an intuitive software interface and easy-to-follow workflow that is quick to learn, teach and use
  • Save time and minimize cell handling with faster protocols

Freedom of choice

  • Configurable laser and filters provide more choices when designing multicolor panels
  • Broaden your capabilities with field upgradeability of up to 4 lasers and 14 colors
  • Expand multiparameter capabilities with detection of dim to very bright signals
  • See compatible reagents by instrument configuration or detection channel

An evolutionary flow cytometer

Automation for any lab

Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.

  • Autosampler is compact and compatible with many different types of plates
  • No carousel needed; run deep well plates up to 2 mL
  • Rapid processing of up to 384 samples

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Attune NxT Flow Cytometer configuration options

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
Available as upgrade†
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
Available as upgrade†
Available as upgrade† 9
Available as upgrade†
  Available as upgrade† 9
Available as upgrade† Available as upgrade† Available as upgrade† 10
Available as upgrade†
Available as upgrade† Available as upgrade†
3 Blue/green/red
Available as upgrade†
Available as upgrade† 13
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/violet/yellow (A24859)
Available as upgrade† 13
Available as upgrade† Available as upgrade†
4 Blue/red/violet/green
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.
  • Laser power (as shown in table below)
    Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
    Violet 405 50 100
    Blue 488 50 100
    Green 532 100 140
    Yellow 561 50 100
    Red 637 100 140
    * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
    ** Vendor-specified theoretical maximum.
  • Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection 
  • Laser profile: 10 μm x 50 μm flat-top laser providing robust alignment 
  • Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters 
  • Laser separation: 150 μm 
  • Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
  • Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off 
  • Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage
  • Flat top specified at the flow cell: CV <3% over width of flat top
  • Upgradable: Convenient field changes
  • Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
  • Sample analysis volume: 20 μL to 4 mL
  • Custom sample flow rates: 12.5–1,000 μL/min
  • Sample delivery: Positive-displacement syringe pump for volumetric analysis
  • Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
  • Fluid-level sensing: Active
  • Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
  • Fluid storage: All fluids stored within instrument
  • Extended fluidics option: Configuration for 10 L fluid
  • Nominal fluid consumption: 1.8 L/day
  • Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
  • Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
  • Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
  • Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
  • Maximum electronic speed: 65,000 events/sec with all parameters
  • Carryover: Single-tube format: <1%
  • Forward and side scatter sensitivity: Able to discriminate platelets from noise
  • Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
  • Forward scatter: Photodiode detector with 488/10 nm bandpass filter
  • Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
  • Fluorescent detectors: 14 individual detectors
  • Electronic pulse: Measured area, height and width pulse for all detectors
  • Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
  • Minimum particle size: 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories
  • Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
  • Flow rate: Precise flow rate control via software; no hardware adjustments
  • Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
  • Overlays: Comparative analysis between samples; 3D view
  • Sample recovery: System able to return unused samples
  • Concentration: Direct concentration measurement without use of counting beads
  • Software layout: Fully customizable for each user account
  • Bubble detection technology: Stops automated run to preserve sample integrity
  • Maximum single-event file: 20 million with option to append
  • Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
  • Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
  • Gating: Hierarchal gating with the ability to derive gates
  • Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
  • Voltage: User adjustable
  • Window extensions: User adjustable
  • Area scaling factor (ASF): User adjustable
  • Acquisition settings: Documented in FCS files and maintained upon import
  • Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
  • Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
  • Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
  • User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count
Quality and regulatory
  • Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
  • Warranty: 1 year
  • Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
  • Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
  • Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
  • For Research Use Only
Data management
  • Software requirements: Invitrogen Attune NxT Software
  • Monitor: 23-inch flat panel (1,920 x 1,200 resolution); dual-monitor capability
  • Computer: Minitower desktop
  • Operating system: Windows™ 7 64-bit
  • FCS format: FCS 3.1, 3.0
  • Processor: Intel Core™ i7 processor
  • RAM: 16 GB
  • Hard drives: 80 GB or larger and 250 GB RAID-compatible hard drives
Installation requirements
  • Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
  • Heat dissipation: <150 W
  • Temperature operating ranges: 15–30°C (59–86°F)
  • Operating humidity: 10–90%, noncondensing
  • Audible noise: <65 dBA at 1.0 m
  • Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
  • Weight: ~29 kg (64 lb)
  • Available configurations (see Attune NxT Flow Cytometer configuration options table)

Simple yet powerful software, easy auto compensation

Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:

  • Compensation—fully automated and manual compensation
  • Instrument tracking—automated baseline and performance test for all channels and linearity
  • Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
    monthly decontamination
  • User account maintenance—administrative and individual accounts with user log
  • Gates—standard and customizable gates
  • Plot previews—easy workspace setup
  • Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
  • File formats—FCS 3.0 and FCS 3.1
  • Graphics resolution—publication-quality images (customizable)

Learn more about software for the Attune NxT Flow Cytometer

  • Software for the Attune NxT Flow Cytometer ›
  • Literature


    Application notes

    Service plans

    Optimize your instrument performance, maximize the availability of your critical systems, and streamline your laboratory workflows. Choose the level of support for your Attune NxT Flow Cytometer that best meets your laboratory's needs and budget:

    • AB Complete Plan
    • AB Assurance Plan
    • AB Maintenance PLUS Plan

    Consumables and accessories

    In addition to accessories such as sample syringes, find the Invitrogen Attune solutions necessary to operate your Attune NxT Flow Cytometer including:

    • Attune Focusing Fluid
    • Attune Wash Solution
    • Attune Shutdown Solution
    • Attune Performance Tracking Beads

    Flow Panel Design Tool

    Choose antibody conjugates for your panel in 3 steps: 1) pick the antibody species reactivity, 2) select up to 14 targets, and 3) choose the lasers or fluorophores you want to view.

    Fluorescence SpectraViewer

    This online tool allows researchers to assess the spectral compatibility of dyes and probes in the course of designing experiments that utilize fluorescence detection techniques.