Powerful, flexible flow cytometer

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Look beyond the status flow

Run samples faster and achieve greater resolution—with little fear of sample loss due to clogging.

The Invitrogen Attune NxT Flow Cytometer combines precision with performance in a true benchtop flow cytometer with up to 4 lasers and 16 parameters of detection. The Attune NxT Flow Cytometer was developed with the goal of removing barriers associated with flow cytometry and enabling new science. This includes bringing the power of flow cytometry to the analysis of more sample types, including cancer cells. The addition of the  Invitrogen Attune Autosampler helps enable significantly faster high-throughput multiparametric screening.

Save time—10x faster with no loss in data quality

What you currently spend all day doing could be done in a fraction of that time. Let the Attune NxT Flow Cytometer enable YOUR science—a faster acquisition rate not only speeds up the completion of your experiment, it puts true discovery within reach. So don’t leave those samples languishing in the freezer; analyze them today.

  • True 35,000 events/sec acquisition and 1 mL/min flow rates without losing data, at acquisition transmission speed equivalent to streaming 10 HD movies
  • Up to 10x faster than traditional cytometers, thanks to acoustic focusing technology, elegant fluidic design, optimized optics, and enhanced data transmission
  • Run your samples in a fraction of the time needed for most instruments
  • Dilute your difficult samples and get the additional data your research needs
  • Find the needle in the haystack; find a new cell population (this researcher did)
  • Use sample types you could not before

Attune NxT achieves superior sample throughput

The Attune NxT Flow Cytometer, designed using acoustic-assisted hydrodynamic focusing, achieves sample-throughput rates of of 12.5 µL/min–1 mL/min or 12.5–1,000 µL/min—up to 10 times faster than traditional hydrodynamic focusing systems and data acquisition speeds of 35,000 events/sec.

Attune NxT Flow Cytometer acquires 1,000,000 events

The Attune NxT Flow Cytometer acquires 1,000,000 events in a fraction of the time required compared to three competitor instruments.  Data represents protocol time achieved by running the flow cytometers at maximum sample rates for each instrument (1,000 µL/min for the Attune NxT Flow Cytometer).

See more data

Attune consistency table

Minimal data variation at high sample rates with the Attune NxT Flow Cytometer. Jurkat cells were fixed and stained with propidium iodide, treated with RNase and analyzed at a concentration of 1 x 106 cells/mL on the Attune NxT Flow Cytometer. Regardless of sample rate, the coefficient of variation (CV) of cells in the G0/G1 and G2/M phases remain consistent, even at the highest sample rate of 1,000 μL/min.

Modular design, up to 4 lasers and 14 colors

The Invitrogen Attune NxT Flow Cytometer can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry to match the panels you are currently running. Multiple fluorescent proteins can be detected with an optional 561 nm laser. Whether you configure your system now or upgrade later, the Attune NxT flow cytometer can grow with you and your research needs.

Attune NxT Flow Cytometer configuration options

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
Available as upgrade†
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
Available as upgrade†
Available as upgrade† 9
Available as upgrade†
  Available as upgrade† 9
Available as upgrade† Available as upgrade† Available as upgrade† 10
Available as upgrade†
Available as upgrade† Available as upgrade†
3 Blue/green/red
Available as upgrade†
Available as upgrade† 13
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/violet/yellow (A24859)
Available as upgrade† 13
Available as upgrade† Available as upgrade†
4 Blue/red/violet/green
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

Clogging has met its match

The Attune NxT Flow Cytometer with clog-resistant engineering brings the power of flow cytometry to more sample types, including tumor samples, cardiomyocytes, and various adherent cells types. 

  • Minimize the hassle, frustration, and sample loss associated with a clogged instrument
  • Investigate difficult samples and large, clumpy cells, including cancer cells, cardiomyocytes, and adipocytes, which were previously not amenable to flow cytometry
Flow cytometers traditionally have not performed well with large clumpy samples, as they often resulted in system clogging leading to sample loss, project delays, and need for significant monitoring of instrument runs. The Attune NxT cytometer easily handles larger, clumpy cell samples that other flow cytometers cannot. These include tumor samples, cardiomyocytes, adipocytes, and many other types that were previous excluded, bringing high-throughput statistical analysis to an increasing number of sample types. 

Expand your capabilities as your research needs expand

Simplified sample prep—no wash, no lyse options

Acoustic focusing allows significantly higher sample rates, which simplifies sample preparation, reduces protocol times, and minimizes cell loss. This feature is useful for no wash, no lyse immunophenotyping protocols using whole blood and for samples that are inherently low in concentration, including cerebrospinal fluid and stem cell preparations.

Acoustic focusing allows significantly higher sample rates

Attune NxT Flow Cytometer is able to perform immunophenotyping analysis on minimally processed samples, reducing your 10-step sample prep protocol to 3 easy steps.

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Walk-away automation and simple, fast plate/tube conversion

The Attune NxT Flow Cytometer with Autosampler brings the power of flow cytometry’s multiparametric screening to a significantly increased number of sample types and cell lines in a high-throughput manner.

  • Flexible—Read 96- and 384-well plates, including deep well plates up to 2 mL
  • Simple—Easily convert between plates and tubes in seconds with the click of a mouse. No hardware changes or shutdown/restarts required, in contrast to the 30–45 min off-line time needed by other systems
  • Freeing—Designed for walk-away performance with clog-resistant fluidics and software

Enables unique applications

Your next result relies on the ability of your experimental design and your instrument to deliver more—more data, more detail, and more throughput. The Attune NxT Flow Cytometer was designed to meet the demands of more complex experiments, offering reliable data, day after day, in a grow-with-you system.

See the data

  • Software for the Attune NxT Flow Cytometer ›
  • Optics
    • Laser power (as shown in table below)
      Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
      Violet 405 50 100
      Blue  488 50 100
      Green  532 100 140
      Yellow 561 50 100
      Red 637 100 140
      * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
      ** Vendor-specified theoretical maximum.
    • Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection 
    • Laser profile: 10 μm x 50 μm flat-top laser providing robust alignment 
    • Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters 
    • Laser separation: 150 μm 
    • Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance require
    • Onboard thermal-electric cooler: No warm-up delay; fiber isn't affected by on/off 
    • Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it "on" when acquiring samples; reports hours of usage 
             ––Flat top specified at the flow cell; CV <3% over width of flat top
             ––Field upgradable
    • Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
    • Sample analysis volume: 20 μL to 4 mL
    • Custom sample flow rates: 12.5–1,000 μL/min
    • Sample delivery: Positive-displacement syringe pump for volumetric analysis
    • Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
    • Fluid-level sensing: Active
    • Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
    • Fluid storage: All fluids stored within instrument
    • Extended fluidics option: Configuration for 10 L fluid
    • Nominal fluid consumption: 1.8 L/day
    • Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
    • Fluorescence sensitivity: ≤80 MESF for FITC, ≤30 MESF for PE, ≤70 MESF for APC
    • Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
    • Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
    • Maximum electronic speed: 65,000 events/sec with all parameters
    • Carryover: Single-tube format: <1%
    • Forward and side scatter sensitivity: Able to discriminate platelets from noise
    • Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
    • Forward scatter: Photodiode detector with 488/10 nm bandpass filter
    • Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
    • Fluorescent detectors: 14 individual detectors
    • Electronic pulse: Measured area, height and width pulse for all detectors
    • Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
    • Minimum particle size: 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories
    • Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells
    • Flow rate: Precise flow rate control via software; no hardware adjustments
    • Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec
    • Overlays: Comparative analysis between samples; 3D view
    • Sample recovery: System able to return unused samples
    • Concentration: Direct concentration measurement without use of counting beads
    • Software layout: Fully customizable for each user account
    • Bubble detection technology: Stops automated run to preserve sample integrity
    • Maximum single-event file: 20 million with option to append
    • Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates
    • Threshold: Up to 4 individual thresholds with user option to apply Boolean logic
    • Gating: Hierarchal gating with the ability to derive gates
    • Smartgate labeling: Option to annotate quad gate names based on fluorophore and target names
    • Voltage: User adjustable
    • Window extensions: User adjustable
    • Area scaling factor (ASF): User adjustable
    • Acquisition settings: Documented in FCS files and maintained upon import
    • Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously
    • Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
    • Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software)
    • User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count
    Quality and regulatory
    • Instrument tracking: Automated daily baseline and performance test with Levey-Jennings plots
    • Warranty: 1 year
    • Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications
    • Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003
    • Robust install specifications: Units installed by engineer; preplanning checklist, delivery, installation, and performance validation comply with standardized procedure
    • For Research Use Only
    Data management
    • Software requirements: Invitrogen Attune NxT Software
    • Monitor: 23-inch flat panel (1,920 x 1,200 resolution); dual-monitor capability
    • Computer: Minitower desktop
    • Operating system: Windows™ 7 64-bit
    • FCS format: FCS 3.1, 3.0
    • Processor: Intel Core™ i7 processor
    • RAM: 16 GB
    • Hard drives: 80 GB or larger and 250 GB RAID-compatible hard drives
    Installation requirements
    • Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune NxT Flow Cytometer conforms to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune NxT Flow Cytometer is a Class I laser product per CDRH regulations and EN/IEC 60825.
    • Heat dissipation: <150 W
    • Temperature operating ranges: 15–30°C (59–86°F)
    • Operating humidity: 10–90%, noncondensing
    • Audible noise: <65 dBA at 1.0 m
    • Instrument size (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles
    • Weight: ~29 kg (64 lb)
    • Available configurations (see Attune NxT Flow Cytometer configuration options table)

    Simple yet powerful software, easy auto compensation

    Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:

    • Compensation—fully automated and manual compensation
    • Instrument tracking—automated baseline and performance test for all channels and linearity
    • Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
      monthly decontamination
    • User account maintenance—administrative and individual accounts with user log
    • Gates—standard and customizable gates
    • Plot previews—easy workspace setup
    • Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
    • File formats—FCS 3.0 and FCS 3.1
    • Graphics resolution—publication-quality images (customizable)

    Learn more about software for the Attune NxT Flow Cytometer

    Scientific posters

    High Sensitivity Applications for the Attune Acoustic Focusing Cytometer
    Presented by Bill Telford, head of the Experimental Transplantation and Immunology Branch's FACS Core Facility at CYTO 2012.
    Studying Malaria using the Attune Acoustic Focusing Cytometer
    Presented by Grace Chojnowski, Flow Cytometry and Imaging Facility manager at Queensland Institute of Medical Research at CYTO 2012.
    A Novel No-Wash, No-Lyse Method For Residual Leukocyte Enumeration Using Acoustic Focusing Flow Cytometry and a Cell-Membrane Permeant DNA Dye
    Presented by José-Enrique O'Connor, head of the Laboratory of Cytomics at Centro de Investigación Príncipe Felipe at CYTO 2012.
    Ultrasmall And Beyond: Nuclear Holopoloid Genome Sizing of Carnivorous Plant Species by Acoustic Focusing Cytometry
    Flow cytometry has been widely utilized as an expeditious and accurate method for the estimation of plant holoploid nuclear genome size (C‐value).
    Phospho‐Protein Detection Using Acoustic Cytometry
    Multiparameter flow cytometry is an important tool for examining cell signaling pathways. Using this acoustic focusing technology the sample stream can be slowed down...
    Acoustic Focusing Cytometry: Sensitivity and Throughput
    We examined changes in sensitivity with sample flow rate using acoustic focusing cytometry. We performed typical assays using the Applied Biosystems Attune Acoustic Focusing Cytometer...
    Acoustic Flow Cytometry for Multicolor Immunophenotyping Analysis
    Acoustic focusing enables both longer cell interrogation times and higher throughput, which provide higher-precision analysis of dilute samples without compromising the speed of collection...
    Identification and Initial Characterization of Limbal Stem Cells Isolated From Human Cornea
    Limbal stem cells are known to be precursor cells that repopulate the cornea during wound healing and corneal repair. Limbal stem cells in the basal region give rise to transit amplifying (TA) cells...
    Rare Event Detection using Acoustic Cytometry
    Detection of rare events (defined as populations of cells that make up less than 5% of the total cells), includes the detection of stem cells, minimal residual disease, tetramers, NKT cells, and fetomaternal...
    Acoustic Cytometry for Rare Event Detection of PNH Cells
    Flow cytometry is the method of choice for detection of paroxysmal nocturnal haemoglobinuria (PNH), and is made possible with technological improvements in instrumentation combined with...

    Service Plans

    Optimize your instrument performance, maximize the availability of your critical systems, and streamline your laboratory workflows. Choose the level of support for your Attune NxT Flow Cytometer that best meets your laboratory's needs and budget.

    • AB Complete Plan
    • AB Assurance Plan
    • AB Maintenance PLUS Plan

    Consumables and Accessories

    In addition to accessories such as sample syringes, find the Invitrogen Attune solutions necessary to operate your Attune NxT Flow Cytometer including:

    • Attune Focusing Fluid
    • Attune Wash Solution
    • Attune Shutdown Solution
    • Attune Performance Tracking Beads