photo of the Attune NxT Flow Cytometer

Powerful, flexible flow cytometer

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Modular design, up to 4 lasers and 14 colors

The Invitrogen™ Attune™ NxT Flow Cytometer can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry to match the panels you are currently running. Multiple fluorescent proteins can be detected with an optional 561 nm laser. Whether you configure your system now or upgrade later, the Attune NxT flow cytometer can grow with you and your research needs.

Attune NxT Flow Cytometer configuration options

Lasers Laser configuration
(Cat. No.)
Lasers included Detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Red, 637 nm
1 Blue
Available as upgrade†
Available as upgrade† Available as upgrade† 6
2 Blue/yellow
Available as upgrade†
Available as upgrade† 9
Available as upgrade† Available as upgrade† 10
Available as upgrade†
Available as upgrade†
3 Blue/violet/yellow (A24859)
Available as upgrade† 13
Available as upgrade†
Blue/red/yellow (A28993) Available as upgrade† 12
4 Blue/red/yellow/violet
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, or Red Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

Save time—10X faster speed with no loss in data quality

The Attune NxT Flow Cytometer, designed using acoustic-assisted hydrodynamic focusing, achieves sample-throughput rates of of 12.5 µL/min–1 mL/min or 12.5–1,000 µL/min—up to 10 times faster than traditional hydrodynamic focusing systems and data acquisition speeds of 35,000 events/sec.

bar graph comparing speed of Attune NxT Cytometer to competitor instruments

The Attune NxT Flow Cytometer acquires 1,000,000 events in a fraction of the time required compared to three competitor instruments.  Data represents protocol time achieved by running the flow cytometers at maximum sample rates for each instrument (1,000 µL/min for the Attune NxT Flow Cytometer).

See more data ›


table showing similar CV values at six different sample rates

Minimal data variation at high sample rates with the Attune NxT Flow Cytometer. Jurkat cells were fixed and stained with propidium iodide, treated with RNase and analyzed at a concentration of 1 x 106 cells/mL on the Attune NxT Flow Cytometer. Regardless of sample rate, the coefficient of variation (CV) of cells in the G0/G1 and G2/M phases remain consistent, even at the highest sample rate of 1,000 μL/min.

Simplified sample prep—no wash, no lyse options

Acoustic focusing allows significantly higher sample rates, which simplifies sample preparation, reduces protocol times, and minimizes cell loss. This feature is useful for no wash, no lyse immunophenotyping protocols using whole blood and for samples that are inherently low in concentration, including cerebrospinal fluid and stem cell preparations.

flow chart comparison showing 3 step immunophenotying via Attune flow cytometer compared to 10 steps using a traditional hydrodynamic focusing cytometer

Attune NxT Flow Cytometer is able to perform immunophenotyping analysis on minimally processed samples, reducing your 10-step sample prep protocol to 3 easy steps.

See more data ›

Enables unique applications

Your next result relies on the ability of your experimental design and your instrument to deliver more—more data, more detail, and more throughput. The Attune NxT Flow Cytometer was designed to meet the demands of more complex experiments, offering reliable data, day after day, in a grow-with-you system.

See the data ›

  image of a scatter plot generated from the Attune NxT flow cytometer

Attune NxT Flow Cytometer instrument specifications

Physical characteristics Footprint (H x W x D): Approximately 40 cm (16 in) × 58 cm (23 in) × 43 cm (17 in)
Weight: Approximately 29 kg (64 lb)
Operating temperature: 15–30°C
Operating humidity: 10–90%, noncondensing
Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W
Audible noise: <65 dBA at 1.0 m
Optics The optical layout is dependent upon the instrument configuration chosen from the 1–4 laser system.

Laser power:
◼ Blue laser: 488 nm, 50 mW
◼ Violet laser: 405 nm, 50 mW
◼ Red laser: 637 nm, 100 mW
◼ Yellow laser: 561 nm, 50 mW

Laser profile: Flat top laser requiring minimal alignment
Flow cell: Quartz cuvette gel coupled to 1.2 NA collection lens
Alignment: Fixed alignment, no customer maintenance required
Emission Forward scatter: Photodiode detector with 488/10 bandpass filter
Side scatter: PMT with 488/10 bandpass filter
Emission filters: User-changeable, keyed filters
Up to 14 color channels with PMTs
Fluidics Sample rates: 12.5–1,000 μL/min
Sample delivery: Sample delivered by positive displacement syringe pump for volumetric analysis
Sample analysis volume: 20 μL–4 mL
Fluid storage: All fluids stored within instrument with active fluid level sensing
Standard fluidic tanks: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
External tanks option: Optional configuration for 10 L fluid
Nominal fluid consumption: 1.8 L/day
Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
Work station Operating system: Windows® 7 SP1
Processor: Intel® Core™ i7
RAM: 16 GB
Computer: Minitower desktop
Hard drive: 80 GB or larger and 250 GB RAID-compatible hard drives
Monitor: 23-inch flat panel (1920 x 1200 resolution), dual monitor capability
Software ◼ Attune NxT Software
◼ Romlock license required
Software features Compensation: Fully automated and manual compensation modes
Instrument tracking: Automated baseline and performance test with Levey-Jennings plots
Automated maintenance: ≤15 min startup and shutdown
Maximum event file: 20 million
Heat map: Tubes and plate visualization
SmartGate™ label: For Quad
Stats: Create customized statistics (i.e., Concentration)
File formats: FCS 3.1 (saved)
Graphics resolution: Publication-quality images
User account maintenance: Administrative and individual accounts with user log
Gates: Standard and customizable gates
Thermo Fisher Cloud Save files to Cloud through computer
Performance Data acquisition rate: Up to 35,000 events/sec
Particle size range: 0.5–50 μm
Fluorescence sensitivity:

◼ ≤ 80 MESF FITC
◼ ≤ 30 MESF PE
◼ ≤ 70 MESF APC
Fluorescence resolution CV <3% for the singlet peak of propidium iodide–stained CEN
Forward and side scatter ◼ Able to discriminate platelets from noise
◼ Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood

Simple yet powerful software, easy auto compensation

Invitrogen™ Attune™ NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:

  • Compensation—fully automated and manual compensation
  • Instrument tracking—automated baseline and performance test for all channels and linearity
  • Automated maintenance—startup (<15 min), shutdown, rinse, wash, debubble, deep clean,
    monthly decontamination
  • User account maintenance—administrative and individual accounts with user log
  • Gates—standard and customizable gates
  • Plot previews—easy workspace setup
  • Zoom filmstrip viewer—easy visualization and navigation of plots to set gates and plot attributes
  • File formats—FCS 3.0 and FCS 3.1
  • Graphics resolution—publication-quality images (customizable)

Learn more about software for the Attune NxT Flow Cytometer

Walkaway automation

An optional accessory to the Attune NxT Flow Cytometer, the Invitrogen™ Attune™ NxT Autosampler, enables rapid processing of up to 384 samples.

Learn more about the Attune NxT Autosampler

Scientific posters

High Sensitivity Applications for the Attune Acoustic Focusing Cytometer
Presented by Bill Telford, head of the Experimental Transplantation and Immunonology Branch's FACS Core Facility at CYTO 2012.
Studying Malaria using the Attune® Acoustic Focusing Cytometer
Presented by Grace Chojnowski, Flow Cytometry and Imaging Facility manager at Queensland Institute of Medical Research at CYTO 2012.
A Novel No-Wash, No-Lyse Method For Residual Leukocyte Enumeration Using Acoustic Focusing Flow Cytometry and a Cell-Membrane Permeant DNA Dye
Presented by José-Enrique O'Connor, head of the Laboratory of Cytomics at Centro de Investigación Príncipe Felipe at CYTO 2012.
Ultrasmall And Beyond: Nuclear Holopoloid Genome Sizing of Carnivorous Plant Species by Acoustic Focusing Cytometry
Flow cytometry has been widely utilized as an expeditious and accurate method for the estimation of plant holoploid nuclear genome size (C‐value).
Phospho‐Protein Detection Using Acoustic Cytometry
Multiparameter flow cytometry is an important tool for examining cell signaling pathways. Using this acoustic focusing technology the sample stream can be slowed down...
Acoustic Focusing Cytometry: Sensitivity and Throughput
We examined changes in sensitivity with sample flow rate using acoustic focusing cytometry. We performed typical assays using the Applied Biosystems® Attune® Acoustic Focusing Cytometer...
Acoustic Flow Cytometry for Multicolor Immunophenotyping Analysis
Acoustic focusing enables both longer cell interrogation times and higher throughput, which provide higher-precision analysis of dilute samples without compromising the speed of collection...
Identification and Initial Characterization of Limbal Stem Cells Isolated From Human Cornea
Limbal stem cells are known to be precursor cells that repopulate the cornea during wound healing and corneal repair. Limbal stem cells in the basal region give rise to transit amplifying (TA) cells...
Rare Event Detection using Acoustic Cytometry
Detection of rare events (defined as populations of cells that make up less than 5% of the total cells), includes the detection of stem cells, minimal residual disease, tetramers, NKT cells, and fetomaternal...
Acoustic Cytometry for Rare Event Detection of PNH Cells
Flow cytometry is the method of choice for detection of paroxysmal nocturnal haemoglobinuria (PNH), and is made possible with technological improvements in instrumentation combined with...

Service Plans

Optimize your instrument performance, maximize the availability of your critical systems, and streamline your laboratory workflows. Choose the level of support for your Attune NxT Flow Cytometer that best meets your laboratory's needs and budget.

  • AB Complete Plan
  • AB Assurance Plan
  • AB Maintenance PLUS Plan

Consumables and Accessories

In addition to accessories such as sample syringes, find the Invitrogen™ Attune™ solutions necessary to operate your Attune NxT Flow Cytometer including:

  • Attune Focusing Fluid
  • Attune Wash Solution
  • Attune Shutdown Solution
  • Attune Performance Tracking Beads