2018 Attune NxT Flow Cytometer Violet 6-Channel Option
Expand your violet laser capabilities
The 2018 Invitrogen Attune NxT Flow Cytometer with acoustic focusing technology is now available with 6 fluorescence detectors off the violet laser, allowing measurement of up to 11 fluorescence parameters with a 2-laser instrument, 12 fluorescence parameters with a 3-laser instrument, or 14 fluorescence parameters with a 4-laser instrument (Table 1).
- 6 fluorescence channels, can accommodate the Super Bright antibody conjugates
- Detect more data from a single sample and streamline panel design
- Help save time and sample with less clogging
Table 1. Attune NxT Flow Cytometer configuration using six fluorescence detectors for the violet laser.
| Laser | Fluorescence detectors | ||
|---|---|---|---|
| 2-laser | 3-laser | 4-laser | |
| Violet, 405 nm | 6 | 6 | 6 |
| Blue, 488 nm | 3 | 3 | 2 |
| Yellow, 561 nm | NA | NA | 3 |
| Red, 637 nm | NA | 3 | 3 |
| Total fluorescence detectors available | 9 | 12 | 14 |
| Total parameters per configuration* | 11 | 14 | 16 |
*Includes forward scatter (FSC) and side scatter (SSC). NA = not applicable.
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Table 2. Attune NxT Flow Cytometer configuration options.
| Lasers | Laser configuration (Cat. No.) |
Lasers included, No. of detection channels | Total detection channels* | ||||
|---|---|---|---|---|---|---|---|
| Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm |
Red, 637 nm | |||
| 1 | Blue (A24864) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
| 2 | Blue/green (A28995) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | |
| Blue/yellow (A24861) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | ||
| Blue/red (A24863) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† |
3
|
9 | |
| Blue/violet (A24862) |
4
|
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
| Blue/violet 6 (A29002) |
6
|
3
|
Available as upgrade† | Available as upgrade† | 11 | ||
| 3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
|
12 | |
| Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
| Blue/green/violet (A28999) |
4
|
3
|
4
|
Available as upgrade† | 13 | ||
| Blue/violet/yellow (A24859) |
4
|
3
|
4
|
|
Available as upgrade† | 13 | |
| Blue/red/violet (A24860) |
4
|
4
|
Available as upgrade† | Available as upgrade† |
3
|
13 | |
| Blue/red/violet 6 (A29003) |
6
|
3
|
Available as upgrade† |
3
|
14 | ||
| 4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
|
16 | |
| Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
|
16 | ||
| Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
|
16 | ||
| * Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. |
|||||||
The 2018 Attune NxT Flow Cytometer 6-channel violet laser configuration accommodates the Invitrogen eBioscience Super Bright antibody conjugates along with other commercially available violet-excitable fluorophores.
| Detector | Bandpass (nm) | Fluorophores* |
|---|---|---|
| VL1 | 450/40 | Super Bright 436, Brilliant Violet 421, eFluor 450, Pacific Blue, BD Horizon V450, VioBlue |
| VL2 | 525/50 | eFluor 506, Brilliant Violet 510, Pacific Green, BD Horizon V500, VioGreen |
| VL3 | 610/20 | Super Bright 600, Brilliant Violet 605, Pacific Orange |
| VL4 | 660/20 | Super Bright 645, Brilliant Violet 650 |
| VL5 | 710/50 | Super Bright 702, Brilliant Violet 711 |
| VL6 | 780/60 | Brilliant Violet 786 |
* List is not inclusive of all available fluorophores.
Brochure
Poster
Specification sheet
Table 2. Attune NxT Flow Cytometer configuration options.
| Lasers | Laser configuration (Cat. No.) |
Lasers included, No. of detection channels | Total detection channels* | ||||
|---|---|---|---|---|---|---|---|
| Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm |
Red, 637 nm | |||
| 1 | Blue (A24864) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
| 2 | Blue/green (A28995) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | |
| Blue/yellow (A24861) |
Available as upgrade† |
3
|
4
|
Available as upgrade† | 9 | ||
| Blue/red (A24863) |
Available as upgrade† |
4
|
Available as upgrade† | Available as upgrade† |
3
|
9 | |
| Blue/violet (A24862) |
4
|
4
|
Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
| Blue/violet 6 (A29002) |
6
|
3
|
Available as upgrade† | Available as upgrade† | 11 | ||
| 3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
|
12 | |
| Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
| Blue/green/violet (A28999) |
4
|
3
|
4
|
Available as upgrade† | 13 | ||
| Blue/violet/yellow (A24859) |
4
|
3
|
4
|
|
Available as upgrade† | 13 | |
| Blue/red/violet (A24860) |
4
|
4
|
Available as upgrade† | Available as upgrade† |
3
|
13 | |
| Blue/red/violet 6 (A29003) |
6
|
3
|
Available as upgrade† |
3
|
14 | ||
| 4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
|
16 | |
| Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
|
16 | ||
| Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
|
16 | ||
| * Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. |
|||||||
The 2018 Attune NxT Flow Cytometer 6-channel violet laser configuration accommodates the Invitrogen eBioscience Super Bright antibody conjugates along with other commercially available violet-excitable fluorophores.
| Detector | Bandpass (nm) | Fluorophores* |
|---|---|---|
| VL1 | 450/40 | Super Bright 436, Brilliant Violet 421, eFluor 450, Pacific Blue, BD Horizon V450, VioBlue |
| VL2 | 525/50 | eFluor 506, Brilliant Violet 510, Pacific Green, BD Horizon V500, VioGreen |
| VL3 | 610/20 | Super Bright 600, Brilliant Violet 605, Pacific Orange |
| VL4 | 660/20 | Super Bright 645, Brilliant Violet 650 |
| VL5 | 710/50 | Super Bright 702, Brilliant Violet 711 |
| VL6 | 780/60 | Brilliant Violet 786 |
* List is not inclusive of all available fluorophores.
Brochure
Poster
Specification sheet
Flow Panel Design Tool
Choose antibody conjugates for your panel in 3 steps: 1) pick the antibody species reactivity, 2) select up to 14 targets, and 3) choose the lasers or fluorophores you want to view.
Fluorophore Selection Guide
Use these quick guides to find antibodies and other conjugates of a broad range of fluorescent dyes, fluorescent proteins, Qdot probes, and phycobiliproteins.
Fluorescence SpectraViewer
This online tool allows researchers to assess the spectral compatibility of dyes and probes in the course of designing experiments that utilize fluorescence detection techniques.
Resources
Learning center
Flow Cytometry Learning Center
Access flow cytometry application resources for more success as you plan and execute your experiments.
Support
Flow Cytometry Support Center
Find tips, troubleshooting help, and resources for your flow cytometry workflow.
For Research Use Only. Not for use in diagnostic procedures.