Plasma membrane and mitochondrial membrane changes, caspase activity, and nuclear condensation and fragmentation are all indicators of apoptosis. We've developed reagents and assays for the detection of these events that can be used in combination to help build a more complete picture of apoptosis Find assays for the detection of apoptosis in plasma membrane, mitochondria, cytoplasm and nucleus

Plasma membrane

  Annexin V conjugates Membrane permeability Membrane asymmetry
UV
  • Chromatin Condensation/Dead Cell Apoptosis Kit (V13244)
  • Hoechst 33342 (H3570)
 
405
  • Violet Chromatin Condensation/Dead Cell Apoptosis Kit (A35135)
  • Vybrant DyeCycle Violet (V35003)
  • Violet Ratiometric Asymmetry Probe/ Dead Cell Apoptosis Kit (A35137)
488
  • YO-PRO-1 (Y3603)
  • Membrane Permeability/ Dead Cell Apoptosis Kit (V13243)
 
532    
561    
633  

Mitochondria

  Mitochondrial membrane potential Mitochondrial transition pore
488
  • MitoProbe JC- Assay Kit (M34152)
  • MitoProbe DiOC(2)3 Assay Kit (M34150)
  • Mitochondrial Membrane potential/Annexin V Apoptosis Kit with Mitotracker Red (V35116)
  • MitoProbe Transition Pore Assay (M34153)
532
  • Dihydrorhodamine 123 (D23806)
 
561
  • TMRE (T669)
  • Mitochondrial Membrane potential/Annexin V Apoptosis Kit with Mitotracker Red (V35116)
 
633
  • MitoProbe DiC15 Assay Kit (M34151)
 

Cytoplasm

  Caspase activity
488
  • CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (C10740, C10427)
  • Vybrant FAM Poly Caspases Assay Kit (V35117)
  • Vybrant FAM Caspase-3 and -7 Assay Kit (V35118)
  • Vybrant Caspase-8 Assay Kit (V35119)

Nucleus

  Chromatin condensation DNA fragmentation
UV
  • Chromatin Condensation/Dead Cell Apoptosis Kit (V13244)
  • Hoechst 33342 (H3570)
 
405
  • Vybrant DyeCycle Violet (V35003)
  • Violet Chromatin Condensation/Dead Cell Apoptosis Kit (A35135)
 
488  
  • APO-BrdU TUNEL Assay Kit with Alexa Fluor 488 anti-BrdU (A23210)

Resources

Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Fluorescence SpectraViewer—Assess the spectral compatibility of flurophores.

Support

Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Protocols

Contact us