Since there is no single parameter that defines programmed cell death, a combination of techniques is recommended for the reliable detection of apoptosis. Our multiparametric apoptosis assay kits are designed to selectively differentiate apoptotic cells from living and necrotic cells in a single cell population. The kits are designed to detect various apoptotic parameters in a single flow cytometry assay, helping to dissect the early and middle stages of the apoptotic response.

Multiparametric apoptosis analysis using the Alexa Fluor 488 Annexin V/Dead Cell Apoptosis kit with PI. Jurkat cells (T-cell leukemia, human) were treated with 10 μM camptothecin for four hours (bottom panel) or left untreated as a control (top panel). Cells were then treated with the reagents in the kit and analyzed by flow cytometry. Note that the camptothecin-treated cells (bottom panel) have a higher percentage of apoptotic cells (indicated by “A”) than the basal level of apoptosis seen in the control cells (top panel). L = live cells, D = dead cells.
 Product Parameter 1 Parameter 2 Parameter 3 Excitation (nm) Cat. No.
Single Channel Annexin V/Dead Cell Apoptosis Kit, with Alexa Fluor 488 annexin V and SYTOX Green Membrane Asymmetry Necrosis 488 V13240
Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit, with PI Membrane Asymmetry Necrosis 488 V13241
FITC Annexin V/Dead Cell Apoptosis Kit, with PI Membrane Asymmetry Necrosis 488 V13242
Membrane Permeability/Dead Cell Apoptosis Kit, with YO-PRO-1 and PI Membrane Asymmetry Necrosis 488 V13243
Chromatin Condensation Apoptosis Kit, with Hoechst 33342 and PI Chromatin Condensation Necrosis UV/488 V13244
Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit, with Hoechst 33342, YO-PRO-1, and PI Chromatin Condensation Necrosis UV/488 V23201
PE Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green Membrane Asymmetry Necrosis 488 V35112
APC Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green Membrane Asymmetry Necrosis 488/633 V35113
Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit, with C12 resazurin, APC annexin V, and SYTOX Green Mitochondrial Health Membrane Asymmetry Necrosis 488/633 V35114
Mitchondrial Membrane Potential/Annexin V Apoptosis Kit, with MitoTracker Red and Alexa Fluor 488 annexin V Mitochondrial Health Membrane Asymmetry 488 V35116
Violet Annexin V/Dead Cell Apoptosis Kit, with Pacific Blue annexin V/SYTOX AADvanced Membrane Asymmetry Necrosis 405/488 A35136
Violet Membrane Permeability/Dead Cell 1 Apoptosis Kit, with PO-PRO-1 and 7-aminoactinomycin D Membrane Asymmetry Necrosis 405/488 V35123
Violet Chromatin Condensation/Dead Cell Apoptosis Kit, with Vybrant DyeCycle Violet and SYTOX AADvanced Chromatin Condensation Necrosis 405/488 A35135

Resources

Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.

Fluorescence SpectraViewer—Assess the spectral compatibility of flurophores.

Support

Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.

Flow Cytometry Protocols

Contact us