Invitrogen eBioscience Super Bright 645 dye is tandem polymer dye, consisting of violet (405 nm) laser–excitable Super Bright 436 and an acceptor dye that emits fluorescence signal at 645 nm. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry because they emit fluorescence with high efficacy. 

An alternative to Brilliant Violet 650 conjugates, antibody conjugates of Super Bright 600 provide increased resolution of positive and negative populations, but with less background and less interfering dye–dye interaction than Brilliant Violet 650 conjugates.

Executing a well-designed experiment requires multiple reagents.  Super Bright antibody conjugates are fully compatible with Invitrogen UltraComp eBeads microspheres for compensation, viability stains, fluorescent dyes and other reagents commonly used in flow cytometry. Robust, highly sensitive, and stable, Super Bright conjugates easily fit into any flow cytometry panel.

We offer Super Bright 645 dye conjugated to primary antibodies for use in flow cytometry. 

Super Bright 645 dye dashboard


Initial brightness

 
 
 
 
 

 

The violet laser–excitable Super Bright 645 dye is optimized for use in flow cytometry applications.

 
   

Photostability in buffer

 
 
 
 
 

  405 660/20 414 645  
 
Laser line

Common filter set

Excitation max

Emission max
 

Super Bright 645 dye products

We offer Super Bright 645 dye conjugated to primary antibodies for use in flow cytometry.

Learn more here:

Primary antibodies for the violet laser

Invitrogen eBioscience Super Bright polymer dyes are violet laser–excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals.

The Super Bright polymer dyes join the trusted Invitrogen Alexa Fluor dye and Invitrogen eFluor dye portfolios in providing fluorochromes compatible with the 405 nm spectral line of the violet laser.

Resources

Primary antibodies for the violet laser

Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

Learn more about primary antibodies for the violet laser

Fluorescence SpectraViewer

Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

Go to the Fluorescence SpectraViewer