We offer a number of Invitrogen Molecular Probes labeling kits for the direct attachment of intensely fluorescent Invitrogen Alexa Fluor dyes, Invitrogen Qdot labels, R-phycoerythrin (R-PE), or even biotin to less than 10 μg up to 1 mg of IgG antibody.

Directly labeled antibodies allow you to use more than one same-species antibody in a single staining experiment. You can use traditional labeling chemistries optimized for your application or site-specific labeling using click-chemistry technology.

Site-specific antibody and protein labeling

How much antibody would you like to label?

<1–20 µg

Zenon Labeling Technology provides a rapid, versatile, and reliable method for producing non-covalent, fluorescently labeled antibodies, targeting just the Fc portion of the primary antibody. In a ten-minute reaction protocol, Zenon Labeling Technology works with even small amounts of unpurified starting material.

<100 µg

SiteClick Labeling Technology  specifically attaches the label to the heavy chains of an IgG antibody ensuring that the antigen binding domains remain available for binding to your antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies regardless of isotype and host species. Depending upon the label, the resulting SiteClick labeled antibody can be used in flow cytometry, fluorescence imaging, or western blot detection.

Conventional antibody and protein labeling

How much antibody would you like to label?

10–20 µg

APEX Antibody Labeling Kits provide an efficient, convenient and reliable method for covalently attaching fluorescent labels to very small amounts of IgG antibody in the presence of any contaminants including stabilizing proteins and amine-containing buffers.

20–100 µg

Microscale Protein Labeling Kits conveniently label small amounts of purified protein or antibodies (MW between 12 and 150 kDa) with a bright, photostable Invitrogen Alexa Fluor dye or the hapten, biotin-XX.

100 µg

Directly label monoclonal and polyclonal antibodies with Invitrogen Molecular Probes Antibody Labeling Kits and eliminate background staining commonly observed with secondary antibodies and use more than one same-species antibody in a single experiment.

1 mg

Our Protein Labeling Kits provide a nearly effortless way to stably label larger amounts of IgG antibody with a fluorescent dye or hapten in 2 hours.

0.5–3 mg

The SAIVI Rapid Antibody Labeling Kits are uniquely designed to provide the rigorous requirements to control the degree of labeling (DOL) using our near-infrared (NIR) Invitrogen Alexa Fluor dyes.

Resources

For Research Use Only. Not for use in diagnostic procedures.