How Zenon labeling technology works
Zenon labeling technology provides a versatile, easy-to-use system for labeling human, mouse IgG1, IgG2a, and IgG2b antibodies and rabbit IgG antibodies. Zenon® fragments are specifically designed to target and bind to the Fc portion of the primary antibody only, giving a rapid, noncovalent method of quickly labeling small quantities of primary antibody (Figure 1). Zenon labeling technology is simple and efficient; the entire labeling procedure takes only 10 minutes.
Once the Zenon–antibody complex has formed, it can be stored until needed; just prior to use, nonspecific IgG is added to block any unbound Zenon fragments, making column purification unnecessary. The Zenon complex can then be applied directly to the sample.
Antibodies labeled using Zenon labeling technology display fluorescence intensity or enzymatic activity similar to that observed for directly labeled antibody conjugates (Figure 2).
Ultimate flexibility with Zenon Antibody Labeling Kits
Zenon labeling technology offers unique flexibility for when you want to change colors or test whether a particular fluorophore is suitable for your application or antibody. The extensive selection of labels and the versatility of this labeling technology make it easy to experiment with different color combinations for multicolor applications in both flow cytometry (Figure 3) and fluorescence imaging (Figures 4 and 5).