Triple flask inside incubator

More surface, same footprint for optimal scale-up and automation

Continue your scale-up with consistency. Our triple layer flask features the same cell culture certified surface used in our range of T25 to T225 flasks, and provides a total of 500 cm2 surface area for cell attachment , but with the footprint of a T175 flask. It's a large flask with a small footprint. Your lab can enjoy the benefits of minimized variability of cell growth during scale up, saved incubator space, improved lab productivity and compatibility for automated cell culture.

Sign up to receive our informative publications featuring blogs, webinars and more, as soon as they're available.

Subscribe to cell culture updates ›


Quick links

What makes our Nunc TripleFlasks the right solution for scale up and cell culture automation?

Multiple layers in one flask

Filter or Vent/Close cap

Triple layer flask
Triple layer flask footprint
Triple flask caps

Engineered for lab productivity

  • Provides 500 cm2 surface area for cell attachment with the footprint
    of a T175 flask
  • Compact, triple-layer design delivers expanded culture surface to help
    save CO2 incubator space and increase lab productivity
  • Utilizes proprietary Nunclon Delta™ surface treatment for maximum
    adhesion of a broad range of cell types
  • Engineered to support automated cell culture and large cell mass
    applications such as drug screening
  • Offered barcoded with large Code 128

Filter caps

  • Effective barrier against micro-organisms
  • Continuous venting with consistent gas exchange

Vent/close caps

  • “Y” mark allows visual verification of vent position - open or closed

T500 triple layer flasks for scale up of adherent cultures

Our multi-layer flasks are cell culture certified with the Nunclon Delta surface treatment to support optimal attachment of a broad range of cell types. The 500 cm2 surface area flask is on our continuum of cell culture certified vessels with growth surfaces ranging from 0.13 cm to 32,864 cm2, allowing for easy scale up when expanding cultures. 

View full-size scalability chart ›

  Neck Surface Material Cap Cap Feature
TripleFlask Straight, ideal for automated cell culture Nunclon Delta Cell culture-treated polystyrene Filter sufficient gas exchange
TripleFlask Straight, ideal for automated cell culture Nunclon Delta Cell culture-treated polystyrene Vent/Close constant airflow

T500 triple layer flasks barcoded for automated cell culture

For consistency in cell culture scale up and automation, barcoded triple layer flasks are certified Nunclon Delta treated, offering 500 cm2 surface area on our continuum of cell culture certified vessels with growth surfaces spanning 0.13 cm2 to 32,864.

  Neck Surface Material Cap Cap Feature
TripleFlask Straight, ideal for automated cell culture Nunclon Delta Cell culture-treated polystyrene, barcoded Filter constant airflow

T500 triple layer flasks for suspension cell culture

Made with high quality, optically clear virgin polystyrene, the Thermo Scientific Nunc Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells.

  Neck Surface Material Cap Cap Feature
TripleFlask Straight, ideal for automated cell culture Not cell culture treated Untreated polystyrene for suspension growth of non adherent cells Filter constant airflow

How to distribute your cell suspension among multiple growth surfaces

Triple flask culturing technique diagram

Culturing technique is the key. Start by pouring a homogenous cell suspension carefully into the triple-layer flask. After letting the volume equilibrate among the three layers in the upright position, hold the flask on its side, at an approximately 75° angle to the workbench, before gently and quickly laying it down on its growth surface. The angled back-tilting enables equal distribution of the cell suspension among the three layers.

Find step-by-step instructions in our featured SmartNote.

View Smart Note ›

 

 

About your cell culture certified T500 flask surface

showcase.par.27579

The Thermo Scientific Nunclon Delta cell culture surface
The Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. The Nunclon Delta certificate ensures high quality standards and lot-to-lot consistency. Look for it on a variety of Thermo Scientific cell culture plastics. 

To help ensure flexible, reproducible and reliable results across every stage of cell culture, we offer a range of cell culture flasks spanning a variety of formats, sizes and surfaces. 

For additional guidance on surface usage by cell type, view our
Surface Selection Guide.

Related flask and cell culture resources

Surface selection: Achieving optimal cell growth and harvesting

Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.

  • Surface modifications to improve cell adhesion
  • Characterization of Nunc cell culture surfaces by adhesion properties
  • Alternative methods for advanced cell harvesting

Watch now

Watch more cell culture webinars

Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.

Visit the café

Resources

Application notes

Brochures