Insect Cell Culture

Insect cell culture is a common choice for heterologous protein expression.  For large scale production or basic research, insect cells are able to express large quantities of protein with complex post-translational modifications.

Gibco™ insect media has been formulated for maximum growth and protein yields.  This media, in combination with Gibco pre-adapted cell lines, will provide a convenient system to save you time and effort.

Which insect media is right for you?

Basic Insect
Cell Research
Serum-Free Growth & Protein Expression Highest Consistency & Protein Expression
Grace's Insect Media Sf-900™ II
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Sf-900™ III
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Serum Free 
Protein Free 
Source originAnimalSerum FreeAnimal Origin-Free
Lot-to-lot consistencyLow (FBS variability)Medium (medium hydrolysate concentration)High (low hydrolysate concentration)
Optimized to prevent foaming 
Compatible insect cellsSf9 & Sf21Sf9 & Sf21Sf9 & Sf21
Supported Cell Density (Sf9 cells/mL)3 x 106 cells/mL 10 x 106 cells/mL 10–14 x 106 cells/mL
Packaging100 mL, 500 mL, 10 x 1L (powder)500 mL, 1000 mL, 5 L (bags), 10 L (bags), 20 L (bags)500 mL, 1000 mL, 10 L (bags), 20 L (bags)
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Drosophila S2 Cell Culture

Drosophila S2 cells are used for heterologous protein expression in the Drosophila Expression System (DES). The S2 cell line was derived from a primary culture of late stage Drosophila melanogaster embryos (Schneider, 1972). This adaptable cell line grows without CO2, at room temperature, as a semi-adherent monolayer or suspension in Scheider's Drosophila Medium.

High Five™ Cell Culture

High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate, derived from the parental Trichoplusia ni cell line. They are optimized for the growth and maintenance of cells used with the Baculovirus expression vector system (BEVS) for adherent or suspension culture. Adapted to serum-free suspension culture in Gibco Express Five™ serum free medium.


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