Transfection is the process by which nucleic acids are introduced into mammalian cells. Protocols and techniques vary widely and include lipid transfection and chemical and physical methods such as electroporation. Expertise in cellular and molecular biology is behind the Invitrogen product portfolio of DNA reagents and consumables, which are the most cited and trusted transfection and selection systems available. Use this overview of our most effective transfection products to choose the solution that’s right for you

See the Transfection Reagent Selection Guide

Featured application note: Stem Cell Transfection Guide

Stem cells continue to show immense promise for the future of regenerative medicine and personalized therapeutic treatments. Improved delivery of DNA, RNA, or protein using validated solutions can help expedite the progress of research leading to new therapies. We have created the following guide as a starting point for the transfection of various types of stem cells. Our goal is to provide a holistic view of the options, trade-offs, and delivery recommendations for a range of stem cell models.  Download this Guide to  learn more. 

Download the new Stem Cell Transfection Guide

Transfection Products

By sample type

By cell type

Our protocols for Lipofectamine Reagent have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially with a new cell line.

If you are working with cancer cell lines, we’ve developed and validated cancer cell line-specific protocols covering the most researched cell lines in breast cancer, lung cancer, and liver cancer.

If neither of these options is working for your specific cell type, consult our additional cell type–specific protocols for further optimization.

Transfection Reagents

Designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures. Lister below are Invitrogen product lines:

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Transfection Methods

Resources

Education & Tools

Related Applications

Transfection Protocols

Related Products