Get started with cloning

We offer powerful and versatile Invitrogen™ cloning and expression vectors, GeneArt™ Gene Synthesis and assembly tools, and molecular biology essentials for that critical first step in your experiment. Unsure where to start? Find out more

An introduction to cloning

Most popular cloning products

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Cartoon of synthetic DNA
Cartoon of engineered chromosome

Featured cloning categories

5-minute, room-temperature cloning with no gel purification or post-PCR modifications & up to 95% recombinants with TOPO cloning.

Combining the convenience of the Invitrogen online ordering system, with cutting-edge GeneArt service offerings.

High-efficiency cloning of blunt-ends using Zero Blunt® cloning kits.

Based on Type IIs restriction enzymes for non-recombination-based cloning of multiple DNA inserts.

Easily clone fragments using TOPO XL™ & GeneArt Seamless Cloning technology.

Gateway recombination cloning technology allows gene shuttling, enabling you to access virtually any expression system.

TA cloning technology relies on the complimentary of thymine & adenine. Enhanced with ExpressLink™ T4 DNA Ligase.

Choose competent cells based on application, strain, packaging format & transformation efficiency.

Browse our new one-buffer Anza restriction enzyme cloning system as well as our Invitrogen conventional restriction and modifying enzymes.

PCR enzymes for any application: high fidelity, high specificity, long fragment, GC-rich, genotyping, or routine PCR.

A variety of scales, formats, modifications, & purifications to meet your cloning needs.

Industry-leading sequence analysis & data management software.

Clone up to 10 DNA fragments simultaneously; assemble existing fragments without restriction digest or PCR amplification.

FastDigest™ restriction enzymes, CloneJET™ kits, InsTAclone™ kits, Ligase Independent Cloning, Alkaline Phosphatases and more.

Cloning resources

NEW Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications

We offer everything from custom vector design to high-throughput protein expression.

Tools

Molecular Cloning Webinars