Learn the basics of traditional cloning and common troubleshooting strategies for ensuring successful cloning experiments.

Traditional cloning basics

Conditions for using restriction endonucleases for traditional cloning to ensure successful restriction digestion, ligation and colony screening are explained here.

Common cloning applications and strategies

Learn about various cloning strategies, including PCR cloning, subcloning, genomic and cDNA library construction, and shotgun sequencing.

Cloning troubleshooting guide

Learn common troubleshooting strategies for basic cloning methods including proper controls to evaluate ligation and transformation problems.